Navigation Links
Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS

tochondrial aspartate aminotransferase, ALLQSRLLLSAPRRAAATARA. A deprotection time of 1hour was used for these experiments. Had there been an issue with the synthesis, the exact problem step would have been diagnosed.

Figure 2 shows some of the minor peaks in the spectrum taken after step 13. Small amounts of deletion peptides are seen in addition to small peaks representing residual Pbf (Arg-protecting group). These are due to released Pbf attacking, for example, the Serine residue. Small contributions from cationization with sodium and potassium are also observed.

Figure 3 shows the MH+ region of the completed and deprotected peptide. Neurotensin (MW 1,673) and ACTH 18-39 (MW 2,465) were used as internal calibrants. The average error of the mass measurement was +/- 0.03 Da (5 replicates). This measurement is more than would be required to differentiate between a carboxyl and an amide (one Da difference). All masses are monoisotopic since the resolution of the instrument was sufficient to resolve isotopes at these masses.

1 hour deprotection is not required to determine if the synthesis is proceeding correctly. Figure 4 shows the peptide data after step 10 with a 10 minute deprotection step. The Pbf protecting group on Arg is clearly still present but some fully deprotected product (Fmoc peptide) is visible. The t-Bu protecting group on Thr is completely absent. No t-Bu group was detected after > 5 minute treatment with TFA. The trityl group used to protect Gln behaves similarly.

The result in Figure 5 is from the deprotection of the synthesis of the peptide SEHFLANES-OH. In this case the deprotection step was for 5 minutes only, and the presence of trityl and up to 3 t-butyl groups can be detected.

The full time course experiment up to 1 hour is seen in Figure 6. After 1 hour the unprotected peptide is the principal peak in the spectrum.

'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Constructing Directional cDNA Libraries from Limited Amounts of RNA
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
6. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
7. TripleMaster PCR System: Direct Comparison with Competitors
8. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
9. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
10. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
11. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Direct Monitoring Solid Phase Peptide Synthesis MALDI TOF

(Date:5/5/2015)... 2015 Psyche Systems Corporation and Luminate Health ... first-of-its-kind platform that helps labs comply with new federal ... the way patients engage with these results, and improves ... provides a HIPAA-compliant digital platform that gives labs an ... their lab results, and uniquely connects labs, physicians, and ...
(Date:5/5/2015)... , May 5, 2015 ImmunoCellular ... Andrew Gengos , President and Chief Executive ... 7, 2015. DATE:  Thursday, May 7, 2015     ... http://VirtualInvestorConferences.com > click the red "register/ ... a live, interactive online event where investors are ...
(Date:5/5/2015)... 2015 Research and Markets ( ... "Clinical Laboratory, Molecular Diagnostics and Genomic Testing ... - Europe Version" report to their offering. ... area of molecular diagnostics and pharmacogenics hold the ... market that is moving out of the national ...
(Date:5/5/2015)... College Station, TX (PRWEB) May 05, 2015 ... Pfizer were announced last week as recipients of ... Technology Innovation in recognition of their Portable, Continuous, ... system that integrates a small footprint, continuous processing ... GMP modules) to process API powders and inactive ...
Breaking Biology Technology:Psyche Systems Corporation Forms Strategic Alliance with Luminate Health to Provide First-of-its-kind Patient Access Solution 2Psyche Systems Corporation Forms Strategic Alliance with Luminate Health to Provide First-of-its-kind Patient Access Solution 3ImmunoCellular Therapeutics to Webcast Live at VirtualInvestorConferences.com on May 7 2European Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services Market 2015-2019 2GEA Pharma Systems, G-CON Manufacturing and Pfizer Consortium Receives Best Technology Innovation Award at INTERPHEX 2015 2GEA Pharma Systems, G-CON Manufacturing and Pfizer Consortium Receives Best Technology Innovation Award at INTERPHEX 2015 3
... May 11, 2011 SeraCare Life Sciences, Inc. (NASDAQ: ... providing vital products and services to facilitate the discovery, ... announced the election of Joseph Nemmers and Kevin Hrusovsky ... Mr. Nemmers has been appointed to the position of ...
... NEW YORK, May 11, 2011 ... new market research report is ... Biotech M&A and ... are Poised to Increase in ...
... 7, 2011) Research being presented at Digestive ... significantly increased benefits for qualifying patients undergoing bariatric ... to be considered when determining whether the surgery ... international gathering of physicians and researchers in the ...
Cached Biology Technology:SeraCare Announces the Election of Joseph Nemmers and Kevin Hrusovsky to the Board of Directors 2SeraCare Announces the Election of Joseph Nemmers and Kevin Hrusovsky to the Board of Directors 3SeraCare Announces the Election of Joseph Nemmers and Kevin Hrusovsky to the Board of Directors 4Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 2Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 3Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 4Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 5Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 6Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 7Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 8Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 9Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 10Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 11Reportlinker Adds Biotech M&A and Licensing Strategies - Co-development Agreements are Poised to Increase in the Future to Counter Drying Pipelines 12New approaches, technological advances offer promise for obesity treatment 2
(Date:3/24/2015)... 2015   NexID Biometrics LLC, whose spoof-mitigation ... today announced the beginning of shipments of version 2.0 ... The company, based in Potsdam, N.Y. ... Expo, which began here today at the Walter E. ... of its SDK boosts the accuracy rate range to ...
(Date:3/23/2015)... March 23, 2015  In the 2014 fiscal year, ... conviction losses. This figure accounts for a fraction of ... The Nilson Report, exceeds $11 billion globally. The polygraph ... a crime has occurred. Investor Mark Saint Juste ... Joe Paolella partnered to administer the test ...
(Date:3/20/2015)... , March 20, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market, ... is one of the products featured in 2015 "I ... at 11:00 PM EST on the DIY Network.   ... Las Vegas , site of the 2015 ...
Breaking Biology News(10 mins):NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2Polygraph Test Now Used to Protect Investors From Fraud 2Polygraph Test Now Used to Protect Investors From Fraud 3NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 2NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 3
... Researchers in the Department of Physics and Astronomy ... synthesis method, which has led them to the discovery ... the uptake of drugs by the body,s cells. ... of the new method is the independent control of ...
... at the Sahlgrenska Academy, University of Gothenburg, Sweden, have ... involving the stomach hormone ghrelin. When ghrelin,s actions in ... system are reduced. It is an important discovery that ... alcohol dependence. The results will be published in ...
... to produce high-resolution fluorescence images from this depth because of ... journal, the Munich researchers describe how they can reveal ... to light". In the future this technology may facilitate the ... Since the dawn of the microscope scientists have been using ...
Cached Biology News:University of Leicester researchers discover new fluorescent silicon nanoparticles 2Discovery may provide new treatments for alcohol dependence 2The sound of light: Innovative technology shatters the barriers of modern light microscopy 2
Sheep polyclonal to Malate Dehydrogenase ( Abpromise for all tested applications). Antigen: Full length protein - Pig heart malate dehydrogenase. Entrez Gene ID: 4190 Swiss Protein ID: P...
HAMP Immunogen: HAMP (AAH20612, 25 a.a. ~ 85 a.a) full length recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
... Antigen peptide transporter 2 ... (Peptide transporter PSF2) (Peptide ... (Peptide transporter involved in ... Antigen: ...
Raised to arginine vasopressin coupled to bovine thyroglobulin with carbodiimide ....
Biology Products: