Navigation Links
Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS

Overview

Mass spectrometry has proven to be a valuable tool in verifying and improving peptide synthesis. Matrix Assisted Laser Desorption Ionization (MALDI) Time-of-Flight (TOF) MS is particularly useful in that it is rapid, easy to use and requires relatively little maintenance. This work shows the ability of MALDI-TOF MS to follow the progress of solid phase peptide synthesis. This work was first presented by A. Falick, G. Tarr and N. Sole at ABRF 96.

Key Features

Identification of peptide synthesis products

Ability to track reactions over time

Mass accuracy in DE linear mode

Method

PEPTIDE SYNTHESIS

Linear syntheses were carried out using Fmoc chemistry in the C to N direction on a PerSeptive Biosystems 9050 Plus continuous flow synthesizer. Coupling times were 1 hour, using 4 equivalents of amino acid/ activator and 8 of base. HATU, DIEA and DMF were used as solvents.

MASS SPECTROMETRY

Samples were analyzed on a Voyager-DE STR Workstation in linear mode. The laser was set at 337 nm and 50-100 laser shots were accumulated per spectrum.

Peptides were deprotected with 95% TFA at room temperature for the times noted. 1 mg of resin was treated with 20 μl of TFA, then a 1μl aliquot was removed and added to 50 μl of water. 1 μl aliquot of this diluted sample was mixed with 4 μl of a 10 mg/ml solution of alpha cyano 4-hydroxy cinnamic acid in 60% ethanol and 40% acetonitrile. A 0.5 μl droplet was placed on the MALDI target, air-dried and the target inserted into the instrument.

Results

Figure 1 shows the progress of the synthesis of the 21 mer peptide fragment from the targetting sequence of the precursor of mi
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Constructing Directional cDNA Libraries from Limited Amounts of RNA
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
6. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
7. TripleMaster PCR System: Direct Comparison with Competitors
8. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
9. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
10. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
11. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Direct Monitoring Solid Phase Peptide Synthesis MALDI TOF

(Date:10/19/2014)... October 19, 2014 The Asia-Pacific ... market in Asia-Pacific with analysis and forecast of ... Asia Pacific Bromine Market report, to get an ... provides a glimpse of the segmentation in the ... tables and figures. , http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 The Asia-Pacific ... Analytics market in Asia-Pacific with analysis and forecast of ... million in 2014 to $208 million by 2019, at ... 2014 to 2019. , Browse through the TOC of ... idea of the in-depth analysis provided. It also provides ...
(Date:10/19/2014)... Orthopedic braces and support systems report defines and segments the ... The Orthopedic braces and support systems market in Asia is ... a developing CAGR of 4.4% from 2013 to 2018. , ... support systems market, to get an idea of the in-depth ... of orthopedic braces and support systems market in the same ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 According ... Products (Access Points; WLAN Controllers; Wireless gateways)]- Global ... and segments the global Outdoor Wi-Fi market with ... this report MarketsandMarkets also identifies the drivers and ... on the trends, opportunities and challenges. , Browse ...
Breaking Biology Technology:The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 2The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 4Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 2Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 4
... of Farletuzumab for Ovarian CancerAgreement Highlights Lonza,s Ability ... and BASEL, Switzerland, May 19 Morphotek(R), Inc., ... and Lonza Group Ltd jointly announced today that ... support the development and manufacturing of a subset ...
... -- Leading pharmaceutical and biotech,companies are drawing on ... enhance their R&D productivity, leverage academic,insights and diversify ... a population,base that is representative of key Asian ... companies to test and develop new solutions for,Asia ...
... have signed an agreement-in-principle to ensure the valorization and the ... at the Faculty of veterinary medicine in St-Hyacinthe, Quebec, Canada, ... ... Laval, Quebec (PRWEB) May 18, 2009 -- Universite ...
Cached Biology Technology:Morphotek(R), Inc and Lonza Group Ltd Enter Into Manufacturing Services Agreement 2Morphotek(R), Inc and Lonza Group Ltd Enter Into Manufacturing Services Agreement 3Morphotek(R), Inc and Lonza Group Ltd Enter Into Manufacturing Services Agreement 4Pharma and Biotech Companies Plug into Singapore's Integrated Research Network 2Pharma and Biotech Companies Plug into Singapore's Integrated Research Network 3Pharma and Biotech Companies Plug into Singapore's Integrated Research Network 4Pharma and Biotech Companies Plug into Singapore's Integrated Research Network 5
(Date:10/18/2014)... a sample of patients with undiagnosed, suspected genetic conditions, ... with a higher molecular diagnostic yield than traditional molecular ... JAMA . The study is being released to coincide ... , Exome sequencing, which sequences the protein­coding region of ... material present in a cell or organism), has been ...
(Date:10/16/2014)... the human body. Battles are won, lost or ... this stalemate—known as tumor dormancy—can last up to ... that is poorly understood. , A new ... Torquato, a Professor of Chemistry at Princeton University, ... and the switch to a malignant state. Published ...
(Date:10/16/2014)... Syncytial Virus causes severe respiratory tract infections and ... year. Scientists at VIB and Ghent University have ... counteract this common virus infection. , Xavier ... that paves the way for the development of ... virus that causes suffering in numerous small children ...
Breaking Biology News(10 mins):Study examines type of exome sequencing and molecular diagnostic yield 2Study examines type of exome sequencing and molecular diagnostic yield 3Modeling tumor dormancy 2New perspectives for development of an RSV vaccine 2
... HOUSTON -- (June 10, 2010) Most of the ... with just a few producing blood cells and maintaining ... bacteria can be a call-to-arms, awaking the sleeping stem ... that fight the foreign organisms. The "bugler" that awakes ...
... From April 8 to 10, 2010, 185 attendees ... on advancing new tools to humanely and effectively manage ... in reproductive biology and other related scientific disciplines presented ... in animal health drug development. Veterinary leaders joined with ...
... men have surgery to remove a cancerous prostate gland, up ... have an erection because of damage to a critical nerve ... Northwestern University Feinberg School of Medicine shows the damaged nerve ... hedgehog delivered via a nanofiber gel. The study, ...
Cached Biology News:Gamma interferon a wake-up call for stem cell response to infection 2Gamma interferon a wake-up call for stem cell response to infection 3Momentum builds after 4th International Symposium on Pet Contraception 2Momentum builds after 4th International Symposium on Pet Contraception 3Protein could heal erectile dysfunction after cancer surgery 2Protein could heal erectile dysfunction after cancer surgery 3
Mouse MMP-24 (MT5-MMP) MAb (Clone 143908)...
TMB Slow Kinetic One Component HRP Microwell Substrate, 1 L...
Request Info...
... Intestinal (CIAP), catalyzes the hydrolysis of 5-phosphate ... deoxyribonucleoside triphosphates. This enzyme is used to ... vector DNA by removing phosphate groups from ... for the dephosphorylation of 5 phosphorylated ends ...
Biology Products: