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Eagle Eye Optical Filter Optimized for Imaging Silver-Stained,,,Gels

1 shows a gel as imaged by the system using either the ethidium bromide (panel A) or silver stain (panel B) optical filter with transillumination. Figure 2 shows an image of the gel obtained using the same filters but with overhead illumination. Using either transillumination or overhead illumination, the images captured using the silver-stain filter demonstrate an increased contrast over images obtained using the standard Eagle Eye filter; consequently, certain faint bands were more easily visualized. For example, proteins migrating at about 40 kDa are clearly visible in lanes 4 and 5 (earlier-eluting column fractions) when the silver-stain filter was used but do not become visible until lanes 7 and 8 (later-eluting fractions) when the ethidium bromide filter was used. The bands recorded using the silver-stain filter closely reflect what can be seen on the gel by eye. Although contrast and sensitivity were enhanced for most protein bands on the gel, those with a brown-orange tone showed the greatest improvement.

Figure 2

Conclusions

The silver-stain optical filter can improve image contrast when imaging silver-stained gels with the Eagle Eye II Still Video System. Using this filter can lead to improved visualization of faint bands, which increases the sensitivity of the imaging system or may reduce the amount of protein required for analysis.

Acknowledgements

The authors thank Frances Bai and Brad Scott for providing silver-stained SDS PAGE gels for imaging.

REFERENCES
  1. Morrissey, J.H. (1981) Analytical Biochemis
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