( ...)DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid ,,, DNA purification

QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using a single plate and a simple bindwashelute procedure. The protocol is faster and easier to perform than existing two-plate methods. The DirectPrep 96 procedure is designed for use on BioRobot 3000 or BioRobot 8000 workstations, where 4 plates can be processed in parallel, and can be performed manually using the QIAvac Multiwell. The DirectPrep 96 Miniprep System yields plasmid DNA suitable for all routine applications, including automated fluorescent sequencing, restriction digestion, and PCR.

The DirectPrep 96 Miniprep System provides:

Economy for high-throughput sequencing and screening projects
Easy handling simple one-plate procedure
Flexibility automated or manual procedures available
Speed 96 samples in just 35 minutes (manual procedure)
SBS (Society for Biomolecular Screening) plate standard easy to automate

Flexible procedure DirectPrep 96 Procedure The DirectPrep 96 Miniprep method is a simple one-plate procedure. All lysis steps are carried out in the same 96-well flat-bottom block used for growth of the bacteria. After alkaline lysis of the pelleted cells, the lysate is neutralized using an optimized buffer, which leads to a virtually clear lysate. Timeconsuming centrifugation or filtration of the lysate is not required, a
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