Navigation Links
EL 4

ing so, set the cell concentration to 3.8 x 106 cells/ml.
  • Add and mix plasmid DNA (25 g/ml final concentration, in bidistilled H2O).
  • Transfer 800 l cell suspension into electroporation cuvettes (4 mm gap width). The cell suspension must be free of air bubbles.
  • Electroporation:

    Mode Eukaryotes Voltage (V) 480 V Time constant (T) 50 s No. of pulses (n) 1
  • After the pulse, allow the cell suspension to stand in the cuvette for 5-10 minutes at room temperature.
  • Carefully transfer the cell suspension from the cuvette to 5 ml RPMI 1640 / 0.5% FCS, and centrifuge it for 5-10 minutes, 200 x g, at room temperature. Remove supernatant.
  • Resuspend the cells in 3 ml RPMI 1640 / 10% FCS, and cultivate them in a 55 mm culture dish.
    • Detection methods for transfection:
    The expression of the plasmid pEGFP-N1 can be detected clearly after 24-48 hours with the aid of FACS analysis or under a fluorescence microscope. Result: Survival rate: 81% Transfection rate: 40% based on the number of surviving cells
    32% based on the initial number of cells used for the experiment Results were measured 48 hours after transfection.


    '"/>

    Source:


    Page: All 1 2

    Related biology technology :

    1. Escherichia coli C600
    2. Escherichia coli DH10B
    3. Escherichia coli DH5
    4. Escherichia coli K12
    5. Escherichia coli C600
    6. Escherichia coli K12
    7. Escherichia coli DH10B

    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    		TAG:

    (Date:11/6/2009)... AURORA, Ontario, Nov. 6 ...ets: HXBPF) today announced that John Docherty, pr...at Merriman Curhan Ford,s 6th annual Investor Summ...Hotel in New York City. Mr. Docherty will provide...lopment programs L-DOS47 and Topical Interferon Al...
    (Date:11/6/2009)... CALGARY, Nov. 6 /PRNewswire-FirstCall/ - Oncolyti...s" or the "Company") today announced updated resul...) in patients with sarcomas metastatic to the lung...ctive Tissue Oncology Society Meeting held in Miam...ster presentation, entitled "A Phase II Study of I...
    (Date:11/5/2009)... WELLINGTON, Colo., Nov. 5 The end...owns in the western U.S. This rare carnivore feed...ceptible to plague and often entire population die...e Service (FWS) has implemented a ferret breeding ...rrets into their formerly native habitat. Unfortu...
    (Date:11/5/2009)... OMAHA, Neb., Nov. 5 Tra...nnounced financial results for the quarter ended S... are presented in the tables that follow. ,, T...t loss of $366,000 or $0.01 per share for the thir...9,000 or $0.01 per share for the third quarter of ...
    Breaking Biology Technology:Helix BioPharma to Present at Merriman Curhan Ford's Investor Summit 2009 on November 10th 2Helix BioPharma to Present at Merriman Curhan Ford's Investor Summit 2009 on November 10th 3Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 2Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 3Colorado Companies Team Up With U.S. Fish & Wildlife Service to Protect Black-Footed Ferret 2Transgenomic, Inc. Reports Third Quarter 2009 Results 2Transgenomic, Inc. Reports Third Quarter 2009 Results 3Transgenomic, Inc. Reports Third Quarter 2009 Results 4Transgenomic, Inc. Reports Third Quarter 2009 Results 5Transgenomic, Inc. Reports Third Quarter 2009 Results 6Transgenomic, Inc. Reports Third Quarter 2009 Results 7
    ...ducation has historically been associated with tel...ited high school , , ,Source: KSCADE MADISON Fo...th television commercials promising degrees in fie... all earned over the mail, from home. This is no l... forefront of utilizing advances in high-speed net...
    ...day, Dr. Mark Polczynski is the technology consult... Corporation. But on evenings and weekends, he,s ...ss, M and E Irish Flutes, is not a huge money ma...But the time he has spent developing and running a...ynski a great deal about the importance of keeping...
    ...ier this summer, we looked at venture capital fund...which reached about $5 billion in investments in s... lower than the $5.4 billion invested during the f...seCooper,s MoneyTree analysis. , ,The results for...ve shocker as venture capital investments achieved...
    Other Biology Technology:Charter Business powers distance learning 2Charter Business powers distance learning 3Charter Business powers distance learning 4Irish flute passion drives e-commerce spirit for Eaton techie 2Irish flute passion drives e-commerce spirit for Eaton techie 3Midwest life science companies capture 3% of VC investments in Q.2 2
    (Date:11/4/2009)...TY, Calif., Oct. 20 DigitalPersona...ntication solutions, today announced that WAND Cor...) software for quick-service restaurants, has inte...n POS and Classic POS solutions. WAND,s quick-serv...o boost cashier accountability and deter unauthori...
    (Date:11/4/2009)...Oct. 27 Global Rainmakers, Inc., a...ed R&D laboratory, today announced that the co...d to actively commercialize its suite of technolog...curity offerings. ,, During the past four years...s to develop and deploy a series of core--but expa...
    (Date:11/3/2009)...N, FL (November 4, 2009) For more than 25 years, ...nch ( Strombus gigas ) have been unsuccessfuluntil...eding techniques to produce beaded (nucleated) and...ave been developed by scientists from Florida Atla...itute (HBOI). With less than two years of research...
    Breaking Biology News(10 mins):WAND Point-of-Sale Solutions Integrate U.are.U(R) Fingerprint Biometrics From DigitalPersona for Quick-Service Restaurants 2WAND Point-of-Sale Solutions Integrate U.are.U(R) Fingerprint Biometrics From DigitalPersona for Quick-Service Restaurants 3Global Rainmakers Brings Cutting-Edge Biometric Security Products to Reality 2Global Rainmakers Brings Cutting-Edge Biometric Security Products to Reality 3Scientists are first to 'unlock' the mystery of creating cultured pearls from the queen conch 2Scientists are first to 'unlock' the mystery of creating cultured pearls from the queen conch 3William Stanfield MD Is First Cleveland area Orthopedic Surgeon to Perform OtisKnee Custom Fit Knee Replacement 13481 1William Stanfield MD Is First Cleveland area Orthopedic Surgeon to Perform OtisKnee Custom Fit Knee Replacement 13481 2William Stanfield MD Is First Cleveland area Orthopedic Surgeon to Perform OtisKnee Custom Fit Knee Replacement 13481 3William Stanfield MD Is First Cleveland area Orthopedic Surgeon to Perform OtisKnee Custom Fit Knee Replacement 13481 4Community Hospital System Integrates Patient Data with Orion Health 54139 1Community Hospital System Integrates Patient Data with Orion Health 54139 2Community Hospital System Integrates Patient Data with Orion Health 54139 3ZyDoc Medical Transcription Completes Acquisition of Administrative Advantage 54135 1ZyDoc Medical Transcription Completes Acquisition of Administrative Advantage 54135 2ZyDoc Medical Transcription Completes Acquisition of Administrative Advantage 54135 3ZyDoc Medical Transcription Completes Acquisition of Administrative Advantage 54135 4ZyDoc Medical Transcription Completes Acquisition of Administrative Advantage 54135 5
    ...d in the open-access journal PLoS Biology shows st...n orangutan populations living in the fragmented f...abah, Malaysia. Benoit Goossens, Lounès Chikhi, ...t the collapse occurred within the past hundred ye...ciding with massive deforestation, which began in ...
    ...nique to ensure complete tumor removal at breast c...adiology. , Researchers used light waves in a newl...-the terahertz region--to examine excised breast t...were clear of cancer, with good results. This tech... multiple surgeries and tissue samples to get clea...
    ...s, packed with wildly different species, evolution...tations, some adapt particularly well to their env...ts, mountain forests ?and, as a new study illustra...lished online today in Nature Genetics, Carlo Male...d his colleagues report that precancerous tumors c...
    Other Biology News:Genetic study shows humans have pushed orangutans to the brink of extinction 2Terahertz imaging may reduce breast cancer surgeries 2Researchers create pigs that produce heart-healthy omega-3 fatty acids 2
    Rabbit Anti-Rat Sphingolipid Receptor Edg8 / S1P5 Polyclonal Antibody, Unconjugated from IMGENEX
    Mitochondria / Cytosol Fractionation Kit from MBL International
    ...collection and automation that is controlled from ...th Microsoft Excel , Pre-configured library of in...ty meters, force gauges, spectrophotometers, etc ...d configured by the user , Collect data from mul...
    BAC Sequencing from Bio S&T
    Biology Products: