Navigation Links
Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System

Key words: NK2 • GPCR receptor binding assay LEADseeker SPA Imaging Beads


Neurokinins, also known as tackykinins, are a family of small neuropeptides. Members of this family include peptides, substance P (SP), neurokinin A (NKA), neurokinin B (NKB), and the recently discovered hemokinin-1 (1). Neurokinins are widely distributed throughout the central and peripheral nervous system where they act via G protein-coupled receptors (NK1, NK2, and NK3 receptors) as neurotransmitters and neuromodulators. They are also considered key mediators in the communication between neurons (particularly sensory neurons) and effector cells (e.g., smooth muscle, glands, and immune cells), a role that is significant to the pathophysiology of a wide variety of diseases including gastrointestinal disorders, emesis, chronic pain, depression, and asthma (2). Members of the neurokinin family favor specific receptors. SP, for instance, shows greatest affinity for NK1 receptors, NKA for NK2, and NKB for NK3 (3). Of these receptors, NK2 receptors are thought to be expressed mainly on smooth muscle cells (4). Additionally, NKA and its truncated form, NKA (4-10), are potent contractors of human colon circular muscle, an action mediated exclusively via NK2 receptors (5).

This application note describes a 384-well NK2 neuropeptide receptor binding assay developed using LEADseeker™ Multimodality Imaging System. The miniaturized assay is robust, achieving a Z’ value of 0.62 and a stability window of 18 h. These factors make the assay suitable for adaptation to automated screening formats.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

Wheat Germ Agglutinin (WGA)-PEI Type A RPNQ0287
PS S
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
6. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
7. Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System
8. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
9. Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
10. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
11. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System

Post Your Comments:
(Date:11/19/2009)..., , COPENHAGEN, Nov. 19 Dan... Inbicon cellulosic ethanol plant in Kalundborg, D... one of the world,s first and largest 2nd generati... Accellerase enzymes are used in the plant,s innov...or, a Division of Danisco, was the first to market...
(Date:11/19/2009)..., , BETHESDA, Md., Nov. 19 /PRNewswire-Fir...an innovator in biotechnology for diabetes therapy...lting services to food, supplement, biotechnology ...results of Tuesday,s Annual Meeting of Shareholder...ncorporated (Nasdaq: SPEX ) elected the following...
(Date:11/19/2009)..., World,s Top Gene Synthe...nsortium (IGSC) to Coordinate Best Practices in Ri... Percent of Worldwide Gene Synthesis Capacity , ...- Five of the world’s leading gene synthesis...l apply a common screening protocol to promote bio...
(Date:11/18/2009)..., , Security personnel need to be able to find ...ontact with them. To aid such searches, the Nation...ith support from the Department of Homeland Securi...al, Standard Reference Material (SRM) 2905, Trace ...laboratory assay methods, the SRM will be helpful ...
Breaking Biology Technology:DONG Energy and Inbicon Inaugurate One of the World's Largest Demo Plants for 2nd Generation Biofuel 2Spherix Announces Results of Annual Shareholders Meeting 2Spherix Announces Results of Annual Shareholders Meeting 3Spherix Announces Results of Annual Shareholders Meeting 4World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 2World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 3World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 4New NIST trace explosives standard slated for homeland security duty 2
...ulator that could also help expand the use of virt...monstrated on Wednesday at Chippewa Valley Techni...er, which was co-developed by the visual technolog...s lets students gain hours of welding experience ...llowing detailed critiques. , ,"In a nutshell, it ...
...a local software and consulting firm, has teamed u...w geographic information systems application, the ...ivX Integrator, allows organizations to create and...ork or the Internet. , ,"We understand the challen...distribute data from several different, separate d...
...of anti-spam equipment and services provided by Gr...r small businesses. MailFoundry Hosted starts at $...he service, a company would route its incoming e-m... then sent back to the company,s mail accounts. In...ilFoundry also offers a rack-mounted server that c...
Other Biology Technology:Virtual-reality welding simulator an opportunity for Chippewa Valley college 2
(Date:11/23/2009)...erman . , A bit of imagination on the part o...It could help to add data from areas where the ins...so constructively. In order to infer missing data ... imagination, physicists at the Max Planck Institu...atial perception called information field theory. ...
(Date:11/23/2009)...erman . , Of all global carbon dioxide emiss...where it contributes to global warming. The remain...stems such as forests, grasslands and peat-lands. ...restrial ecosystems is considered one of the main,...ange. However, new greenhouse gas bookkeeping has ...
(Date:11/22/2009)...ts have inventoried an astonishing abundance, dive...ve never known sunlight - creatures that somehow m...00 meters (~3 miles) below the ocean waves. , R...nguard technologies, animals known to thrive in an...rse collection of species ranging from crabs to sh...
Breaking Biology News(10 mins):Visual assistance for cosmic blind spots 2Visual assistance for cosmic blind spots 3Visual assistance for cosmic blind spots 4Intensive land management leaves Europe without carbon sinks 2Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 2Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 3Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 4Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 5Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 6Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 7Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 8Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 9Mercy Health Plans Launches MercyOne Connect 28TM 29 49874 1Mercy Health Plans Launches MercyOne Connect 28TM 29 49874 2TriLink BioTechnologies Inc Announces Sales and License Agreement With Glen Research Corporation for CleanAmp 28TM 29 Amidites 49872 1TriLink BioTechnologies Inc Announces Sales and License Agreement With Glen Research Corporation for CleanAmp 28TM 29 Amidites 49872 2ChromaDex Establishes a Blanket Purchase Agreement With the National Institutes of Health 49869 1ChromaDex Establishes a Blanket Purchase Agreement With the National Institutes of Health 49869 2
... key compounds that stimulate stem cell growth in ...tion for people affected by Parkinson,s disease, s...urological disorders. These findings, which appear... provide important clues as to which compounds may...ns, to regenerate and ultimately restore brain fun...
...rs to prevent tuberculosis in cattle is unlikely t...odroffe, an ecologist at the University ofCaliforn...cientific Group on Cattle TB. , In Britain, farmin... for 30 years over the merits of culling badgers t...ccasionally be transmitted to people. Farming mini...
...men and children the hardest. A joint study betwee...versity in Uganda has now produced some important ...lf in the placenta. , Plasmodium falciparium is th...aria parasites that infect man. It is particularly... pregnant women, with fatal consequences for both ...
Other Biology News:British cattle give TB to badgers, finds UC Davis expert 2New findings could lead to vaccine for severe malaria 2
Silencer Pre-designed siRNA, PAGE purified from Ambion
...ably amplify unknown genomic DNA using our patent...eady-to use components, at an extraordinarily comp...ded in each kit. The Genome Walking Kit can be use...A, isolating promoter and regulatory sequences cor...
Rabbit Anti-Human YIPF6 Polyclonal Antibody, Unconjugated from Atlas Antibodies
Calf serum from Equitech-Bio
Biology Products: