Navigation Links
EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY


The spectra in Figure 3 have been offset by 0.1A in order to show the enhanced details in the spectrum of serum albumin measured in the 1nm bandwidth instrument. With enhanced resolution, the contributions from phenylalanine, tyrosine, and tryptophan become more obvious. These residues absorb between 260 and 280nm. However, as was the case for the sample of pure DNA, there was little difference in the measured absorbance (~0.6) and wavelength maximum (~278nm) of the protein sample in the two instruments.

A much different situation applies when DNA purity is assessed. In this situation, the instrument must discriminate between DNA and likely contaminants. These contaminants include protein, chloroform, phenol. Contamination by protein is usually indicated by a decrease in the A260/A280 ratio. Contamination by an extraction solvent such as phenol or chloroform is indicated by increased absorbance at 320nm. If the criterion for acceptable resolution of instrument bandwidth ten-fold less than peak wavelength difference (280-260nm = 20nm) is applied to samples containing DNA and protein, then an instrument with a bandwidth of = 2nm should be used. In this case, there are demonstrative advantages in using a spectrophotometer with a bandwidth of 1nm. The spectra of a sample containing 36g/mL DNA and 500 g/mL serum albumin are shown in Figure 4.


It is clear from these data that the A260/A280 ratios of the sample containing both DNA and protein measured in the 5nm bandpass instrument (1.11) and in the 1nm bandpass instrument (1.02) are not equivalent. While both ratios are significantly less than the value of 1.8 expected for pure DNA, this was a sample heavily weighted with protein (~93% by weight). A more interesting question is the lowest level of protein in a sample that can be determined by t
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Evaluation of FlashPlate in a Helicase Assay
2. Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard
3. Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography
4. Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
5. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
6. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
7. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
8. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
9. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
10. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
11. Analysis of urine and seawater samples by ultrasonic nebulization with a high resolution ICP spectrometer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: EFFECTS INSTRUMENT RESOLUTION ASSESSMENT DNA PURITY

(Date:7/24/2014)... 24, 2014 SRI International has been awarded ... Institute of Allergy and Infectious Diseases (NIAID), part of ... of potential therapies for HIV infection and AIDS. The ... and AIDS and the complications and opportunistic infections associated ... sexual transmission of HIV. According to ...
(Date:7/24/2014)... 2014 Deep Research Report on Global ... and in-depth research report on Potassium Sulphate ... information, including its definition, classification, application, and industry ... the international market analysis, including China’s domestic market ... environment & economic situation analysis, the influence of ...
(Date:7/24/2014)... July 24, 2014 1000 balances – ... input on customers’ weighing needs and expertly narrows down ... of balances, scales or even terminals. , First, ... smallest weight (or select readability instead) and define the ... additional selection criteria narrows down the list to models ...
(Date:7/24/2014)... July 24, 2014  Spherix Incorporated (SPEX) -- an ... monetization of intellectual property, today announced that the Company ... June 30, 2014 on Monday, August 18, 2014 for ... a.m. ET on Monday, August 18, 2014 to discuss ... the company,s CEO, Anthony Hayes . ...
Breaking Biology Technology:National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 2National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 2Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 4Spherix Schedules Shareholder Update Call 2
... , , ... ("the Company"), a late-stage drug development company specialized in oncology and endocrinology, today reported financial ... . , , , ... - January 25, 2010: Updated results of a Phase 2 study related to the use, ...
... steel and triple that of the currently best synthetic ... one has thus far succeeded in producing the super ... and elastic fibers from the spider silk proteins stored ... the Technische Universitaet Muenchen (TUM) and the University of ...
... , May 12 /PRNewswire-Asia-FirstCall/ -- Yongye International,Inc. (Nasdaq: ... of Shengmingsu brand plant and animal nutrient,products in the People,s ... quarter ended March 31, 2010 . , , ... 100.5% to $24.9 million from the first quarter of, ...
Cached Biology Technology:Aeterna Zentaris Reports First Quarter 2010 Financial and Operating Results 2Aeterna Zentaris Reports First Quarter 2010 Financial and Operating Results 3Aeterna Zentaris Reports First Quarter 2010 Financial and Operating Results 4Aeterna Zentaris Reports First Quarter 2010 Financial and Operating Results 5Aeterna Zentaris Reports First Quarter 2010 Financial and Operating Results 6Aeterna Zentaris Reports First Quarter 2010 Financial and Operating Results 7Aeterna Zentaris Reports First Quarter 2010 Financial and Operating Results 8Investigating how spiders spin their silk, researchers unravel a key step 2Investigating how spiders spin their silk, researchers unravel a key step 3Yongye International Announces First Quarter 2010 Results 2Yongye International Announces First Quarter 2010 Results 3Yongye International Announces First Quarter 2010 Results 4Yongye International Announces First Quarter 2010 Results 5Yongye International Announces First Quarter 2010 Results 6Yongye International Announces First Quarter 2010 Results 7Yongye International Announces First Quarter 2010 Results 8Yongye International Announces First Quarter 2010 Results 9Yongye International Announces First Quarter 2010 Results 10Yongye International Announces First Quarter 2010 Results 11Yongye International Announces First Quarter 2010 Results 12Yongye International Announces First Quarter 2010 Results 13Yongye International Announces First Quarter 2010 Results 14
(Date:7/24/2014)... A sake brewery has its own microbial terroir, meaning ... resemble those found in the product, creating the final ... the journal Applied and Environmental Microbiology . This ... census of a sake brewery. , Many sake ... author David A. Mills of the University of California, ...
(Date:7/24/2014)... College of Medicine of Yeshiva University have received ... Parkinson,s Research to translate a basic science finding into ... will test chemical compounds on a newly identified target ... on an underlying cause of the disease. , ... their symptoms, we are eager to stop or even ...
(Date:7/24/2014)... counterparts in humans, affect the connections between nerve cells ... hippocampus, an area of the brain that plays a ... memories. The results of the study have been published ... depends on the active communication between nerve cells, known ... into a dense network where they constantly relay signals ...
Breaking Biology News(10 mins):The microbes make the sake brewery 2Albert Einstein College of Medicine receives grant from the Michael J. Fox Foundation to fund drug discovery project targeting Parkinson's 2Albert Einstein College of Medicine receives grant from the Michael J. Fox Foundation to fund drug discovery project targeting Parkinson's 3A protein couple controls flow of information into the brain's memory center 2A protein couple controls flow of information into the brain's memory center 3A protein couple controls flow of information into the brain's memory center 4
... found that a protein that initiates a "quality control ... those cells damaged during duplication. This knowledge represents a ... appear in the current issue of Science. , The ... which works as a "quality control inspector." As normal ...
... years of remotely sensed imagery from the 1950s to 2002 ... more than 10,000 ponds in Alaska. The analysis, by University ... Journal of Geophysical Research, indicates that these landscape-level changes in ... and may have profound effects on climate and wildlife. , ...
... Chris Austin, assistant curator of herpetology at LSU's Museum ... biological sciences, recently discovered a new species of lizard ... along with colleague Indraneil Das from the Institute of ... will publish their findings and photos of the new ...
Cached Biology News:Mayo discovers protein as potential tactic to prevent tumors 2Shrinking ponds signal warmer, dryer Alaska 2LSU professor discovers new species 2
... GCpro Taq DNA Polymerase is a ... advantages for all PCR applications. GCpro Taq ... clone expressed in E. coli, containing the ... The enzyme includes a highly processive 5'-3' ...
Ps-Aeruginosa (IgG) -Ab EIA Sample Size: 100 l...
...
Automated -80° C sample library for microplates and cryotube racks in SBS format; for integration into automated processes....
Biology Products: