Navigation Links
EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY


INTRODUCTION
The traditional method for quantification and assessment of purity of DNA samples is spectrophotometric measurement at 260 and 280nm (1). It is generally accepted that a sample of pure, double-stranded DNA of 50 g/mL will have an absorbance at 260nm of 1.0 and that the ratio of absorbance at 260 and 280nm of this sample will be greater than 1.8 (2). If a sample has an A260 / A280 of less than 1.8, it is usually considered to be contaminated by protein. In such measurements, there is frequently little consideration given to the resolution or bandwidth of the spectrophotometer used for the measurements.

Instrument bandwidth is generally defined as the full width at half height of an absorbance band of a reference material that possesses a natural bandwidth less than or equal to the instrument bandwidth. The ideal reference material would be one with many absorbance bands, each having an infinitely small natural bandwidth. For this reason, the atomic lines of elements such as Hg are used as reference materials for determining instrument bandwidth.

Each sample will have a natural or Spectral Bandwidth and, thus, the ability of an instrument to accurately quantify components in a mixture will depend upon several factors, including natural bandwidths of the components and the instrument bandwidth. A widely accepted practice is to use a spectrophotometer with an instrument bandwidth of one tenth of the natural bandwidth of the analyte to be measured, 43 nm in the case of DNA. Thus, following the accepted practice, a spectrophotometer with an instrument bandwidth of = 4.3nm should be used to measure such samples. In the past few years there has been an increasing trend within life sciences toward the use of spectrophotometers with instrument bandwidths of 5nm or greater for quantification of DNA and for estim
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Evaluation of FlashPlate in a Helicase Assay
2. Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard
3. Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography
4. Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
5. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
6. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
7. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
8. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
9. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
10. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
11. Analysis of urine and seawater samples by ultrasonic nebulization with a high resolution ICP spectrometer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: EFFECTS INSTRUMENT RESOLUTION ASSESSMENT DNA PURITY

(Date:7/10/2014)... CA (PRWEB) July 10, 2014 Senior ... institutions and professional associations, addressed the challenges of “Reducing ... Medical Device Industries” through strategic sourcing. Describing the partnership ... with the Supply Chain Management Institute ( SCMI) ... event on June 24 at the Kroc Institute of ...
(Date:7/10/2014)... , July 10, 2014 On ... Dmitry Medvedev , presented Russia,s ... BIOCAD . The Company is developing a unique project ... project BIOCAD develops a number of innovative drugs based ... diseases. The ceremony took place at the International Exhibition ...
(Date:7/10/2014)... , July 10, 2014 /PRNewswire-iReach/ -- Tute ... interpretation, today announced an agreement with Lineagen, ... for Lineagen,s NextStep Dx PLUS.  Lineagen, ... and neurological disorders, currently offers FirstStep Dx ... testing service used by healthcare providers as ...
(Date:7/10/2014)... July 10, 2014   Ceres, Inc . (Nasdaq: ... company, announced today financial results for the three months ... its business. Ceres reported that the company ... performance this growing season in Brazil, which concluded in ... company,s sorghum evaluation areas for part of the growing ...
Breaking Biology Technology:Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9
... witnessed a biotech meltdown during the first quarter of 2005 ... will take a look at the larger cap life science ... for the pharmaceutical sector. You may have noticed headlines at ... decision to pull its Cox-2 inhibitor (Bextra) off the market. ...
... series that will follow entrepreneurs through the trials of starting ... finalists in the Governor's Business Plan Contest. Surgeons have little ... procedures. For Milwaukee-area general surgeon Michael Phillips, frustration with having ... out during cancer surgeries led to the creation of the ...
... me that if you couldn't say anything nice, don't say ... ,Occasionally something so egregious happens that it must be addressed. ... products. , ,What's got my steam vents working overtime is ... in a single pass." Oh my. What kind of business ...
Cached Biology Technology:Midwest large-cap life-science sector is solid in first quarter 2Vector Surgical hopes to turn frustration into faster surgeries 2Vector Surgical hopes to turn frustration into faster surgeries 3Double your productivity! Hype that damages technologists' reputations 2Double your productivity! Hype that damages technologists' reputations 3
(Date:7/11/2014)... normal circumstances, astrocytes participate in normal physiological ... exhibit therapeutic and repairing effects on brain ... found that nerve cells differentiated from adipose-derived ... viability, which produces influences on subsequent studies ... Hospital, Hebei United University, China demonstrated that ...
(Date:7/10/2014)... Santa Fe, Albuquerque, and other major cities in New ... with treated municipal wastewater rather than precious potable water ... than 40% of all golf courses receive treated effluent. ... , Additionally, golf courses and homeowners alike fertilize ... in fertilizer is nitrate. A New Mexico State University ...
(Date:7/10/2014)...  Unisys Corporation,s (NYSE: UIS ) subsidiary in ... announced that it has been selected by the Dutch Custodial ... Basic Provision Biometrics solution for penitentiaries across The ... with a solution based on its open standards-based LEIDA ... contract is up to seven years, with the new system ...
Breaking Biology News(10 mins):'Tailored' water -- the latest in lawn care 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 3
... in salads are chock-full of important vitamins and nutrients, ... type and amount of salad dressing, a Purdue University ... subjects salads topped off with saturated, monounsaturated and polyunsaturated ... fat-soluble carotenoids ╨ compounds such as lutein, lycopene, beta-carotene ...
... (June 19, 2012) Using data from its unique online ... to the finding of six novel genetic associations for early ... study now published online in the journal PLoS Genetics ... of new genetic discoveries. Nearly 90 percent of ...
... 2012 Digicel Pacific Limited, a mobile network operator across ... it is deploying biometric enabled payment systems into stores in ... more convenient way to send money home to the Pacific. ... home to register just once and record their fingerprint as ...
Cached Biology News:Study: No-fat, low-fat dressings don't get most nutrients out of salads 223andMe contributes to genetic discoveries related to male pattern baldness 223andMe contributes to genetic discoveries related to male pattern baldness 3Digicel Launches World's First Biometric Identification System For International Money Transfers 2Digicel Launches World's First Biometric Identification System For International Money Transfers 3
IHC detection kit for PCNA in cells and tissues...
... Immunogen: Synthetic peptide derived from ... WNT1 protein. Specificity: Reacts with ... Reactivity: Human Mouse (positive controls: ... cell lysates and mouse Mouse-1 cell ...
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2 origin for high-copy maintenance....
... [125I] Direct SPA Screening Assay, ... reagents that eliminate lengthy sample extraction ... (optional use).Range: 1.32-84.28 ng/ml (4-256 pmol/ml).Sensitivity: ... at 2-8 C. Category: Drug Screening ...
Biology Products: