Navigation Links
END SEQUENCING

Agencourt offers high quality end sequencing for BACs, fosmids and cosmids with a throughput capacity of 25,000 end reads per day. The key features of our end sequencing service are:
  • Industry leading sequence quality
  • Increased linkage rates
  • Rapid sample turnaround
  • Fully-automated, GLP-compliant facility

END SEQUENCING SERVICE

Our end sequencing service includes:

  • Template purification
  • Sequence reaction set up and thermocycling
  • Dye-terminator removal
  • Sequence delineation on an
    ABI PRISM 3700/3730xl
  • Base calling and data compilation
  • Data delivery via a secure FTP site

COMPREHENSIVE AND PROVEN PIPELINE

Agencourts comprehensive pipeline allows for effortless transition from end sequencing to complete insert sequence delineation. We have extensive experience with both end sequencing and full length insert sequencing of low copy vectors. The Whitehead Institute acknowledged the throughput and quality of Agencourts end sequencing services by collaborating with us on a project to end sequence 200,000 Ciona savignya and mouse fosmid clones, a project we completed in 2001.

INCREASED LINKAGE RATES

Linkage rates are a key parameter when assessing end sequencing success. Linkage of forward and reverse sequences is dependent on both sequencing pass rates and sample tracking. Poor sample tracking and plate management can result in low linkage rates despite high pass rates (Figure 2A). Agencourts Galaxy LIMS and distinct plate set up allow sample tracking rates of greater than 99%, while incorporation of SPRI chemistry within our workflow consistently produces average pass rates in excess of 85% (Figure 2B). Our accurate sample tracking and high sequencing pass rates lead to increased linkage rates which are typically greater than 75%. Increased linkage rates result in fewer reads per project for our clients, allowing increased sample throughput and reduced project cost.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. FULL LENGTH INSERT SEQUENCING
2. WHOLE GENOME SEQUENCING
3. SAGE & LONGSAGE SEQUENCING
4. SINGLE PASS SEQUENCING
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... WA (PRWEB) , ... June 23, 2016 , ... ... announces the release of its second eBook, “Clinical Trials Patient Recruitment and Retention ... recruitment and retention in this eBook by providing practical tips, tools, and strategies ...
(Date:6/23/2016)... Mass. , June 23, 2016   ... development of novel compounds designed to target cancer ... napabucasin, has been granted Orphan Drug Designation from ... the treatment of gastric cancer, including gastroesophageal junction ... stemness inhibitor designed to inhibit cancer stemness pathways ...
(Date:6/23/2016)... -- A person commits a crime, and the detective uses ... criminal down. An outbreak of foodborne illness makes ... uses DNA evidence to track down the bacteria that caused ... not. The FDA has increasingly used a complex, cutting-edge technology ... Put as simply as possible, whole genome sequencing is a ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... Plate® YM (Yeast and Mold) microbial test has received AOAC Research Institute approval ... of microbial tests introduced last year,” stated Bob Salter, Vice President of Regulatory ...
Breaking Biology Technology:
(Date:6/9/2016)... Finland , June 9, 2016 ... National Police deploy Teleste,s video security solution to ensure the ... France during the major tournament ... data communications systems and services, announced today that its video ... Prefecture to back up public safety across the ...
(Date:6/2/2016)... -- The Department of Transport Management (DOTM) of ... US Dollar project, for the , Supply and ... and IT Infrastructure , to Decatur ... of Identity Management Solutions. Numerous renowned international vendors participated in ... was selected for the most compliant and innovative solution. ...
(Date:6/1/2016)... , June 1, 2016 Favorable ... Election Administration and Criminal Identification to Boost Global Biometrics ... recently released TechSci Research report, " Global Biometrics Market ... Competition Forecast and Opportunities, 2011 - 2021", the global ... by 2021, on account of growing security concerns across ...
Breaking Biology News(10 mins):