Navigation Links
Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System

with dilutions of [0-methyl-3H]Rauwolscine to give a range of concentrations from 0.07 to 8.8 nM in the wells. Plates were incubated for 5 h prior to imaging. Figure 1 shows the saturation curve, which was fitted using nonlinear regression with the data analysis software package GraphPad Prism v4.0. Over two experiments the mean Kd value was determined to be 1.3 nM (95% confidence intervals 0.8 to 1.9 nM), estimated directly from the curve. Figure 1 is representative of the data obtained.

DMSO tolerance
Dilutions of DMSO were prepared in assay buffer to give a range of concentrations from 0.25% (v/v) to 5% (v/v) in the well. The results shown in Figure 2 indicate the assay was tolerant to DMSO up to a final concentration of 1% (v/v).

IC50 determination
Competitive binding of 1.3-nM [0-methyl-3H]Rauwolscine with rauwolscine (Fig 3A) and prazosin (Fig 3B) was assessed and the IC50 value of each ligand calculated. Final concentrations in the wells were from 0.0004 to 4000 nM and 0.25 to 20000 nM respectively. DMSO was present at a final concentration of 1% v/v in each well.

Over two experiments, the mean rauwolscine IC50 value was determined to be 3.1 nM (95% confidence interval range 2.7–3.7 nM) and the mean Ki value was 1.6 nM (95% confidence intervals 1.3–1.8 nM). The mean prazosin IC50 value was determined to be 430 nM (95% confidence interval range 336–535 nM) and the mean Ki value was 215 nM (95% confidence intervals 168–267 nM).

Time course
A time course was performed using standard reagent concentrations detailed in the protocol with an increased incubation time of 17 h. The assay a
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
6. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
7. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
8. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
9. Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
10. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
11. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
Post Your Comments:
(Date:9/13/2014)... Phoenixville, PA (PRWEB) September 13, 2014 ... medical device innovations, is proud to announce the successful ... Health Network bariatric surgeons Leonardo Claros, MD, FACS, FAMBS, ... ViSiGi 3Dâ„¢ Sleeve Gastrectomy Calibration System. , This ... a postgraduate course of more than four hundred surgeons ...
(Date:9/12/2014)... 12, 2014 Advancing the front ... of chemical and biological threats is the mission ... Polytechnic Institute (WPI) and the Georgian National Academy ... for Peace and Security Programme of the North ... Advanced Research Workshop series, the event is titled ...
(Date:9/12/2014)... 12, 2014 On Thursday, September 11, ... Dow Jones Industrial Average finished the day 0.12% lower at ... The gains were broad based as seven out of ten ... Care Sector Index ended the day at 741.83, down 0.33%, ... month. Investor-Edge has initiated coverage on the following equities: Exelixis ...
(Date:9/11/2014)... cells (hiPSCs), researchers have gained new insight into ... patterns of neuronal signaling associated with this disease. ... the hiPSCs of healthy individuals and of patients ... stem cells to release neurotransmitters, such as dopamine, ... linked to various disorders. By discovering a simple ...
Breaking Biology Technology:Boehringer Laboratories, Inc. Announces Live Surgery Using Innovative System to Calibrate Sleeve Gastrectomy 2Worcester Polytechnic Institute to Lead Scientific Workshop on Nanoscale Defenses for Biological and Chemical Threats 2Worcester Polytechnic Institute to Lead Scientific Workshop on Nanoscale Defenses for Biological and Chemical Threats 3Technical Coverage on Biotech Stocks - Exelixis, Arena Pharma, Novavax, Dendreon, and Amicus Therapeutics 2Technical Coverage on Biotech Stocks - Exelixis, Arena Pharma, Novavax, Dendreon, and Amicus Therapeutics 3Technical Coverage on Biotech Stocks - Exelixis, Arena Pharma, Novavax, Dendreon, and Amicus Therapeutics 4Technical Coverage on Biotech Stocks - Exelixis, Arena Pharma, Novavax, Dendreon, and Amicus Therapeutics 5Stem cells help researchers understand how schizophrenic brains function 2
... /Xinhua-PRNewswire-FirstCall/ --,China-Biotics, Inc. (OTC Bulletin Board: CHBT) ... in the manufacture,research, development, marketing and distribution ... a new bilingual corporate Web site under,the ... The new Web site was created ...
... of tightly packed nanostructures that resemble tiny nails, University ... have created a material that can repel almost any ... liquid on the surface slips past the heads of ... the surface completely. , The new material, which was ...
... Mass., Jan. 29 Thermo Fisher,Scientific Inc. (NYSE: ... it will present at the 2008 Merrill Lynch Global ... Grand Hyatt,Hotel in New York City. The company will ... Marijn E. Dekkers, president and chief executive officer ...
Cached Biology Technology:China-Biotics, Inc. Launches English / Chinese Corporate and Investor Relations Web Site 2Thermo Fisher Scientific to Present at the 2008 Merrill Lynch Global Pharmaceutical, Biotechnology & Medical Device Conference 2
(Date:9/15/2014)... released today by the National Academy of Sciences, ... and strategies for the program and will guide ... years (2015-2020). In addition, the program announced ... in 2015, will include exploratory grants, early-career research ... $500 million, 30-year program to be run by ...
(Date:9/15/2014)... in the oceans, there must be enough dissolved ... extreme oxygen-starvation produces "dead zones" that decimate marine ... oxygen levels decline, energy is increasingly diverted away ... in impacts on the ecology and biogeochemistry of ... oxygen minimum zones (OMZs) have expanded due to ...
(Date:9/15/2014)... who transplanted combinations of wild, domesticated, and ... to new environments found that within 5 ... foreign genes from wild populations that hybridized ... Applications study provides evidence that natural ... that hybridized populations experience as a result ...
Breaking Biology News(10 mins):NAS Gulf Research Program announces strategic vision, initial opportunities 2NAS Gulf Research Program announces strategic vision, initial opportunities 3NAS Gulf Research Program announces strategic vision, initial opportunities 4Decoding virus-host interactions in the oxygen-starved ocean 2Decoding virus-host interactions in the oxygen-starved ocean 3Decoding virus-host interactions in the oxygen-starved ocean 4Decoding virus-host interactions in the oxygen-starved ocean 5
... undergo surgery for breast cancer followed by radiation ... be corrected through reconstructive surgery. Researchers at the ... bioengineers at Carnegie Mellon University, have developed a ... as an artificial tissue filler after surgery and ...
... Researchers at the McKusick-Nathans Institute of Genetic Medicine at ... way of analyzing what many have termed "junk" DNA ... they have found that these control regions from different ... , The study will be published online at Science ...
... system response to foreign DNA, suggesting that DNA viruses ... School of Medicine researchers report this week in Immunity. ... be used to detect invasive bacteria in addition to ... in the Section of Immunobiology and lead author of ...
Cached Biology News:Chemotherapy gel may fight breast cancer and reduce breast deformity 2Junk DNA may not be so junky after all 2Junk DNA may not be so junky after all 3Immune system response to viral DNA is unique 2
... Protein A and Protein G bind specifically ... Protein A and Protein G conjugates are ... immunoglobulins (antibodies) and immunoglobulin subtypes from serum, ... other biological fluids. These reagents are also ...
... The SYS203 is a complete, ... measurement in small samples, as is ... system is designed around Instechs miniature ... of an inch in diameter (3.2 ...
... family of human cullin genes (CUL-1, -2, -3, -4a, ... gene. Cullin-3 is proposed to be a part of ... cyclin E and targets it for ubiquitination, which leads ... activator of cdk2, which leads the cell to S ...
... MetaVue Imaging System is the cost-effective solution ... commands tailored to common research imaging tasks, ... analysis and simple image analysis. Compatibility ... library means that MetaVue has the power ...
Biology Products: