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Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Figure 2. Workflow of the double-affinity purification
The GST-HIS fusion protein was first purified by affinity chromatography via the GST-tag. The GST-HIS double tag was enzymatically removed from the target protein, and eliminated by an IMAC purification step via its poly-His moiety. The purified, non-tagged target protein was recovered in the IMAC flow-through.

Figure 3. Recovery of the purified, non-tagged target protein
The purified, non-tagged target protein was recovered in the flow-through after the IMAC purification step (analysis by SDS-PAGE and Coomassie blue staining)


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