Navigation Links
Eagle Eye Optical Filter Optimized for Imaging Silver-Stained,,,Gels


New filter designed for optimal image clarity

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

Michelle Cayouette Jane Moores
Stratagene

A new optical filter has been developed for the Eagle Eye II Still Video System that increases contrast and sensitivity when imaging silver-stained protein gels.

The Eagle Eye II Still Video System is currently sold with an optical filter optimized for imaging nucleic acid gels stained with ethidium bromide. Because the system is also frequently used to image silver-stained SDS-PAGE gels,1 we designed an optical filter to improve contrast and sensitivity when imaging these gels. Thus, Stratagenes optical filter line now comprises ethidium bromide, SYBR Green,2 Coomassie Blue,3 and silver-stain filterseach customized for different applications. All filters are coated to ensure high image quality.

Comparison of Optical Filters

SDS-PAGE protein gels were silver stained using standard procedures and were then used to compare the performances of the silver-stain and ethidium bromide filters. A stained gel was first positioned on a white-light transilluminator and imaged with the Eagle Eye II Still Video System, using each filter in turn. The same gel was then positioned on a piece of shiny, white plastic and imaged using the overhead white lights in the Eagle Eye cabinet. In each case, the f-stop was adjusted until the displayed image represented the gel as well as possible.

Figure 1

Figure 1 shows a gel as imaged by the system using either the ethidium bromide (panel A) or silver stain (panel B) optical filter with transillumination. Figure 2 shows an image of the gel obtained using the same filters but with overhead illumination. Using either transillumination or overhead illumination, the images captured using the silver-stain filter demonstrate an increased contrast over images obtained using the standard Eagle Eye filter; consequently, certain faint bands were more easily visualized. For example, proteins migrating at about 40 kDa are clearly visible in lanes 4 and 5 (earlier-eluting column fractions) when the silver-stain filter was used but do not become visible until lanes 7 and 8 (later-eluting fractions) when the ethidium bromide filter was used. The bands recorded using the silver-stain filter closely reflect what can be seen on the gel by eye. Although contrast and sensitivity were enhanced for most protein bands on the gel, those with a brown-orange tone showed the greatest improvement.

Figure 2

Conclusions

The silver-stain optical filter can improve image contrast when imaging silver-stained gels with the Eagle Eye II Still Video System. Using this filter can lead to improved visualization of faint bands, which increases the sensitivity of the imaging system or may reduce the amount of protein required for analysis.

Acknowledgements

The authors thank Frances Bai and Brad Scott for providing silver-stained SDS PAGE gels for imaging.

REFERENCES
  1. Morrissey, J.H. (1981) Analytical Biochemis try 117: 307-310.
  2. Cayouette, M. and Moores, J. (1997) Strategies 9: 88-89.
  3. Cayouette, M. and Moores, J. (1997) Strategies 10: 44-45.

'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
2. Near Infrared Technology and Optical Agents For Molecular Imaging
3. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
4. Optimized Dengue RT-PCR
5. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
6. Imaging Chemiluminescent Western Blots
7. Imaging and Analysis of DIGE Product Information Sheet, Rev B
8. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
9. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
10. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
11. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/12/2017)... JOHNSTON, Iowa (PRWEB) , ... October 12, 2017 ... ... company based in Vilnius, Lithuania, announced today that they have entered into a ... collaboration is to provide CRISPR researchers with additional tools for gene editing across ...
(Date:10/11/2017)... ... October 11, 2017 , ... At its ... announced Dr. Suneel I. Sheikh, the co-founder, CEO and chief research scientist of ... been selected for membership in ARCS Alumni Hall of Fame . ASTER ...
(Date:10/11/2017)... -- VMS BioMarketing, a leading provider of patient support solutions, has ... (CNE) network, which will launch this week. The VMS CNEs ... professionals to enhance the patient care experience by delivering peer-to-peer ... care professionals to help women who have been diagnosed and ... ...
(Date:10/10/2017)... ... , ... San Diego-based team building and cooking events company, Lajollacooks4u, has unveiled ... bold new look is part of a transformation to increase awareness, appeal to new ... , It will also expand its service offering from its signature gourmet cooking classes ...
Breaking Biology Technology:
(Date:4/3/2017)... 2017  Data captured by IsoCode, IsoPlexis ... a statistically significant association between the potency ... and objective response of cancer patients post-treatment. ... whether cancer patients will respond to CAR-T ... as to improve both pre-infusion potency testing and ...
(Date:3/29/2017)... , March 29, 2017  higi, the health IT ... North America , today announced a ... the acquisition of EveryMove. The new investment and acquisition ... of tools to transform population health activities through the ... data. higi collects and secures data today ...
(Date:3/24/2017)... Research and Markets has announced the addition ... Trends - Industry Forecast to 2025" report to their offering. ... The Global Biometric ... of around 15.1% over the next decade to reach approximately $1,580 ... market estimates and forecasts for all the given segments on global ...
Breaking Biology News(10 mins):