Navigation Links
Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS

Overview

Mass spectrometry has proven to be a valuable tool in verifying and improving peptide synthesis. Matrix Assisted Laser Desorption Ionization (MALDI) Time-of-Flight (TOF) MS is particularly useful in that it is rapid, easy to use and requires relatively little maintenance. This work shows the ability of MALDI-TOF MS to follow the progress of solid phase peptide synthesis. This work was first presented by A. Falick, G. Tarr and N. Sole at ABRF 96.

Key Features

Identification of peptide synthesis products

Ability to track reactions over time

Mass accuracy in DE linear mode

Method

PEPTIDE SYNTHESIS

Linear syntheses were carried out using Fmoc chemistry in the C to N direction on a PerSeptive Biosystems 9050 Plus continuous flow synthesizer. Coupling times were 1 hour, using 4 equivalents of amino acid/ activator and 8 of base. HATU, DIEA and DMF were used as solvents.

MASS SPECTROMETRY

Samples were analyzed on a Voyager-DE STR Workstation in linear mode. The laser was set at 337 nm and 50-100 laser shots were accumulated per spectrum.

Peptides were deprotected with 95% TFA at room temperature for the times noted. 1 mg of resin was treated with 20 μl of TFA, then a 1μl aliquot was removed and added to 50 μl of water. 1 μl aliquot of this diluted sample was mixed with 4 μl of a 10 mg/ml solution of alpha cyano 4-hydroxy cinnamic acid in 60% ethanol and 40% acetonitrile. A 0.5 μl droplet was placed on the MALDI target, air-dried and the target inserted into the instrument.

Results

Figure 1 shows the progress of the synthesis of the 21 mer peptide fragment from the targetting sequence of the precursor of mi
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Constructing Directional cDNA Libraries from Limited Amounts of RNA
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
6. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
7. TripleMaster PCR System: Direct Comparison with Competitors
8. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
9. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
10. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
11. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Direct Monitoring Solid Phase Peptide Synthesis MALDI TOF

(Date:8/20/2014)... Clintrax Global, Inc., a worldwide provider ... Raleigh, NC , recently announced the addition ... of Administration. As Vice President ... including their alignment with company objectives and policies.  ... an HR executive, specializing in needs assessments, customer ...
(Date:8/20/2014)... analysis of patented university inventions licensed to biotechnology ... to commercialization. To open these roadblocks, the researchers ... during the discovery stage could lead to faster ... frequently derived from discoveries made in university laboratories ... known during clinical trials, which have a high ...
(Date:8/20/2014)... have it that American Indians largely were wiped out ... and tuberculosis brought to the New World by European ... million people lived in the Americas shortly before Europeans ... by European diseases., But new research led by anthropological ... Krause of the University of Tubingen in Germany indicates ...
(Date:8/20/2014)... chemists have succeeded in measuring vibrational motion of a ... reveals how vibration of a single molecule differs from ... study was performed at the University of California, Irvine, ... Jyvskyl works as a visiting fellow under professor Vartkess ... second team was lead by Professor Eric O. Potma. ...
Breaking Biology Technology:Clintrax Global, Inc. Announces Addition to Executive Team 2Early bottlenecks in developing biopharmaceutical products delay commercialization 2Early bottlenecks in developing biopharmaceutical products delay commercialization 3Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3Seeing a molecule breathe 2
... provides patients with prescription drug,information, encourages patients ... iMedica Corporation, a leading,developer of healthcare ... it has partnered with LDM GROUP, a ... practices using,the iMedica Patient Relationship Manager(TM) (PRM), ...
... BG ), an integrated, global agribusiness and food ... 30, 2008, on Thursday,October 23, 2008, prior to the ... conference call at 10:00 a.m.,EDT on October 23, 2008, ... the conference call, please dial (877) 719-9791. If you,are ...
... A new company is,being created as a ... interventions against chronic inflammatory and infectious,diseases. BioTherapeutics ... discovery and development of nutraceuticals -- naturally ... products and functional,food ingredients., BioTherapeutics Inc. ...
Cached Biology Technology:iMedica Partners With LDM GROUP to Offer ScriptGuide to Physician Practice Customers 2iMedica Partners With LDM GROUP to Offer ScriptGuide to Physician Practice Customers 3Bunge Limited Schedules Third Quarter 2008 Earnings Release and Conference Call 2New Company to Develop Nutritional Interventions Against Chronic Inflammatory and Infectious Diseases 2New Company to Develop Nutritional Interventions Against Chronic Inflammatory and Infectious Diseases 3
(Date:8/20/2014)... HOUSTON, Aug. 20, 2014 George E. Fox, a John ... University of Houston (UH), was named a fellow in the ... (ISSOL). , Fox is one of four members ... Spain chosen as fellows in 2014. Fellows are elected ... With more than 500 members from more than 20 countries, ...
(Date:8/20/2014)... In a study of 158 pregnant teenagers in Rochester, ... and intentional consumption of ice, cornstarch, vacuum dust, baby ... new Cornell study. , Moreover, such teens had significantly ... not eat nonfood substances. , Pregnant teens, regardless of ... can lead to iron deficiency and anemia. Low iron ...
(Date:8/20/2014)... Institute (HCI) at the University of Utah have identified ... BCR-ABL, the unregulated enzyme driving the blood cancer chronic ... nearly 6,000 new cases of CML will be diagnosed ... kinase inhibitors (TKIs), target BCR-ABL and are effective at ... control it in a way that allows patients to ...
Breaking Biology News(10 mins):UH professor named fellow by International Astrobiology Society 2Pica in pregnant teens linked to low iron 2Blueprint for next generation of chronic myeloid leukemia treatment 2
... 2011 The Center for Engineering in Medicine (CEM) ... Hospital (MGH), a Harvard Teaching Hospital -- has announced ... in conjunction with event organizer Horizon House Ltd.  The ... School on July 14-15, and expects to draw over ...
... April 19, 2011 Vobile, a leading provider ... Youku.com Inc., China,s leading Internet television company, announced today ... protection against copyright infringement through upgrades to its ... its own copyright identification management platform, the first ...
... Looking to tap into the resources of academia, ... announced May 2 that it is giving prominence to ... (NNR). This designation will address specific science and ... as the corrosive impact of the saltwater environment on ...
Cached Biology News:Massachusetts General Hospital Center Reignites Methods in Bioengineering Conference 2Youku Upgrades Fingerprinting System for Tougher Copyright Protection Measures 2Youku Upgrades Fingerprinting System for Tougher Copyright Protection Measures 3ONR puts spotlight on importance of sea-based aviation science and technology 2
Topoisomerase I...
...
... α-Secretase Activity Kit ADAM17 Activity Kit ... 96 Tests. PROTECT FROM LIGHT. AVOID FREEZE/THAW ... a specific and sensitive assay designed to ... and biological samples and for screening enzyme ...
... agarose-digesting enzyme developed by Promega for simple ... RNA from agarose gels. The enzyme is ... agarose melted at 6575C does not have ... before hydrolysis. Simply place the tube containing ...
Biology Products: