Navigation Links
Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System

Key words: NK2 • GPCR receptor binding assay LEADseeker SPA Imaging Beads


Neurokinins, also known as tackykinins, are a family of small neuropeptides. Members of this family include peptides, substance P (SP), neurokinin A (NKA), neurokinin B (NKB), and the recently discovered hemokinin-1 (1). Neurokinins are widely distributed throughout the central and peripheral nervous system where they act via G protein-coupled receptors (NK1, NK2, and NK3 receptors) as neurotransmitters and neuromodulators. They are also considered key mediators in the communication between neurons (particularly sensory neurons) and effector cells (e.g., smooth muscle, glands, and immune cells), a role that is significant to the pathophysiology of a wide variety of diseases including gastrointestinal disorders, emesis, chronic pain, depression, and asthma (2). Members of the neurokinin family favor specific receptors. SP, for instance, shows greatest affinity for NK1 receptors, NKA for NK2, and NKB for NK3 (3). Of these receptors, NK2 receptors are thought to be expressed mainly on smooth muscle cells (4). Additionally, NKA and its truncated form, NKA (4-10), are potent contractors of human colon circular muscle, an action mediated exclusively via NK2 receptors (5).

This application note describes a 384-well NK2 neuropeptide receptor binding assay developed using LEADseeker™ Multimodality Imaging System. The miniaturized assay is robust, achieving a Z’ value of 0.62 and a stability window of 18 h. These factors make the assay suitable for adaptation to automated screening formats.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

Wheat Germ Agglutinin (WGA)-PEI Type A RPNQ0287
PS S
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
6. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
7. Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System
8. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
9. Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
10. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
11. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
(Date:9/1/2015)... ... September 01, 2015 , ... Leading CEOs, CSOs, CMOs, ... management space join prominent academics and key investors for Neuro Advance Boston, October ... in Boston. , Neuro Advance Boston focuses on the latest developments and ...
(Date:9/1/2015)... ... September 01, 2015 , ... Industry ... Development, Asia Pacific. In this newly-created position, Gibbs will lead iLab’s business operations ... iLab’s suite of tools. This entails not only introducing iLab to potential partners ...
(Date:9/1/2015)... (PRWEB) , ... September 01, 2015 , ... ... announced the release of a groundbreaking new version of its flagship DNA design ... methodology breaks down complex DNA sequences into numerous genetic parts, which in turn ...
(Date:8/31/2015)... 31, 2015 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or ... orders are for air emission abatement projects in ... a large order for a municipality in the ... a competitor,s system that failed to meet the customer,s requirements. This ... said Derek S. Webb , President and Chief Executive ...
Breaking Biology Technology:Neuro Advance Boston Brings Together Leading Neuroscience Experts on October 14 at Harvard Medical School 2iLab Solutions Hires Gerard Gibbs, Director of Business Development, Asia Pacific 2iLab Solutions Hires Gerard Gibbs, Director of Business Development, Asia Pacific 3GenoCAD Releases Combinatorial Design Tool to Optimize Protein Expression and Streamline DNA Synthesis Orders 2
... CALGARY, Nov. 29 /PRNewswire-FirstCall/ - Oncolytics Biotech ... Coffey, Chief,Scientific Officer of Oncolytics, is scheduled ... 2007 entitled "Large Scale Production Process for,the ... Informa Life,Sciences, 3rd Annual Vaccine Production Conference ...
... properties of a commonly used blue dye could revolutionise ... Advanced Materials. , Scientists have demonstrated that they can ... Phthalocyanine, or MPc, with the use of magnetism. ... researchers claim that the ability to control the magnetic ...
... Calif., Nov. 28 Bionovo, Inc.,(Nasdaq: BNVI ), a ... in the areas of women,s health and cancer, is scheduled ... Conference., The presentation by Tom Chesterman, SVP and CFO ... at 1:30 p.m. local time at the,Millennium Broadway Hotel in ...
Cached Biology Technology:Oncolytics Biotech Inc. to Present at Annual Vaccine Production Conference 2Blue dye could hold the key to super processing power 2Blue dye could hold the key to super processing power 3
(Date:8/6/2015)... Calif. , Aug. 6, 2015  Today, ... will visit Crossmatch ™ to learn firsthand ... and protect data. Crossmatch is a world leader ... technologies and expertise are helping federal agencies and ... and security.   "The vast majority ...
(Date:8/5/2015)... , Aug. 5, 2015 The biosensors market ... in applications, penetration into newer sectors, and development of ... global biosensors space has seen the entry of multiple ... so far. (Photo - http://photos.prnewswire.com/prnh/20150805/255570 ) ... Analysis of the Global Biosensors Market ( ...
(Date:7/31/2015)... de julio de 2015 BGI llevará a cabo ... del 22 al 25 de octubre de 2015, en ... conferencia celebra su décimo aniversario este año. Desde su ... de las reuniones anuales más influyentes del mundo en ... las conferencias científicas más dinámicas, entusiastas y amenas. ...
Breaking Biology News(10 mins):Rep. Honda to Visit Crossmatch in Redwood City 2Innovative Biosensors Incite Use in Non-Traditional Applications 2Innovative Biosensors Incite Use in Non-Traditional Applications 3Innovative Biosensors Incite Use in Non-Traditional Applications 4La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3
... protect nerves are the likely origin of the Devil Facial ... population, an international team of scientists has discovered. ... affects only Tasmanian devils and was first reported in 1996. ... The disease is characterised by large tumours, mostly on the ...
... FL Scientists from The Scripps Research Institute have ... infectious protein devoid of DNA or RNA that can ... The study from Scripps Florida in Jupiter ... at the protein level and, through natural selection, these ...
... in the remains of long-dead plants, animals, or humans allows ... studies of this kind on ancestral members of our own ... ancient DNA from modern-day human DNA contamination. Now, research by ... Leipzig, published online on December 31st in Current Biology ...
Cached Biology News:Nervous culprit found for Tassie devil facial tumor disease 2Scripps Florida scientists show 'lifeless' prions capable of evolutionary change and adaptation 2Scripps Florida scientists show 'lifeless' prions capable of evolutionary change and adaptation 3Scripps Florida scientists show 'lifeless' prions capable of evolutionary change and adaptation 4Using modern sequencing techniques to study ancient modern humans 2
... Timp-4 has been reported in heart ... fibroblasts in cell culture conditions. ... TPA stimulates production of TIMP-4 in ... protein levels produced often require concentration ...
Goat polyclonal to HSPC150 ( Abpromise for all tested applications). entrezGeneID: 29089...
Anti-MMP-13 Collagenase-3, amino terminal end of active enzyme; rabbit host...
...
Biology Products: