Navigation Links
Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System

Key words: α2c receptor GPCR receptor binding assay LEADseeker SPA Imaging Beads


The α2-adrenergic receptors are G protein-coupled receptors that play a key role in the control of numerous physiological functions such as renal Na+ re-absorption, insulin secretion, platelet aggregation, and neurotransmitter release at sympathetic nerve endings (1). They consist of three highly homologous subtypes encoded by distinct genes: α2a, α2b, and α2c. Both α2a and α2c subtypes are required for normal presynaptic control of neurotransmitter release from sympathetic nerves from the heart and from central noradrenergic neurons. α2a adrenergic receptors inhibit transmitter release at high stimulation frequencies whilst the α2c subtype modulates neurotransmission at lower levels of nerve activity (2). The α2c receptor is distinguishable from α2a and α2b receptors by its sensitivity to prazosin (3).

This application note describes a 384-well adrenergic α2c receptor binding assay developed using the LEADseeker™ Multimodality Imaging System.


Materials
Products used

LEADseeker Multimodality Imaging System 18-1140-71

Wheat Germ Agglutinin (WGA) YOx RPNQ0270
SPA Imaging Beads

[0-methyl-3H]Rauwolscine TRK685


Other materials required
Human recombinant adrenergic α2c receptor membrane preparation (Euroscr
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
6. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
7. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
8. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
9. Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
10. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
11. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
Post Your Comments:
(Date:1/15/2014)... the device," Nobel laureate Herbert Kroemer famously observed, ... at the junctures where layers of different materials ... interfaces between layers of metal oxides are becoming ... as spintronics, high-temperature superconductors, ferroelectrics and multiferroics. Realizing ...
(Date:1/15/2014)... 15, 2014 Look inside the new ... for the lab, from fluid handling to instruments to ... ship when you order. , Preferred Solutions features ... from the L/S® model for precise flow control and ...
(Date:1/15/2014)... 2014 This webinar will focus on ... safety assessment in biosimilars. , Regulatory frameworks are evolving ... development, however the complex nature of biopharmaceuticals makes the ... efficacy extremely challenging. Based on the specific aspects of ...
(Date:1/15/2014)... Rochester, NY (PRWEB) January 15, 2014 The ... cause a major disease. One of these latent viruses is ... is rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... the theory, a study found that RA patients have high ...
Breaking Biology Technology:A deeper look at interfaces 2Cole-Parmer Begins 2014 with the Release of Preferred Solutions 2Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3
... LYNBROOK, N.Y., June 29 BioSpecifics Technologies Corp. (Nasdaq: ... collagenase-based products, announced today that it was added to the ... of the U.S. markets on June 29, 2009. The Russell ... to be a comprehensive representation of the investable U.S. equity ...
... Kendle (Nasdaq: KNDL ), a leading, ... of Jarrod Pontius to the position of Chief Legal ... leadership for all legal services, corporate governance and general ... based in Kendle,s global headquarters in Cincinnati and will ...
... , , , BATESVILLE, Ind., June ... the sale of Hill-Rom,s patents and intellectual property relating to ... KCI ). Hill-Rom,s NPWT portfolio included U.S. ... components, protocols, and related therapies. The transaction is effective immediately. ...
Cached Biology Technology:BioSpecifics Technologies Corp. Added to Russell 3000 and Russell 2000 Indexes 2Kendle Names Jarrod Pontius Chief Legal Officer 2Hill-Rom Announces Sale of Negative Pressure Wound Therapy Intellectual Property 2Hill-Rom Announces Sale of Negative Pressure Wound Therapy Intellectual Property 3
(Date:4/17/2014)... Texas Medical Branch at Galveston are the first ... for cervical cancer should begin colorectal cancer screening ... researchers, finding a high incidence of secondary colorectal ... offer new recommendations that the younger women in ... years after their initial cervical cancer diagnosis instead ...
(Date:4/17/2014)... from Charles Darwin, whose ideas in turn were ... 19th century, a period when landowners vigorously pursued ... Darwin that we inherit the ideas that domestication ... and total human control over breeding and animal ... setting has been applied too broadly in time ...
(Date:4/16/2014)... cannot lift your upper eyelid. You walk through life with your ... are rolled down in the eye socket. Obviously, such a condition ... head. In order to correct this condition, one would need to ... April 16 print issue of the journal Neuron , University ...
Breaking Biology News(10 mins):Radiation therapy for cervical cancer increases risk for colorectal cancer 2The story of animal domestication retold 2The story of animal domestication retold 3The story of animal domestication retold 4Researchers track down cause of eye mobility disorder 2
... City once topped lists of places with the worst air ... improved the situation, tiny particles called aerosols still clog the ... other institutions have sorted through the pall that hangs over ... haze and chart daily patterns of changes to the mix. ...
... SEPTEMBER 2, 2008 Varying the rate of crop production ... as farmers have long observed differences in crop yield ... of spatial yield information from combines equipped with yield ... So, optimizing inputs to match yield potential ...
... visitor might blink twice at the sight of a ... the northern edge of Arizona State University (ASU), is ... canals and dams combining flood control, water delivery, recreational ... and economics in the area. What are ...
Cached Biology News:Smoke smudges Mexico City's air, chemists identify sources 2Smoke smudges Mexico City's air, chemists identify sources 3A little nitrogen can go a long way 2A little nitrogen can go a long way 3Evolving designer ecosystem sheds light on unintended consequences 2Evolving designer ecosystem sheds light on unintended consequences 3Evolving designer ecosystem sheds light on unintended consequences 4
... For use in hybridization incubators ... and GL 45 size PP plug-seal ... seal. Available in three lengths to ... plug-seal caps and O-rings available separately. ...
... Rapid, efficient extraction of DNA from agarose gels ... of DNA fragments of 150 bp to > 12,000 ... GelMelt solution to dissolve the gel slice, followed by ... a spin column format. After a wash step, the ...
Fujifilm BAS-1800II provides the ideal configuration for a low-cost imager that requires only a small amount of space in the laboratory. The small imager for accurate high-throughput screening...
When added to StemSep T Cell Enrichment cocktail, human TCRab TAC is designed to deplete human TCRab from samples to isolate gd T cells....
Biology Products: