Navigation Links
Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

s). Because we used intensities from the IM sample in the numerator portion of gene expression ratio, the positively significant genes could be interpreted as up-regulated genes in IM samples.

The microarray data from quadruplet hybridizations for each sample were normalized with LOWESS and visualized by MA scatter plot (Fig. 3). The horizontal axis of Figure 3 (Mean of A) represents the signal intensity and the vertical axis (Mean of M) represents the gene expression difference. Representatives from significant genes determined by SAM were indicated with black arrow. Some non-significant genes with high M values were marked with red arrows.

Figure 4 depicts clustering visualization of our data. This figure is a pseudo-color representation of M values of 88 genes that were identified as significant in SAM analysis. The M value was color-coded according to its numeric value using Treeview software. The red color indicates up-regulated gene expression, while the green color indicates down-regulated gene expression.

Comparison by SAM revealed that 73 genes were more highly expressed at the IM site and 13 at the INTER site, showing higher expression within an estimated false discovery rate (FDR) of 0.163. These differentially expressed genes at each site were categorized based on the basis of the best available information regarding their biological functions. Among 73 highly expressed genes at the IM site, 6 were Expressed Sequence Tags (ESTs) and 14 were genes with unknown functions. The remaining 53 genes with known functions were categorized as being structural proteins (24, 45.3%) or as being related to metabolism (6, 11.3%), signal transduction (7, 13.2%), immune responsiveness action (6, 11.3%), cell cycling (4, 7.5%), gene/ protein expression (4, 7.5%), and oxidative stress (2, 3.8%). Meanwhile, among 13 genes that were up-regulated at the INTER site, 2 were ESTs and 6 were genes with unknown functions. The remaining set of 5 genes had functions relate
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Fractionating DNA Fragments Generated by Differential Display PCR
2. Identification of Differentially Expressed Gene Products with the CastAway System*
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
5. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
6. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
7. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
8. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
9. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. A New Lambda Vector for Mammalian Expression
Post Your Comments:
(Date:1/22/2015)... Dr. Greg Leyer of UAS Labs recently was ... Pre-Conference seminar on probiotics in San Diego, CA. , ... conference for health care professionals. This year’s pre-conference seminar was ... in health. Dr. Leyer spoke about the emerging topics and ...
(Date:1/22/2015)... NEW BRUNSWICK , Nueva Jersey , 22 ... Award for Biomedical Research abre hoy su llamada ... las personas cuya investigación científica ha hecho, o tiene ... de la salud humana. Las nominaciones se aceptarán hasta ...
(Date:1/22/2015)... Jan. 22, 2015  Derma Sciences, Inc. (Nasdaq: ... on advanced wound care, announces that AMNIOEXCEL® and ... been added to the Premier, Inc. Regenerative Skin ... for the AMNIOEXCEL® and AMNIOMATRIX® product lines, which ...
(Date:12/24/2014)... , Dec. 24, 2014  Vermillion, Inc. (Nasdaq:   ... disease, announced today that the Company closed its ... Investment Management, Jack W. Schuler , Birchview ... were $10.5 million, before offering expenses.  The proceeds ...
Breaking Biology Technology:El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3
... Genetics Policy Institute  (GPI) joined the Coalition for Advancement ... of the court") brief in the United States Court of ... for human embryonic stem cell research. GPI ... and in the Court of Appeals, the latter in collaboration ...
... Feb. 27, 2012 PRO-DEX, INC. (Nasdaq: ... horsepower motor business, operating under the name Pro-Dex Astromec ... the terms of the asset purchase agreement, executed on ... assets of Astromec, consisting of inventory, equipment and intangibles, ...
... MAPLE GROVE, Minn., Feb. 27, 2012  Nausea and vomiting in ... affecting an estimated 70 to 85% of all pregnant women.(1) ... nurse practitioners (NPs), only 40% of their patients reported experiencing ... for greater patient and healthcare provider dialogue about the condition. ...
Cached Biology Technology:Genetics Policy Institute (GPI) Files Additional Legal Arguments in Sherley v. Sebelius Stem Cell Case 2Pro-Dex, Inc. Announces the Sale Of Its Motor Business 2Morning Sickness or NVP: National Survey of Nurse Practitioners Points to Underreporting of Common Condition in Pregnancy 2Morning Sickness or NVP: National Survey of Nurse Practitioners Points to Underreporting of Common Condition in Pregnancy 3Morning Sickness or NVP: National Survey of Nurse Practitioners Points to Underreporting of Common Condition in Pregnancy 4Morning Sickness or NVP: National Survey of Nurse Practitioners Points to Underreporting of Common Condition in Pregnancy 5
(Date:1/22/2015)... /PRNewswire/ - Today, FindBiometrics, the leading source of biometrics industry news, ... website design. "When we launched FindBiometrics 12 years ... O,Neill , founder and CEO of FindBiometrics. "Now it,s maturing ... the key players on a very broad scale.  We are ...
(Date:1/22/2015)... Showcase, Hall E -   EyeLock Inc. , a market ... showcase its EyeLock ID technology integrated in a 3D printed ... Oak Ridge National Laboratory (ORNL) at the 2015 ... technology is being used to validate the driver and authorize ...
(Date:1/22/2015)... Jan. 16, 2015  A man-made form of insulin delivered ... mental capabilities in adults with mild cognitive impairment and ... by researchers at Wake Forest Baptist Medical Center. ... amnesic mild cognitive impairment (MCI) or mild to moderate ...
Breaking Biology News(10 mins):Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3
... key organ in the immune system in a development that ... that it is possible to convert one stem type to ... who used rat models, grew stem cells from the thymus ... the laboratory using conditions for growing hair follicle skin stem ...
... LAKE CITYFollowing up a pioneering 2007 proof-of-concept study, a ... promising new anti-HIV drug candidate, PIE12-trimer, that prevents HIV ... Ph.D., associate professor of biochemistry in the University of ... study published Wednesday, Aug. 18, 2010, online by the ...
... has revealed the oldest known evidence of a macabre part ... turn them into zombies. The discovery has been made ... from the University of Exeter, who studies parasites that can ... of animals are susceptible to the often deadly body invasion, ...
Cached Biology News:Stem cell versatility could help tissue regeneration 2Potential HIV drug keeps virus out of cells 2Fossil reveals 48-million-year history of zombie ants 2
...
... MagCellect Ferrofluid products perform optimally ... R&D Systems MagCellect Magnet. To ... neodymium iron boron permanent magnet ... 75 mm test tubes or ...
S. O'Hare & C.K. Atterwill • In vitro toxicity techniques for regular lab use. Ex vivo techniques are also covered with the aim of reducing and refining the use of animals in the future....
Agarase cleaves agarose to neoagaro-oligosaccharides, and is used for,quantitative, gentle recovery of DNA from low melting point agarose....
Biology Products: