In the present study, we compared the gene expression profiles of the luminal epithelial endometrium of implantation (IM) and interimplantation (INTER) sites to elucidate the genes involved in the process of embryo apposition prior to implantation using LCM, linear amplification of RNA and microarray analysis. The nature of these genes suggest and that active tissue remodeling is in progress at the implantation sites before the embryonic attachment. Further, we propose that Ca2+ is a crucial regulatory factor, actively involved in this process. The list of genes identified may provide information regarding molecular mechanisms occurring at the implantation sites before embryo attachment and regulated by various embryonic factors.
1 Reese, J., Das, S.K., Paria, B.C., Lim, H., Song, H., Matsumoto, H., Knudtson, K.L., DuBois, R.N., Dey, S.K. (2001) Global gene expression analysis to identify molecular markers of uterine receptivity and embryo implantation. J Biol Chem, 276, 4413744145.
2 Carson, D.D., Lagow, E., Thathiah, A., Al-Shami, R., Farach-Carson, M.C., Vernon, M., Yuan, L., Fritz, M.A., Lessey, during the early to mid-luteal (receptive phase) transition in human endometrium detected by high-density microarray screening. Mol Human Reprod, 8, 871-879.
3 Yoon, S.J., Choi, D.H., Lee, W.S., Cha, K.Y., Kim, S.N., Lee, K.A. (2004) A molecular basis for embryo apposition at the luminal epithelium. Mol Cell Endocrinol, 219, 95-104.
The data described herein has been published, in part, in Yoon et al.,