Navigation Links
Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

s). Because we used intensities from the IM sample in the numerator portion of gene expression ratio, the positively significant genes could be interpreted as up-regulated genes in IM samples.

The microarray data from quadruplet hybridizations for each sample were normalized with LOWESS and visualized by MA scatter plot (Fig. 3). The horizontal axis of Figure 3 (Mean of A) represents the signal intensity and the vertical axis (Mean of M) represents the gene expression difference. Representatives from significant genes determined by SAM were indicated with black arrow. Some non-significant genes with high M values were marked with red arrows.

Figure 4 depicts clustering visualization of our data. This figure is a pseudo-color representation of M values of 88 genes that were identified as significant in SAM analysis. The M value was color-coded according to its numeric value using Treeview software. The red color indicates up-regulated gene expression, while the green color indicates down-regulated gene expression.

Comparison by SAM revealed that 73 genes were more highly expressed at the IM site and 13 at the INTER site, showing higher expression within an estimated false discovery rate (FDR) of 0.163. These differentially expressed genes at each site were categorized based on the basis of the best available information regarding their biological functions. Among 73 highly expressed genes at the IM site, 6 were Expressed Sequence Tags (ESTs) and 14 were genes with unknown functions. The remaining 53 genes with known functions were categorized as being structural proteins (24, 45.3%) or as being related to metabolism (6, 11.3%), signal transduction (7, 13.2%), immune responsiveness action (6, 11.3%), cell cycling (4, 7.5%), gene/ protein expression (4, 7.5%), and oxidative stress (2, 3.8%). Meanwhile, among 13 genes that were up-regulated at the INTER site, 2 were ESTs and 6 were genes with unknown functions. The remaining set of 5 genes had functions relate
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Fractionating DNA Fragments Generated by Differential Display PCR
2. Identification of Differentially Expressed Gene Products with the CastAway System*
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
5. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
6. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
7. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
8. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
9. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. A New Lambda Vector for Mammalian Expression
Post Your Comments:
(Date:9/19/2014)... , Sept. 19, 2014 Pfenex Inc. (NYSE ... development of high-value and difficult to manufacture proteins including ... at the 21 st Annual NewsMakers in the ... . Bertrand Liang , chief executive officer of ... programs and business strategy on Friday, September 26, 2014 ...
(Date:9/19/2014)... (PRWEB) September 19, 2014 Cereal ... food industries will gather October 5–8, 2014, for ... Rhode Island Convention Center in Providence, Rhode Island. ... the work of food scientists, chemists, microbiologists, nutritionists, ... and food production management. To date, 1,000 attendees ...
(Date:9/19/2014)... The new , Healthcare and Life ... today on PharmaBoardroom.com for free download , ... the Spanish economy in 2009, and the austerity measures that ... pragmatic in times of trouble. "Despite the crisis, ... European market, and the general conditions to invest are favorable ...
(Date:9/18/2014)... About POCT POCT, ... clinical laboratory. It helps in making fast clinical ... medication. POCT is gaining popularity due to the ... as diabetes, heart disease, and obesity. It is ... to minimize errors during the diagnosis of patients. ...
Breaking Biology Technology:Pfenex to Present at Upcoming Industry Conference on September 26 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 3PharmaBoardroom Releases New Spain Pharmaceuticals Report 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 3POCT Market in China 2014-2018 2
... , CHICAGO, Aug. 26 Ajinomoto ... by adding food product development capabilities. The Application Center North ... , , The ACNA mission is to develop ... food technology applications will be the initial focus of the Center: ...
... , SAN MARINO, Calif., Aug. 26 Viral ... for HIV/AIDS, autoimmune diseases, and cancer, is issuing this announcement in ... August 25 and August 26, and an article published on ... Viral Genetics, Inc. (VRAL)," on August 25. , , ...
... , , , ... SGP ), today announced that the European Medicines Agency ... Application (MAA) for a fixed-dose combination of mometasone furoate and ... 12 years of age and older. The company also ...
Cached Biology Technology:Viral Genetics Responds to Rumors of Imminent FDA Filing and Third Party Press Releases 2Viral Genetics Responds to Rumors of Imminent FDA Filing and Third Party Press Releases 3Schering-Plough Announces Two European Filings 2Schering-Plough Announces Two European Filings 3Schering-Plough Announces Two European Filings 4Schering-Plough Announces Two European Filings 5Schering-Plough Announces Two European Filings 6Schering-Plough Announces Two European Filings 7Schering-Plough Announces Two European Filings 8
(Date:9/18/2014)... acquire and creatively manipulate spoken language is unique to ... 6 million years of human evolution to make this ... candidate gene we now have," says Wolfgang Enard, Professor ... efforts to understand the molecular biological basis of language ... results of his latest study, undertaken in collaboration with ...
(Date:9/18/2014)... 18, 2014Many Americans across racial and ethnic groups ... impact on their day-to-day life, more so than ... and speech (57% of African-Americans, 49% of non-Hispanic ... When asked which disease or ailment is the ... first among African-Americans followed by AIDS/HIV. Hispanics ...
(Date:9/18/2014)... The global cycles of carbon and nitrogen comprise ... human and environmental systems have caused imbalances and ... greenhouse gases (GHGs). Increased GHGs result in climate ... terrestrial ecosystems, oceans and the atmosphere. Understanding these ... exciting branch of environmental science during the past ...
Breaking Biology News(10 mins):Language evolution: Quicker on the uptake 2Language evolution: Quicker on the uptake 3Americans rate losing eyesight as having greatest impact on their lives 2Americans rate losing eyesight as having greatest impact on their lives 3Greenhouse gas research enters a new era 2
... improve blood flow in diseased arteries, reducing the risk of ... published today reveals. , The finding is a significant development ... Care, hopes to use the drug as part of a ... Schmidt, Director of the Centre for Vascular Health and his ...
... women with serious depression and healthy women in a ... , The study adds further evidence that depression has ... specifically in the endogenous opioid system that is a ... system. The findings also show significant variation between individuals ...
... have a significantly increased risk of having autism spectrum ... 30 years, according to an article in the September ... JAMA/Archives journals. , Autism is characterized by social and ... background information in the article. Autism and related ...
Cached Biology News:Heart smart: new drug improves blood flow 2New brain-chemistry differences found in depressed women 2New brain-chemistry differences found in depressed women 3Older fathers more likely to have autistic children 2
Mycoplasma and virus tested...
... MagicMark XP Western Protein Standards are ... protein molecular weight estimation directly on ... an IgG binding site, allowing direct ... used to detect your target protein ...
... Clone/PAD: 3F10B10. Immunogen: Synthetic ... region of JAB1 (Jun activation ... Specificity: Specific for the JAB1 ... controls: 293T Jurkat cell lysates). ...
...
Biology Products: