Navigation Links
Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Figure 2. Workflow of the double-affinity purification
The GST-HIS fusion protein was first purified by affinity chromatography via the GST-tag. The GST-HIS double tag was enzymatically removed from the target protein, and eliminated by an IMAC purification step via its poly-His moiety. The purified, non-tagged target protein was recovered in the IMAC flow-through.

Figure 3. Recovery of the purified, non-tagged target protein
The purified, non-tagged target protein was recovered in the flow-through after the IMAC purification step (analysis by SDS-PAGE and Coomassie blue staining)


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
5. Easy Cosmid DNA Purification with StrataPrep Kits
6. MinElute PCR Purification Kit
7. QIAquick PCR Purification Kit
8. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
9. Ultra-Fast Purification of Small Nucleic Acids
10. RNAClean - RNA & cDNA in vitro Reaction Purification
11. RNAPrep - Total RNA Isolation Purification
Post Your Comments:
(Date:1/15/2014)... , Jan. 15, 2014  Bill Jacobs Automotive, a group of ... teaming up with the Heartland Blood Center and offering free ... The drive takes place Saturday, Jan. 18 at the Bill ... p.m. (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  The ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 Cynvenio, a ... management through the genomic analysis of tumor cells in ... Finder™, a web-based cancer decision support application powered by ... with Cynvenio’s ClearID™ genomic test, Therapy Finder will provide ...
(Date:1/15/2014)... A study has been launched to test whether ... could help to tackle the problem of obesity.  This unique ... surgery based in Stowmarket) and academics at University Campus Suffolk ... technology, which is inspired by equipment used to collect data ...
(Date:1/15/2014)... 2014 DTS Language Services, Inc . ... Portal for Life Science organizations who need document translations. ... matter of their documents in advance with a selection of ... of translations, often a critical factor in clinical and scientific ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4DTS Improves Efficiency for Life Science Document Translations 2
... Pharmaceuticals, Inc. (Nasdaq: QCOR ) today announced that ... S&P SmallCap 600 Index effective after the close of the ... "Our inclusion in the S&P SmallCap 600 Index reflects our ... within the investment community as we continue our efforts to ...
... Barbara, Calif.) An important milestone toward the realization of ... level of the quantum control of light, were accomplished by ... China and Japan. The study, published in the Feb. ... involved scientists from Zhejiang University, China, and NEC Corporation, Japan. ...
... 15, 2011 Gen-Probe Incorporated (Nasdaq: GPRO ) ... year 2010, highlighted by record non-GAAP earnings per share (EPS) ... prior year period. "In the fourth quarter of ... high in earnings per share despite macroeconomic headwinds and unusually ...
Cached Biology Technology:Questcor Pharmaceuticals to Be Added to S&P SmallCap 600 Index 2International team of scientists says it's high 'NOON' for microwave photons 2Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 2Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 3Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 4Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 5Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 6Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 7Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 8Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 9Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 10Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 11Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 12Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 13Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 14Gen-Probe Reports Financial Results for the Fourth Quarter and Full Year 2010 15
(Date:4/17/2014)... is available in German . ... the protection money demanded of him, he can expect his ... however, as fear of the consequences is enough to make ... parasitic birds, which lay their eggs in other birds, nests. ... brood parasites take their revenge by destroying the entire nest. ...
(Date:4/17/2014)... such as heart disease, epilepsy and pain, making them ... is still much scientists do not know about the ... into the structure of sodium channels and, specifically, one ... responsible for ,fine-tuning, the activity of the channel. The ... the Journal of Biological Chemistry . , Nerves ...
(Date:4/17/2014)... is the process whereby the genetic information of ... as proteins, which have numerous different functions in ... important intermediary during gene expression, by relating the ... involved in manufacturing proteins. , By examining ... present in an organism at a given time, ...
Breaking Biology News(10 mins):Fear of the cuckoo mafia 2Fear of the cuckoo mafia 3Structure of sodium channels different than previously believed 2Rapid and accurate mRNA detection in plant tissues 2
... businesses in Munich, Germany and Ann Arbor, Michigan released ... the National Institute on Aging, National Institutes of Health, ... (ES) cells. , In their work they first identified ... qRT-PCR methods. Then they employed extensive DNA promoter analysis ...
... for species exploration and classification, has been largely ignored ... International Institute for Species Exploration at Arizona State University ... spark a renaissance in taxonomy through a transdisciplinary fusion ... an entomologist and ASU vice president and dean of ...
... plants from a sudden freeze that occurred across the Eastern ... made worse because it had been preceded by two weeks ... March 2008 issue of BioScience. The authors of the report, ... Laboratory and collaborators at NASA, the University of Missouri, and ...
Cached Biology News:Species explorers ask: What's on Your planet? 2Species explorers ask: What's on Your planet? 3Will global warming increase plant frost damage? 2
... in the submicron range are part of ... and control of particle size is important ... can reliably detect the component populations in ... Particle Size Analyzer uses Photon Correlation Spectroscopy ...
...
... a dedicated system for SFC and SFE ... of HPLC instrumentation. JASCO has recently added ... of the system: ,- The PU-1580-CO2 delivery ... procedures using carbon dioxide as the mobile ...
... ( Abpromise for all tested ... Synthetic peptide: IGPEVPDDRDFEPS conjugated to KLH, ... Human Decorin (fragment 36-49 of the ... Entrez GeneID: 1634 ...
Biology Products: