Navigation Links
Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

Sup> directly under the read head.


EXAMPLE OF DUAL LUCIFERASE RESULTS OBTAINED ON THE LMAX
A SOFTmax PRO plate section containing raw dual luciferase data is shown in Figure 2. The instrument settings are displayed to the right, along with the time/ data stamp. In each well, there are 3 numbers: the top is the firefly luciferase value (P injector), the middle is the Renilla luciferase value (M injector), and the bottom number is the ratio of the two. (The ratio was selected in the Reduction dialog box in SOFTmax PRO, and the Display was set to show Raw + Reduced data.)


The two standard curves are shown in Figure 3. The signal from the renilla luciferase was approximately 10 times brighter than that of the firefly enzyme. The calculated limits of detection (amount producing a signal higher than 3 positive SD of the blank values above zero) were approximately 2 and 0.2 fg/well for the firefly and renilla enzymes respectively.


DUAL READ VALUES IN THE GROUP TABLE
Although the examples presented here are standard curves of firefly and renilla luciferase, the purpose of the dual assay is to ascertain the ratios of the two activities. The ratio can be calculated automatically in SOFTmax PRO if you specify the ratio as the desired calculation in the Reduction Dialog Box. If you also wish to display the individual values in the Group Table, this can be easily accomplished as described below.

When you create a group and assign sample locations in Template Editor, SOFTmax PRO automatically creates a group table to list the sample names and the corresponding data. The actual values which appear in the group table depend on which data calculation you have selected in the Reduction dialog box. In the example shown in Figure 2 above, the reduction is set to the ratio of P value
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:9/18/2014)... diseases that afflict skeletal growth through abnormalities in ... fetal stage and is caused by genetic mutations. ... factor receptor 3 (FGFR3) has been associated with ... a skeletal dysplasia that cause serious respiratory problems ... (ACH), which causes stunted growth and other complications ...
(Date:9/18/2014)... Scientists at USC have developed a new microscopy ... in living animals at higher-than-ever resolution. , Dubbed ... allows imaging resolutions that are an order of ... insights into the behavior of biomolecules at the ... Sept. 18 by Nature Communications , the ...
(Date:9/18/2014)... Silicon-valley Electronic Data Capture (EDC) provider ... , the latest addition to Clinovo’s CRO Partnership Program. ... (CRO) Program, Clinovo further expands its global reach to ... training, we are now able to build studies on ... can also rely on Clinovo to fully support the ...
(Date:9/18/2014)... Whitehouse Laboratories is pleased to announce that ... has acquired an additional 4,000 square feet of dedicated ... adjacent to its current package testing facility. The official ... Whitehouse Labs is in the process of upgrading the ... testing. The prime location enables Whitehouse Labs to immediately ...
Breaking Biology Technology:Researchers use iPS cells to show statin effects on diseased bone 2Researchers use iPS cells to show statin effects on diseased bone 3Scientists pioneer microscopy technique that yields fresh data on muscular dystrophy 2Digitaliza TXT Joins Clinovo’s CRO Partnership Program, Bringing Its Open Source EDC System, ClinCapture to Latin America 2Digitaliza TXT Joins Clinovo’s CRO Partnership Program, Bringing Its Open Source EDC System, ClinCapture to Latin America 3Whitehouse Labs Announces Package Testing Laboratory Expansion 2Whitehouse Labs Announces Package Testing Laboratory Expansion 3Whitehouse Labs Announces Package Testing Laboratory Expansion 4
... T Slyker, B Gillece-Castro, M Nguyen, L Sapp, MG Brubacher, and WB , ... Authors at The Association of Biomolecular Resource , Facilities (ABRF) ... Denver, Colorado , Abstract , ... for proteome , analysis. The quality and value of the ...
... Kilby, Roche Discovery Welwyn, 40 Broadwater Road, Welwyn , ... Syngenta, Jealotts Hill International Research Centre, Bracknell, Berkshire, , ... Abstract , ... running 2-D SDS-PAGE slab gels with preparative protein loads. The ...
... Contributed by Alan T. Bakalinsky Department of Food Science and Technology, , ... Abstract , ... compared to those of a standard laboratory ... findings that two of the wine strains are aneuploid , ...
Cached Biology Technology:Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A 2Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A 3Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A 4Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A 5Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A 6Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 2Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 3Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 4Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A 5Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae 2Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae 3Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae 4Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae 5Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae 6
(Date:9/18/2014)... manipulate spoken language is unique to humans. "The genetic ... of human evolution to make this possible are largely ... now have," says Wolfgang Enard, Professor of Anthropology and ... the molecular biological basis of language Enard has now ... latest study, undertaken in collaboration with scientists at several ...
(Date:9/18/2014)... Dogs generally seem to be cheerful, happy-go-lucky characters, so ... outlook on life. , In fact some dogs ... University of Sydney shows. , "This research is ... in dogs objectively and non-invasively. It offers researchers and ... and how that changes," said Dr Melissa Starling, from ...
(Date:9/18/2014)... that when people are too stressed they are often ... Brain Mind Institute (BMI) at EPFL have just highlighted ... chronic stress and the loss of social skills and ... a synaptic regulatory molecule in the brain. This was ... . , Carmen Sandi,s team went to look ...
Breaking Biology News(10 mins):Language evolution: Quicker on the uptake 2Language evolution: Quicker on the uptake 3Dogs can be pessimists too 2Dogs can be pessimists too 3How stress tears us apart 2
... case state-of-the-art energy efficient housing will be officially opened ... Nottingham. The house built by BASF, a major ... part of the Creative Energy Homes Project on University ... of Built Environment to stimulate sustainable design ideas and ...
... Heritage Foundation (CHF) announced that Leroy Hood ... Award. Jointly sponsored by the Pittsburgh Conference ... and CHF, this award recognizes outstanding individuals ... community, inspired achievement, promoted public understanding of ...
... Ala. The long-sought-after biological gateway that anthrax uses ... at the University of Alabama at Birmingham (UAB). ... a receptor that sits on the surface of certain ... exactly how the bacteria get inside cells to begin ...
Cached Biology News:Opening day for a home of the future 2Opening day for a home of the future 3Leroy Hood to receive 2008 Pittcon Heritage Award 2Leroy Hood to receive 2008 Pittcon Heritage Award 3Anthrax cellular entry point uncovered 2
... 45° Fixed Angle rotor for ... CL2, Centra CL3/CL3R, HN-SII or ... style rotor accepts 15 mL ... disposable conical tubes as well ...
Rotor for four tube-loaded buckets; ma RCF 2550g; sold without buckets...
45° Fixed Angle Rotor for use in the IEC Centra CL2, Centra CL3/CL3R, HN-SII, and the Centra-MP4/MP4R....
A camera based compact unit for high speed scanning of 2D barcoded tubes in a cluster rack. This reader has been designed to read both 96 and 48 tube rack configurations and be compatible with common...
Biology Products: