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Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS

g>Conclusion

MALDI-TOF MS is a rapid and easy way of monitoring peptide synthesis. Small amounts of resin can quickly be treated with TFA and the peptides analyzed with no further treatment or purification. Mass measurement can be carried out on crude samples and be used to verify identity before final purification. Removal of protecting groups can be followed as a function of time and incomplete coupling can be identified very quickly.

Acknowledgements

PerSeptive Biosystems would like to acknowledge Arnold Falick and Keith Waddell of PE Biosystems for their contributions to this application note.


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