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Drive Long-Term Silencing with CMV Vectors

primary antibodies and a fluorescein conjugated secondary antibody and the resulting reduction in protein expression was examined by fluorescence microscopy. The cells were mounted with DAPI to stain nuclei (Blue). Images were digitally captured and quantified using Metamorph software.


The pSilencer 4.1-CMV siRNA Expression Vectors are provided with 1) linearized and purified vector ready for ligation; 2) a DNA insert encoding a GAPDH-specific siRNA; 3) a circular, negative control pSilencer vector that expresses a scrambled control siRNA; and 4) 1X DNA Annealing Solution.


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Ordering Information
Cat# Product Name Size 5775 pSilencer 4.1-CMV puro 20 rxns 5777 pSilencer 4.1-CMV hygro 20 rxns 5779 pSilencer 4.1-CMV neo 20 rxns 5790 pSilencer adeno 1.0-CMV System 5 virus preparations
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Preparation Note: Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948). Quality Clarifier: Contains lipase Unit Definition: One unit will hydrolyze 1.0 μmole of p-ni...
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