Navigation Links
Direct Counting of Millipore MultiScreen Filtration Plates.

ove cell growth. 3T3 cells (ATCC, Rockville, Maryland) were cultured to 104 cells per well and stimulated with Bovine Pituitary Extract (Collaborative Biomedical Products, #40123) at levels ranging from 0 to 250 ug/mL for 18 hours. The cells were then pulsed with 3H-thymidine for three to four hours prior to washing on the MultiScreen vacuum manifold. Two plates were prepared. The first was processed by punching the individual membrane disks into LS vials, solubilizing with 0.42% bleach, and mixing with 5 mL LS cocktail (Optifluor, Packard Instrument Company). The second plate was processed by adding 20 mL MicroScint-O to each well using a semi-automatic multichannel pipet and counting on TopCount.

Stimulation curves obtained with the TopCount and with conventional LSC are shown in Figure 4. Both methods yield the same results.

Other Assays.
A variety of other assays such as enzyme inhibition and kinase assays have been performed with the MultiScreen filtration plate on TopCount. Counting efficiencies for the radionuclides used in these assays are summarized in Table 2. For weak beta emitters such as 3H, 35S, and 14C, crosstalk was negligible. For gamma emitters (125I) and higher energy beta emitters (32P), crosstalk was less than 0.5%.

Conclusions

The Millipore MultiScreen Assay system is designed for a variety of microplate assays requiring in plate incubations and filtration. A significant limitation of the system is the need to individually process the 96 samples in a plate for quantitation. The TopCount Microplate Scintillation Counter can facilitate the processing of MultiScreen filtration plates by allowing the user to process all 96 samples as a single unit. Crosstalk due to physical migration of radiolabel is not possible due to the discrete filter disks i
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Constructing Directional cDNA Libraries from Limited Amounts of RNA
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
6. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
7. TripleMaster PCR System: Direct Comparison with Competitors
8. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
9. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
10. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
11. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Direct Counting Millipore MultiScreen Filtration Plates

(Date:7/31/2014)... Food and natural product manufacturers, ... food alternatives that offer variety, as well as, ... market has numerous players operating in the marketplace ... Technology (VFT). VFT is a science-driven functional ... all tested to produce stable, effective and repeatable ...
(Date:7/31/2014)... (1912-1954), is famous for a number of breakthroughs, which ... he published a paper, which laid the foundation of ... computer algorithm. He next played a pivotal role in ... the German military codes, enabling the Allies to defeat ... late 1940,s he turned his attention to artificial intelligence ...
(Date:7/31/2014)... New York , July 31, 2014 ... by Transparency Market Research "Quantum Dots Market - Global ... - 2023," the market was valued at USD 88.5 ... 8,246.8 million by 2023, growing at a CAGR of ... full Quantum Dots Market Report at  http://www.transparencymarketresearch.com/quantum-dots.html ...
(Date:7/31/2014)... July 31, 2014 Research and Markets ... Genomics Market 2014-2018" report to their offering. ... is the study of the genetic material or genomes ... strong basis for the discovery and development of diagnostic ... or health conditions. Genomics is supported by three major ...
Breaking Biology Technology:Functional Ingredient Manufacturer – Vital Force Technology Infuses Raw Materials & Ingredients with Subtle Energy Formula 2A mathematical theory proposed by Alan Turing in 1952 can explain the formation of fingers 2A mathematical theory proposed by Alan Turing in 1952 can explain the formation of fingers 3Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 2Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 3Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 4Global Genomics Market 2014-2018: Key Vendors are F. Hoffmann-La Roche, Life Technologies, Qiagen and Illumina 2
... Boston College has been awarded a $1 million grant ... of university researchers developing a new microscope that uses ... and macroscopic matter with significantly improved clarity. The ... new class of microscopes known as "superlenses," which function ...
... 3, 2011 Ron Van Beek, president and CEO ... Synacore Digestive Support for dogs and cats, available exclusively ... synbiotic digestive support supplement containing both probiotics and prebiotics ... microflora in a dog,s or cat,s GI tract.  Van ...
... 3, 2011 Genomic Health, Inc. (Nasdaq: ... successfully surveyed expression of the whole human transcriptome ... Inc., to test hypotheses for biomarker discovery in ... study identified hundreds of coding and non-coding transcripts ...
Cached Biology Technology:Boston College receives W.M. Keck Foundation funding for nanoscale optical microscope 2Van Beek Natural Science Announces New Synacore Digestive Support Product 2Genomic Health Announces Results from Biomarker Discovery Program Utilizing Next-Generation Sequencing to Compare Normal and Tumor Breast Tissue 2Genomic Health Announces Results from Biomarker Discovery Program Utilizing Next-Generation Sequencing to Compare Normal and Tumor Breast Tissue 3Genomic Health Announces Results from Biomarker Discovery Program Utilizing Next-Generation Sequencing to Compare Normal and Tumor Breast Tissue 4
(Date:7/31/2014)... who studied 100 twin pairs have identified a gene mutation ... than six hours of sleep per night. The genetic ... of sleep deprivation. , Results show that a participant with ... an average nightly sleep duration of only five hours, which ... who slept for about six hours and five minutes per ...
(Date:7/31/2014)... team of scientists from Spain, France, and the ... that is the oldest definitive member of the ... amber. The discovery and description were made possible ... synchrotron imaging technique, which allows the detailed study ... new species is described in the journal ...
(Date:7/31/2014)... alters how the brain processes speech, potentially increasing ... neuroscientists at The University of Texas at Dallas. ... Ear and Hearing , researchers demonstrated for ... the brain,s recognition of speech sounds. , Noise-induced ... population, affecting an estimated 15 percent of Americans ...
Breaking Biology News(10 mins):Study of twins discovers gene mutation linked to short sleep duration 2UT Dallas study reveals effect of loud noises on brain 2
... Our brains, it turns out, are eco-friendly. A study published ... Venkatesh Murthy and Jakob Sorensen reveals that our brains have ... cells generate and propagate nerve impulses, or action potentials, by ... and out of the cells. Re-establishing the ion equilibrium after ...
... MADISON, WI, OCTOBER 12, 2009 Areas of ... and recreational (not grazing) purposes, i.e. "urban grasslands" are ... are over 150,000 km2of urban grasslands in the U.S. ... about nutrient runoff to streams, lakes, and estuaries and ...
... 13 CareFusion Corporation (NYSE: CFN ), a new ... the EnVe(TM) ventilator - a high-performance critical care ventilator for ... ventilator is 70-80 pounds lighter than other intensive care ventilators ... help hospitals increase the quality of patient care and decrease ...
Cached Biology News:Nitrogen mysteries in urban grasslands 2Nitrogen mysteries in urban grasslands 3CareFusion Launches EnVe(TM) Ventilator: 10-Pound High-Performance Critical Care Ventilator 2CareFusion Launches EnVe(TM) Ventilator: 10-Pound High-Performance Critical Care Ventilator 3
UltraMount is formulated for coverslipping sections stained with alcohol soluble chromogens, such as Fast-Red or AEC. No heating is required prior to use. Coverslips may be removed by soaking in wate...
Agarose, Biotechnology grade, 500g...
AM100 is an aqueous-based, permanent mounting media designed for use with alkaline phosphatase and immunoperoxidase stained tissue sections....
... c-Jun proto-oncogene was first identified as the cellular ... Jun B and Jun D have been shown ... C-terminal regions, which are involved in dimerization and ... involved in transcriptional activation, diverge. ...
Biology Products: