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Eagle Eye Optical Filter Optimized for Imaging Silver-Stained,,,Gels


New filter designed for optimal image clarity

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

Michelle Cayouette Jane Moores
Stratagene

A new optical filter has been developed for the Eagle Eye II Still Video System that increases contrast and sensitivity when imaging silver-stained protein gels.

The Eagle Eye II Still Video System is currently sold with an optical filter optimized for imaging nucleic acid gels stained with ethidium bromide. Because the system is also frequently used to image silver-stained SDS-PAGE gels,1 we designed an optical filter to improve contrast and sensitivity when imaging these gels. Thus, Stratagenes optical filter line now comprises ethidium bromide, SYBR Green,2 Coomassie Blue,3 and silver-stain filterseach customized for different applications. All filters are coated to ensure high image quality.

Comparison of Optical Filters

SDS-PAGE protein gels were silver stained using standard procedures and were then used to compare the performances of the silver-stain and ethidium bromide filters. A stained gel was first positioned on a white-light transilluminator and imaged with the Eagle Eye II Still Video System, using each filter in turn. The same gel was then positioned on a piece of shiny, white plastic and imaged using the overhead white lights in the Eagle Eye cabinet. In each case, the f-stop was adjusted until the displayed image represented the gel as well as possible.

Figure 1

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