( Intro...)E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor

Introduction
This Application Report is part of a series documenting culture growth in the BioFlo 110. With appropriate vessels and control modules, the BioFlo 110 can be used to efficiently grow mammalian cells, plant cells, insect cells, yeast, and bacteria.

For this report, a standard 7.5L BioFlo 110 Advanced Fermentation Kit, NBS Catalog Number M1273-1125 was first used for an E. coli fed-batch fermentation.

Next, a BioFlo 110 Gas-Mix Controller, M1273-3104, was added, and the fermentation repeated with oxygen supplementation of the sparge gas. This second culture, described in the APPENDIX, achieved a very high dry cell weight of 57.2 g/L. Neither run was fully optimized, but the descriptions of procedures and materials as well as the data discussion will be useful to operators of similar fermentors.

The Fermentor

Vessel
The BioFlo 110 Advanced Fermentation Kit used for this work was equipped with a heatblanketed 7.5 L fermentation vessel with nominal 5.7 L working volume. All BioFlo 110 fermentation vessels are configured with a 4-baffle stainless-steel insert, dual Rushton agitation impellers, and a high-speed, direct-drive agitation system with mechanical face-seal. Dissolved oxygen and pH probes (Mettler- Toledo) are also included with these fermentors. We expect similar results from BioFlo 110 fermentation vessels from 1 through 14 L in volume, and in both heat blanket and water jacket configurations.

Control System
The four control modules included with the Advanced Fermentation Kit were used for the first run. (See Chart)

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