Navigation Links
EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY


The spectra in Figure 3 have been offset by 0.1A in order to show the enhanced details in the spectrum of serum albumin measured in the 1nm bandwidth instrument. With enhanced resolution, the contributions from phenylalanine, tyrosine, and tryptophan become more obvious. These residues absorb between 260 and 280nm. However, as was the case for the sample of pure DNA, there was little difference in the measured absorbance (~0.6) and wavelength maximum (~278nm) of the protein sample in the two instruments.

A much different situation applies when DNA purity is assessed. In this situation, the instrument must discriminate between DNA and likely contaminants. These contaminants include protein, chloroform, phenol. Contamination by protein is usually indicated by a decrease in the A260/A280 ratio. Contamination by an extraction solvent such as phenol or chloroform is indicated by increased absorbance at 320nm. If the criterion for acceptable resolution of instrument bandwidth ten-fold less than peak wavelength difference (280-260nm = 20nm) is applied to samples containing DNA and protein, then an instrument with a bandwidth of = 2nm should be used. In this case, there are demonstrative advantages in using a spectrophotometer with a bandwidth of 1nm. The spectra of a sample containing 36g/mL DNA and 500 g/mL serum albumin are shown in Figure 4.


It is clear from these data that the A260/A280 ratios of the sample containing both DNA and protein measured in the 5nm bandpass instrument (1.11) and in the 1nm bandpass instrument (1.02) are not equivalent. While both ratios are significantly less than the value of 1.8 expected for pure DNA, this was a sample heavily weighted with protein (~93% by weight). A more interesting question is the lowest level of protein in a sample that can be determined by t
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Evaluation of FlashPlate in a Helicase Assay
2. Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard
3. Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography
4. Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
5. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
6. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
7. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
8. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
9. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
10. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
11. Analysis of urine and seawater samples by ultrasonic nebulization with a high resolution ICP spectrometer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: EFFECTS INSTRUMENT RESOLUTION ASSESSMENT DNA PURITY

(Date:10/22/2014)... Shimadzu Scientific Instruments introduces its Open Solution ... pain management and clinical markets. The software increases the ... allowing users to highlight and review results that are ... analysts to filter results by group or based on ... the need to sift through analytes of no interest. ...
(Date:10/22/2014)... October 22, 2014 Grace Century, a ... healthcare projects, announces the addition of Dr. Yousef ... its advisory team. Dr. Siddiqui will provide further healthcare ... A graduate of University College Medical School ... in medicine in 2001. With further certification as a ...
(Date:10/22/2014)... - iCo Therapeutics ("iCo" or "the Company") (TSX-V: ICO) ... Oral Amphotericin B program.  The company recently announced ... work involving samples from HIV/AIDS patients exposed to ... clinical studies and regulatory filings to move forward ... approximately $700,000 of funding and technological advice from ...
(Date:10/20/2014)... 2014 The report “Pharmacy Automation ... Packaging and Labeling Systems, Table-top Counters) by End-user ... Forecasts & Trends to 2019” analyzes and studies ... North America, Europe, Asia-Pacific, and the Rest of ... and 30 figures spread through 300 pages and ...
Breaking Biology Technology:Shimadzu Releases QuantAnalytics Open Access LCMS Software Package for Clinical Applications 2Dr. Yousef Siddiqui joins the Grace Century Advisory Team 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 3Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 2Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 3Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 4
... - Lorus Therapeutics Inc. (TSX:,LOR, AMEX: LRP) ... in the research and development of pharmaceutical ... today announced that the,Corporation has received the ... with Lorus, corporate reorganization completed on July ...
... July 16 BioWa, Inc. and,Medarex, Inc. ... they have entered into a,license agreement which ... to,BioWa,s COMPLEGENT(TM) Technology for enhancing the complement-dependent,cytotoxicity ... The license grants Medarex non-exclusive rights to ...
... 16 U.S. Genomics today announced,the award of ... of Homeland,Security (DHS) Science and Technology Directorate. The ... (BAND) program will,enable U.S. Genomics to continue development, ... for the detection of airborne,pathogens using single molecule ...
Cached Biology Technology:Lorus Therapeutics Receives $600 Thousand From Escrow Account 2Lorus Therapeutics Receives $600 Thousand From Escrow Account 3BioWa and Medarex Announce License of BioWa's COMPLEGENT(TM) Technology 2BioWa and Medarex Announce License of BioWa's COMPLEGENT(TM) Technology 3BioWa and Medarex Announce License of BioWa's COMPLEGENT(TM) Technology 4U.S. Genomics Awarded $9.1 Million Contract for Development of Advanced Biosensor by the U.S. Department of Homeland Security 2
(Date:10/16/2014)... new synthesis of recent research findings to inform their ... two states. , The Ecology and Management of ... Synthesis of the Relevant Biophysical Science and Implications for ... the U.S. Forest Service,s Pacific Northwest Research Station, is ... a synthesis of the large body of scientific information ...
(Date:10/16/2014)... tract infections and worldwide claims the lives of ... Ghent University have succeeded in developing a promising ... , Xavier Saelens (VIB/UGent): "We discovered a ... the development of a novel approach to vaccination ... numerous small children and elderly people." , ...
(Date:10/15/2014)... 2014 Sandata Technologies, LLC, an industry ... care, today announced it released a case study ... Visit Verification™ Solution (EVV™)  for Quality Care Services, ... company founded in 1996 and has five locations ... The study details the challenges facing Quality ...
Breaking Biology News(10 mins):New report synthesizes best available science on management of moist mixed-conifer forests 2New perspectives for development of an RSV vaccine 2Sandata Announces Case Study with Quality Care Services, Inc. 2
... flowers have evolved particular colours, shapes and scents ... using artificial flowers and high-speed video, researchers have ... hummingbirds and the flowers they pollinate. The study, ... Functional Ecology , is the first to measure ...
... grey seal mums adopt risky tactics when it comes to the ... a real advantage, according to scientists. Researchers from Durham University, ... colonies in Scotland, found that some seal mothers are flexible in ... their actions, whilst other play it safe and steady. ...
... Calif.) When your neighbor asks to borrow a cup of ... of the give and take that characterize your longstanding relationship? Or ... of sugar you borrowed last week, or may need to borrow ... anthropology at UC Santa Barbara, and a junior research fellow at ...
Cached Biology News:High-speed video and artificial flowers shed light on mysteries of hummingbird-pollinated flowers 2Seals gamble with their pups' futures 2Seals gamble with their pups' futures 3UCSB anthropologist studies reciprocity among chimpanzees and bonobos 2UCSB anthropologist studies reciprocity among chimpanzees and bonobos 3