Navigation Links
Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

o plain glass slides. Slides were stored at -70 oC until use.
NOTE: Slides may be stored at -70oC for up to six months.

2. Slides were stained with hematoxylin and eosin, and dehydrated per the following protocol.
1) Defrost slides briefly, 30 seconds or less.
2) Fix with 70% ethanol for 30 seconds
3) Wash with DEPC-treated distilled water for 30 seconds.
4) Stain with Mayers hematoxylin (DAKO, Cat. # S3309) for 30 seconds.
5) Wash with DEPC-treated distilled water for 30 seconds.
6) Wash with Bluing reagent (1X Scotts tap water; Sigma, Cat. # S5134) for 30 seconds.
7) Dehydrate with 70% ethanol for 30 seconds.
8) Dehydrate with 95% ethanol for 30 seconds.
9) Stain with Eosin (Sigma, Cat. # E6003) for 30 seconds.
10) Place the slides to 75% ethanol for 30 seconds.
11) Place the slides to 95% ethanol for 30 seconds.
12) Place the slides to 100% ethanol for 30 seconds.
13) Place the slides to xylene for 5 minutes.
14) Remove slides from xylene and air dry for 5 minutes.

ISOLATION OF SPECIFIC CELL POPULATION BY LCM ANALYSIS
LCM was performed using a PixCell II system (Arcturus, Cat# LCM1106) using CapSure Macro LCM caps (Arcturus, Cat# LCM0211). Pure luminal epithelium of implantation or interimplantation sites was isolated using LCM (Figure 1A), filling an entire CapSure Macro cap with cells of interest.


RNA ISOLATION AND AMPLIFICATION
1. Total cellular RNA was extracted from captured cells immediately after microdissection using the PicoPure RNA Isolation Kit (Arcturus, Cat. # KIT0202).
a) 50 l extraction buffer was pipetted into a 0.5 ml microcentrifuge tube.
b) Placed the CapSure caps carrying the captured cells onto the microcentrifuge tube and invert
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Fractionating DNA Fragments Generated by Differential Display PCR
2. Identification of Differentially Expressed Gene Products with the CastAway System*
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
5. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
6. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
7. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
8. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
9. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. A New Lambda Vector for Mammalian Expression
Post Your Comments:
(Date:4/30/2015)... Cambridge, MA & Budapest, Hungary (PRWEB) April 30, ... the only web-based electronic lab notebook with integrated ... leader in providing chemistry software solutions and consulting ... that led to the development of Labguru’s newest ... a set of deep domain features for biologists, ...
(Date:4/30/2015)... 2015 WuXi PharmaTech (Cayman) Inc. ("WuXi" or ... contract R&D services provider, today announced that its board ... dated April 29, 2015, from a consortium (the "Consortium") ... founder, chairman and chief executive officer of the Company, ... a transaction (the "Transaction") involving the acquisition of all ...
(Date:4/30/2015)... Sterlitech is proud to offer ... PX, PY, and PZ Polyacrylonotrile (PAN) membranes , ... Designed to be ideal for oil removal in ... critical process requirements where the presence of oils ... regulations on industrial wastewater discharge and quality requirements ...
(Date:4/29/2015)... , April 29, 2015 Cambrex Corporation (NYSE: ... be released on Friday, May 1, 2015 before the market ... The Company will host a conference call to discuss the ... When: , Friday, May 1, 2015 at ... , 1-888-468-2440 for U.S.1-719-325-2454 for International  Passcode:  7993632 Dial-in ...
Breaking Biology Technology:Labguru Integrates ChemAxon's Marvin JS, to Enhance Functionality for Chemists 2WuXi PharmaTech Announces Receipt of Proposal 2WuXi PharmaTech Announces Receipt of Proposal 3WuXi PharmaTech Announces Receipt of Proposal 4WuXi PharmaTech Announces Receipt of Proposal 5WuXi PharmaTech Announces Receipt of Proposal 6Sterlitech Offers New Ultrafiltration Membranes 2
... 2010 / PRNewswire/ -- AltheaDx, Inc. – ... of a strategic partnership with Compendia Bioscience to ... validation and bioinformatics. The two companies will jointly ... biomarker discovery and subsequent development of tests that ...
... electrical properties, carbon nanotubes are attractive building ... sensors, logic devices, and memory elements. ... arrays of individual carbon nanotubes and the ... large-scale commercial use. Now, researchers at ...
... BioSciences, Inc. (Nasdaq: SGMO ) announced today the ... correct a disease gene in an animal using systemic delivery ... four made by Sangamo,s collaborators at the 52nd Annual Meeting ... held in Orlando, Florida.   Abst.# 647 ...
Cached Biology Technology:AltheaDx and Compendia Bioscience Launch the Breast Cancer Segregation Panel™ 2Using new materials to make more reliable nanoelectromechanical systems 2Sangamo BioSciences Announces Data Presentation of First In Vivo Demonstration of ZFN-mediated Gene Correction Via Systemic Delivery at American Society for Hematology Meeting 2Sangamo BioSciences Announces Data Presentation of First In Vivo Demonstration of ZFN-mediated Gene Correction Via Systemic Delivery at American Society for Hematology Meeting 3Sangamo BioSciences Announces Data Presentation of First In Vivo Demonstration of ZFN-mediated Gene Correction Via Systemic Delivery at American Society for Hematology Meeting 4
(Date:4/9/2015)... , April 9, 2015 Synaptics Inc. (NASDAQ: ... solutions, announced today that it will report financial results ... April 23, 2015, after the close of market. The ... and investors at 2:00 p.m. PT (5:00 p.m. ET), ... To participate on the live call, analysts and ...
(Date:4/2/2015)... 2, 2015 According to preliminary ... to somewhat higher than 125 MSEK. This exceeds the ... 2014 that revenues for Q1 2015 would be in ... for Q4 2014. The operating result for the first ... complete interim report will as previously communicated be published ...
(Date:3/31/2015)...   Guidepoint , a leading global research services ... Management TRACKER, part of the Guidepoint TRACKER ... medical device and therapeutics markets. The Post-Surgical Pain Management ... market share, and adoption rates across three major modalities ... The Post-Surgical Pain Management TRACKER will provide ...
Breaking Biology News(10 mins):Synaptics to Report Third Quarter Results on April 23 2Fingerprint Cards Revises Revenues for Q1 2015 2Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3
... Scientists from the Universit de Montral and McGill University ... chemical reactions within the human body, to become highly ... a new study published in The Journal of ... an enzyme structure," says Joelle Pelletier, a professor at ...
... nearly 50 years, a population of a nearly extinct ... Forest,s San Jacinto Wilderness. Biologists from the U.S. Geological ... and scientists from the San Diego Natural History Museum ... rare mountain yellow-legged frog in the San Jacinto Wilderness ...
... University of British Columbia researchers are part of an ... land plants, a step they hope will lead to ... ultimately boost conservation efforts. Barcodes based on portions ... on products have already emerged as a viable ...
Cached Biology News:Biologists rediscover endangered frog population 2Biologists rediscover endangered frog population 3Biologists rediscover endangered frog population 4UBC researchers help push for standard DNA barcodes for plants 2
Amino-acids and Derivatives Rabbit Anti-conjugated L-isoleucine Polyclonal Antibody...
Anti-MMP-25 (Membrane-type MMP-6, Mt6MMP), hinge region; rabbit host...
PPIF Antibody...
Rabbit polyclonal to FLIP ( Abpromise for all tested applications). entrezGeneID: 8837 SwissProtID: O15519...
Biology Products: