Navigation Links
EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY

ation of DNA purity. This communication addresses the effects of instrument bandwidth on spectrophotometric determination of DNA concentration and purity.


MATERIALS AND METHODS
Spectrophotometers used in this study were the Shimadzu models UV-2401PC (selectable bandwidth to 0.1nm), UV-1700 (fixed bandwidth, 1nm), and the UV-1240 (fixed bandwidth ,5nm). Calf thymus DNA and Bovine serum albumin were obtained from Sigma Chemical Company, St. Louis, MO. All other reagents were obtained from commercial sources and were of the highest available purity. Samples were prepared in 5mM KH2PO4 containing 145mM NaCl, pH 7.48. Semi-micro, far-UV quartz cells (~1.5mL) were used for all measurements.


RESULTS AND DISCUSSION
An example of the spectrophotometric determination of DNA is illustrated in Figure 1. In this figure, a sample of approximately 73 g/mL calf thymus DNA in physiological buffer was measured using a Shimadzu UV-2401 spectrophotometer with an instrument bandwidth of 1.0nm. As illustrated in Figure 1, this sample of pure DNA has an absorbance maximum (1.47) and minimum (0.62) at approximately 260 and 230nm, respectively. The width at half-height of this spectrum is approximately 43 nm.The spectrum of this same DNA sample was measured using instruments of 1 and 5nm fixed-instrument bandwidth. These data are shown in Figure 2.



The data in Figure 2 indicate that there is very little difference in the spectrum of pure, double-stranded DNA measured using spectrophotometers of 1 and 5nm instrument bandwidth. The absorbance of the sample at 260nm and the A260/A280 ratios are comparable and well within experimental error. A similar comparison of spectra of 1mg/mL Bovine serum albumin is shown in Figure 3.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Evaluation of FlashPlate in a Helicase Assay
2. Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard
3. Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography
4. Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
5. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
6. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
7. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
8. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
9. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
10. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
11. Analysis of urine and seawater samples by ultrasonic nebulization with a high resolution ICP spectrometer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: EFFECTS INSTRUMENT RESOLUTION ASSESSMENT DNA PURITY

(Date:1/22/2015)... Protocol Networks brings independent technology consulting to the ... consultants, Protocol Networks has recently announced its expansion into ... his company has attracted several clients in The Constitution ... as others. With the success of these engagements, Rogers ...
(Date:1/22/2015)... Diego, California (PRWEB) January 22, 2015 ... Promotional Offer to their comprehensive portfolio of Eppendorf ... allows customers to purchase a Centrifuge 5424/5424 R and ... Bundle of either Eppendorf Research plus or Eppendorf Reference ...
(Date:1/22/2015)... Dr. Greg Leyer of UAS Labs recently ... Supplements Pre-Conference seminar on probiotics in San Diego, CA. ... education conference for health care professionals. This year’s pre-conference seminar ... probiotics in health. Dr. Leyer spoke about the emerging topics ...
(Date:1/22/2015)... Selexis SA, a serial innovation company with proven ... for drug discovery to commercial manufacturing, announced today all ... Sequencing (NGS) data packages. The NGS analysis of ... the integrity of the gene, validation of the left ...
Breaking Biology Technology:Protocol Networks Brings Independent Technology Consulting to the Constitution State 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2
... 10:00 CET, AMSTERDAM, February 20 Amsterdam Molecular,Therapeutics (Euronext: AMT), ... full year 2007 results., Highlights - ... Cash & cash equivalents of EUR 51.3 million at June 30, ... AMT 011 - Positive results of Phase I/II in LPL ...
... new technologies launched onboard a U.S. Naval Academy satellite ... space. One technology is a sensor that can check ... "film" that can control heat. , These technologies were ... Md.; NASAs Ames Research Center, Moffett Field, Calif.; and ...
... a collaboration between researchers at the National Institute of ... of Kentucky potentially offers a low-cost way to mass ... sheets for a wide range of applications including flexible ... for field diagnostics. , In a paper posted this ...
Cached Biology Technology:Amsterdam Molecular Therapeutics Reports Full Year Results 2007 2Amsterdam Molecular Therapeutics Reports Full Year Results 2007 3Amsterdam Molecular Therapeutics Reports Full Year Results 2007 4Amsterdam Molecular Therapeutics Reports Full Year Results 2007 5Amsterdam Molecular Therapeutics Reports Full Year Results 2007 6Amsterdam Molecular Therapeutics Reports Full Year Results 2007 7NASA MidSTAR-1 successful technologies may be revolutionary 2NASA MidSTAR-1 successful technologies may be revolutionary 3NASA MidSTAR-1 successful technologies may be revolutionary 4Directed self-ordering of organic molecules for electronic devices 2
(Date:1/22/2015)... Wis. , Jan. 15, 2015  BellBrook Labs, ... drug discovery, announced the launch of a TR-FRET ... the company,s Transcreener UDP Assay, a high throughput ... The new assay will allow for sensitive detection ...
(Date:1/22/2015)... 22, 2015 , Europe,s ... The European Patent Office to present a video retrospective of ... to be featured: Christofer Toumazou and Sophie Wilson ... and winners of the Award   Starting on 22 ...
(Date:12/22/2014)... , Dec. 22, 2014  NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market, announces ... Apollo Robbins for the 2015 International CES debut of ... Robbins will be at the NXT-ID booth January 6th ...
Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4
... peculiarities with the DNA of just about every other plant ... as transposons, or "jumping genes," which have the ability to ... cell. An astounding 50% of human DNA ... former transposons that were active thousands to millions of years ...
... (Sept. 6, 2011) The American Physiological ... and the ENaC/Degenerin Family of Ion Channels, being held ... Grove, Calif. This scientific conference explores the connection ... growing global health concern. The five-day program ...
... surgeons are working together to improve the treatment of ... bone plates in the skull to fuse too soon. ... remove portions of the fused skull bones, and in ... -- requiring additional surgeries. Researchers in the Atlanta-based ...
Cached Biology News:A 'jumping gene's' preferred targets may influence genome evolution 2A 'jumping gene's' preferred targets may influence genome evolution 3Highlights of upcoming conference on aldosterone & the ENaC/Degenerin family of ion channels 2Developing technologies to improve the treatment of craniosynostosis in children 2Developing technologies to improve the treatment of craniosynostosis in children 3Developing technologies to improve the treatment of craniosynostosis in children 4
assays for phosphatase, lipase, nucleoside triphosphatases (ATPase, GTPase etc). HTS and follow up studies for phosphatase inhibitors. Quantitation of phosphate content in protein, lipids, and DNA....
One-step, microplate or cuvet, turbidometric, linear detection range 0.096 mg/dL to 11.5 mg/dL. Procedure: 5 min....
Homo sapiens 4-aminobutyrate aminotransferase Antigen: Recombinant Protein...
Request Info...
Biology Products: