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Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System

ppeared to be stable for at least 17 h as demonstrated by the curve in Figure 4.

Z’ analysis
Finally, two independent Z’ analysis (4) evaluations were performed, each using 48 replicate values for "total" and NSB wells. DMSO was present at a final concentration of 1% (v/v) in each well. A mean Z’ value of 0.80 was generated. Figure 5 is representative of the data obtained. This is well within the acceptable Z’ value range (0.5–1.0) and confirmed the robustness of the assay.


Conclusion
The adrenergic α2c receptor binding assay has been successfully miniaturized to a 384-well format using the LEADseeker Multimodality Imaging System and is suitable for adaptation to automated screening formats. The assay is tolerant to DMSO up to a concentration of 1% (v/v), robust; achieving a mean Z’ value of 0.80, and has a stability window of at least 17 h.


References
1. Ruffolo, R. R. Jnr et al. Annual Review Pharmacological Toxicology 32, 243–279 (1993).

2. Hein, L. et al. Nature 402, 181–184 (1999).

3. Bylund, D.B. et al. Pharmacological Reviews 46, 121–136 (1994).

4. Zhang, J. et al. Journal Biomolecular Screening 4 (2), 67–73 (1999).



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