Navigation Links
Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

/M value, so the ratios appear automatically in the group table. If, instead, the reduction is set to P value or M value, that set of values would appear in the group table.

If you wish to list both the firefly and renilla values in the group table, as well as the ratios, you can create additional columns and type in the desired formulas. Those formulas are summarized in the table below.


An example of a group table containing the 3 values (and other information) is shown in Figure 4. In this example the purified enzyme preparations were diluted in tandem, so the ratios are predictably the same for all of the samples (except the very lowest concentrations). In real cell-based experiments, the ratios would differ.


SUMMARY
The Lmax microplate luminometer is a sensitive and easy-to-use instrument for measuring firefly and renilla luciferase activity in a microplate format. The dual sequential measurements can be automated, and SOFTmax PRO for Lmax offers automatic calculations of the ratios of the two activities as well as additional powerful data analysis capability.


REFERENCES

1. www.promega.com

2. DeLuca, M.A. and W.D. McElroy, (1978) in Meth. Enzymol. vol 53:, p.3

3. Matthews, J.C. et al. (1977) Purification and properties of Renilla reniformis luciferase. Biochemistry, 16:85.


By default, SOFTmax PRO brings only the single set of reduced data values into the group table. If you want additional data listed in the group table (e.g. more than one reading or additional secondary calculations), you must create new columns and specify the desired values by typing in custom formulas. For details, see your SOFTmax PRO Users Manual.




Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:12/19/2014)... REDWOOD CITY, Calif. , Dec. 19, 2014 ... , USA) announced today that they have been ... Technologies 1,2 is a privately held company that ... processing of next generation sequencing (NGS) data for the ... into the Roche Sequencing Unit, and will continue to ...
(Date:12/19/2014)... Today, TiFiber, Inc. ... facility and future company headquarters in Fort Smith, ... materials that safely and effectively control dangerous microorganisms. ... its historic, strong support for manufacturing, but also ... edge, technology-based companies such as TiFiber. , ...
(Date:12/19/2014)... Boston, MA (PRWEB) December 18, 2014 ... software, announced the appointment of Siri Bryant as the ... the new Director of Core Implementations. These two new ... provide outstanding customer service by ensuring that iLab continues ... , “In the last four years, iLab has been ...
(Date:12/19/2014)...  Decision Resources Group finds that the ... moderately through 2023 as the aging population, increasing adoption ... biomaterials all spur procedure volumes. Growth will be particularly ... and India , where ... for a larger proportion of the population. ...
Breaking Biology Technology:Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3Technology Company TiFiber™ to Establish Production Facility in Fort Smith, Arkansas with Support from Local Investors 2Technology Company TiFiber™ to Establish Production Facility in Fort Smith, Arkansas with Support from Local Investors 3Technology Company TiFiber™ to Establish Production Facility in Fort Smith, Arkansas with Support from Local Investors 4iLab Solutions Announces Two New Leadership Positions to Meet the Needs of Its Growing Customer Base 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 3
... ... in Eighteen Platform and Poster Sessions. Its EPA-approved technologies will be widely highlighted during ... 24-27 in Monterey, California. , ... Los Angeles, CA (Vocus) May 1, 2010 -- The Adventus Group, a ...
... FREISING-WEIHENSTEPHAN, Germany , April 30, 2010 ... proprietary PRS-110 Anticalin(R),compound, which targets the c-Met receptor, demonstrating ... model. The results provide an,indication that an Anticalin approach ... value in treating cancer. A broad body of,research has ...
... chemistry classes often start off with learning how to ... Mellon University chemistry professor David Yaron, seems to be, ... interesting, you may get to use it later." ... students to design and carry out experiments more like ...
Cached Biology Technology:ADVENTUS Bioremediation Technologies Highlighted at Battelle Symposium. 2ADVENTUS Bioremediation Technologies Highlighted at Battelle Symposium. 3ADVENTUS Bioremediation Technologies Highlighted at Battelle Symposium. 4Pieris Announces Positive Preclinical Data on c-Met Anticalin Drug Program 2Virtual lab wins prestigious prize awarded by Science 2Virtual lab wins prestigious prize awarded by Science 3Virtual lab wins prestigious prize awarded by Science 4
(Date:12/5/2014)... , Dec. 4, 2014  Tute Genomics, a leader ... million in Series A1 funding led by UK-based Eurovestech. ... participated in the investment round. "We are ... community adopts next-generation sequencing and seeks new approaches for ... Reid Robison , MD MBA, and CEO of Tute ...
(Date:12/3/2014)... 2014   Marvin Test Solutions , a ... for military, aerospace, and manufacturing organizations, today announced ... TS-900 PXI semiconductor test platform . The ... systems to customers at a fraction of the ... ATE. "Our semiconductor customers asked ...
(Date:11/21/2014)... November 18, 2014 According to ... by Systems (Video, RFID, Access Control, Intrusion Detection, Parking ... Banks, Government), Component Service Geography - Global Forecasts to ... is projected to be around $25 Billion in 2014 ... growing at a CAGR of 8.69%. Browse ...
Breaking Biology News(10 mins):Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3
... (April 23, 2012) The benefits of breast milk are well ... remains a mystery. A new study in the Journal ... of cancer-fighting TNF-related apoptosis inducing ligand (TRAIL) in human milk, ... , Researchers took samples of colostrum, the first ...
... Biology 2012, Dr. Blake Rasmussen and colleagues presented a ... vs. Whey Protein Ingestion on Muscle Protein Synthesis Following ... that a protein blend (combination of soy, whey and ... muscle. Specifically, the blend of proteins in this study ...
... Tampa, Fla. (April. 23, 2012) Researchers interested ... fat and high cholesterol (HFHC) diet on implanted fetal ... the HFHC diet elevated microglial activation and reduced neuronal ... inflammatory response in the central nervous system, they found ...
Cached Biology News:New study finds a protein combination is best to consume post-workout for building muscle 2Study finds 'Western diet' detrimental to fetal hippocampal tissue transplants 2
... DNA Engine Dyad thermal cycler is ... (PCR). The chassis accommodates a variety ... assemblies), which hold different types and ... graphical programming with point-and-click navigation. Peltier ...
MiniCycler thermal cycler with Hot Bonnet heated lid, holds 25x0.2ml tubes...
For demonstration of Helicobacter pylori...
... FLISP Assay with SR-101 Labeled Substrates ... • Accurately measure serine protease activation ... • B-Bridge's FLISP Kits utilize cell-permeable, ... (FLISPs) to measure chymotrypsin-like activity in ...
Biology Products: