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END SEQUENCING

Agencourt offers high quality end sequencing for BACs, fosmids and cosmids with a throughput capacity of 25,000 end reads per day. The key features of our end sequencing service are:
  • Industry leading sequence quality
  • Increased linkage rates
  • Rapid sample turnaround
  • Fully-automated, GLP-compliant facility

END SEQUENCING SERVICE

Our end sequencing service includes:

  • Template purification
  • Sequence reaction set up and thermocycling
  • Dye-terminator removal
  • Sequence delineation on an
    ABI PRISM 3700/3730xl
  • Base calling and data compilation
  • Data delivery via a secure FTP site

COMPREHENSIVE AND PROVEN PIPELINE

Agencourts comprehensive pipeline allows for effortless transition from end sequencing to complete insert sequence delineation. We have extensive experience with both end sequencing and full length insert sequencing of low copy vectors. The Whitehead Institute acknowledged the throughput and quality of Agencourts end sequencing services by collaborating with us on a project to end sequence 200,000 Ciona savignya and mouse fosmid clones, a project we completed in 2001.

INCREASED LINKAGE RATES

Linkage rates are a key parameter when assessing end sequencing success. Linkage of forward and reverse sequences is dependent on both sequencing pass rates and sample tracking. Poor sample tracking and plate management can result in low linkage rates despite high pass rates (Figure 2A). Agencourts Galaxy LIMS and distinct plate set up allow sample tracking rates of greater than 99%, while incorporation of SPRI chemistry within our workflow consistently produces average pass rates in excess of 85% (Figure 2B). Our accurate sample tracking and high sequencing pass rates lead to increased linkage rates which are typically greater than 75%. Increased linkage rates result in fewer reads per project for our clients, allowing increased sample throughput and reduced project cost.


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Related biology technology :

1. FULL LENGTH INSERT SEQUENCING
2. WHOLE GENOME SEQUENCING
3. SAGE & LONGSAGE SEQUENCING
4. SINGLE PASS SEQUENCING
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