Navigation Links
Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH

Mike Benway, Larry Simonson and John Ung
Caliper Life Sciences Inc, Applications Laboratory, Hopkinton, Massachusetts, USA

Introduction

"Assay-Ready" plates prepared from compound or library stock plates in real time greatly accelerates the High Throughput Screening (HTS) process. The ability to transfer and dilute 0.5μL volumes is a prerequisite for this function. Here we present preliminary data on 0.5μL dry dispensing using the Caliper Life Sciences Sciclone Advanced Liquid Handler (ALH) Workstation, configured with a Low Volume Head and 384/1536 Fixed-Cannula Array.

Materials and Methods

Instrumentation

All testing was performed using a Sciclone ALH with a Low Volume Dispensing Head, and a Bulk Reagent Dispenser (Caliper Life Sciences). Absorbance was measured at 450nm using a Wallac Victor2 1420 Multilabel Counter (PerkinElmer).

Reagents

Tartrazine was obtained from Sigma Chemical Company, Catalogue number T-0388. A stock dispensing solution was prepared by dissolving 2.8g of tartrazine into 1L of DMSO. The stock solution was prepared for dispensing volumes of 0.5μL. Deionized water (DI) was used as a diluent after dispensing into the dry plate.

Accuracy Determination

Dispense accuracy is determined before measuring dry dispense precision. A calibration is performed to determine the volume dispensed. For the calibration, 50μL of deionized water is pipetted in to a 384 Well Flat Bottom, Untreated Polystyrene Assay Plates (COSTAR, Catalogue # 3702). Next, 0.5μL of the tartrazine solution is manually injected into each of 20 wells, successively, from a filled 10μL Hamilton micro syringe. The wells are manually mixed by multiple aspirate- dispense cycles using a 50μL hand pip
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting FlashPlates on the Wallac MicroBeta
3. Direct Counting of Millipore MultiScreen Filtration Plates.
4. Sciclone ALH Bulk Dispense Precision Testing
5. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
6. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
7. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
8. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
9. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
10. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Dispensing Dry Plates with the Sciclone ALH

(Date:7/31/2014)... , July 31, 2014 The SNIS ... with $25,000 to fund a translational research project.  ... 2011, the gift was presented at the Society ... Meeting in Colorado Springs, CO. ... neurointerventional approaches to neurovascular conditions, SNIS formed the ...
(Date:7/31/2014)... Graphite Metallizing Corporation announces GRAPHALLOY® bearings ... are uniquely suited to prevent loss of lubrication that ... high temperatures . , Recently, a manufacturer of ... at Graphite Metallizing to overcome problems with movement of ... pans containing blocks of powder mixture are loaded onto ...
(Date:7/31/2014)... -- Research and Markets has announced the ... report to their offering. ... the genetic material or genomes of an organism. It ... discovery and development of diagnostic and therapeutic solutions for ... is supported by three major product segments: Instruments, Consumables, ...
(Date:7/31/2014)...  Smith & Nephew (LSE:SN, NYSE: ... announced its entry into the forefoot market with ... System. Comprised of three separate repair options, the ... repair and reconstruction, a metatarsal osteotomy guide, and ... fusion, also known as hammer toe correction. ...
Breaking Biology Technology:SNIS Foundation Bestows First Grant Award For Cutting-Edge NeuroInterventional Research 2SNIS Foundation Bestows First Grant Award For Cutting-Edge NeuroInterventional Research 3Graphite Metallizing Announces Bearings for High Temperature Oven Applications 2Graphite Metallizing Announces Bearings for High Temperature Oven Applications 3Global Genomics Market 2014-2018: Key Vendors are F. Hoffmann-La Roche, Life Technologies, Qiagen and Illumina 2Smith & Nephew scores a HAT-TRICK with its entry into the high-growth hammer toe repair market 2Smith & Nephew scores a HAT-TRICK with its entry into the high-growth hammer toe repair market 3Smith & Nephew scores a HAT-TRICK with its entry into the high-growth hammer toe repair market 4
... Advanstar|CBI recently announced the full agenda for their ... takes place March 6-7, 2012 in Baltimore, MD. ... Lilly, Genentech, AstraZeneca, Shire Human Genetic Therapies and many ... Meds recently interviewed PAP,s Senior Program Manager, Sharon Langan. ...
... Jan. 9, 2012   Veracyte, Inc ., a ... molecular cytology, today announced that Palmetto GBA, a ... coverage for the company,s Afirma Gene Expression Classifier ... that Veracyte,s genomic test is now accessible to ...
... Chembio Diagnostics, Inc. (OTC.PK: CEMI and OTC.QB: ... diagnostic tests, today announced that Lawrence A. Siebert, President ... Showcase in San Francisco at approximately 5:30 pm Eastern ... 2012.   The conference will be simultaneously ...
Cached Biology Technology:Veracyte Announces Medicare Coverage for Its Afirma® Gene Expression Classifier for Improved Thyroid Nodule Diagnosis 2Veracyte Announces Medicare Coverage for Its Afirma® Gene Expression Classifier for Improved Thyroid Nodule Diagnosis 3Chembio Presenting at Biotech Conference 2Chembio Presenting at Biotech Conference 3
(Date:7/31/2014)... German . , ... a sponge that soaks up liquids. Hence, these highly ... gases. However, loading of many MOFs is inhibited by ... report in Nature Communications that the barriers ... can be prevented by water-free synthesis and storing strategies. ...
(Date:7/31/2014)... 2014 -- Do people make a rational choice to ... that way? Is it a matter of genetics?, Two ... colleague from Rice University say that neither conscious decision-making ... left while others lean right. , A growing body ... are at the core of political differences, the researchers ...
(Date:7/31/2014)... the photovoltaic effect in 1839, humankind has sought to ... its own purposes. In a new research report published ... FASEB Journal , scientists may have uncovered a ... focusing on a naturally occurring combination of lipids that ... over time and across speciessuggests that this specific natural ...
Breaking Biology News(10 mins):Free pores for molecule transport 2Scientists find growing consensus: Political attitudes derive from body and mind 2Scientists shine bright new light on how living things capture energy from the sun 2
... as fit as the people of ancient Athens, despite all ... by University of Leeds (UK) exercise physiologist, Dr Harry Rossiter. ... rowing a reconstruction of an Athenian trireme, a 37m long ... portable metabolic analysers, he measured the energy consumption of a ...
... tiny piece of genetic code apparently goes where no bit ... its own internal code. , A report on the renegade ... be published Jan. 5 in Science. , MicroRNAs, already ... gum up larger strands of RNA that carry instructions for ...
... predict the color of plants on planets in other solar ... live on extra-solar planets, according to scientists whose two scientific ... The scientists studied light absorbed and reflected by organisms on ... the light given off by planets circling distant stars, they ...
Cached Biology News:Fitness has fallen since the days of Ancient Greece 2Renegade RNA -- Clues to cancer and normal growth 2NASA predicts nongreen plants on other planets 2NASA predicts nongreen plants on other planets 3
Cultrex 3D Culture Matrix Laminin I....
mitochondrial proteins (human heart) for 2-D gel electrophoresis *5 mg/mL*...
...
... GeneMaths XT is undoubtedly the most ... Its advanced concept of layers and subsets ... different layers for different data outputs, for ... values, etc., as well as different subsets ...
Biology Products: