Navigation Links
Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System

Key words: H1 receptor • GPCR • receptor binding assay • LEADseeker • SPA Imaging Beads


Histamine is a biogenic amine that plays important pathophysiological roles in central and peripheral tissues. Generally released from stimulated mast cells, it contributes to several biological processes that characterize allergic responses (1).The function of histamine is mediated via four G protein-coupled receptors, H1, H2, H3 and H4 (2). H1 receptor antagonists are one of the most commonly used class of drugs used to relieve the symptoms of allergic diseases, such as allergic rhinitis, atopic dermatitis, psoriasis and allergic conjunctivitis (3). The H1 receptor is thought to be involved in mediating inflammatory responses via effects on cytokine production (4).

This application note describes a 384-well histamine H1 receptor binding assay developed using the LEADseeker™ Multimodality Imaging System.


Materials
Products used

LEADseeker Multimodality Imaging System 18-1140-71

Wheat Germ Agglutinin (WGA) YOx SPA RPNQ0270
Imaging Beads

[pyridinyl-5-3H]Pyrilamine TRK608


Other materials required
Human recombinant Histamine (Euroscreen, ES-390-M)
H1 receptor membrane preparation

Pyrilamine maleate salt (Sigma, P5514)

Triprolidine hydrochloride (Sigma, T6764)
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
6. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
7. Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System
8. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
9. Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
10. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
11. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
(Date:7/22/2014)... 22, 2014 Even within a phylum so full of ... among other arthropods for its cruelty -- at least to ... the fly is a most predatory sort of parasite. It ... a smear of larvae, and leaves its wicked brood to ... , None of this would be possible without the fly,s ...
(Date:7/22/2014)... Nuclear reactors are used to produce electricity ... chain reaction in nuclear reactors is used to create ... a power- generating turbine. Currently nuclear reactors are used ... production. Nuclear reactor market is expected to grow in ... nuclear reactor projects. , Early buyers will receive 10% ...
(Date:7/22/2014)... July 22, 2014 Two new members ... of Directors at its annual Shareholders Meeting on June ... Officer of The PFM Group, and Stephen T. Zarrilli, ... also announced the election of Michael A. DiPiano, Managing ... Chair and Richard P. Jaffe, Esq., of Duane Morris ...
(Date:7/22/2014)... en SHENZHEN, China , 22 juli ... Tech), een dochtermaatschappij van BGI, ,s wereld grootste organisatie ... aan van een nieuwe service ten behoeve van hele ... platform van Complete Genomics. In 2013 ... sector in hoog aanzien staat vanwege de in eigen ...
Breaking Biology Technology:Fly-inspired sound detector 2Fly-inspired sound detector 3Fly-inspired sound detector 4Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 3Two New Members Elected to University City Science Center Board of Directors 2Two New Members Elected to University City Science Center Board of Directors 3BGI Tech introduceert service op gebied van hele menselijke exoomsequencing op geavanceerd Complete Genomics-platform 2
... Inc. (the "Company",or "SulphCo") (Amex: SUF ), a ... oil, announced today the appointment of Fred,S. Zeidman to ... to have Mr. Zeidman join SulphCo,s Board of Directors,",said ... "The Company,will benefit from his extensive experience, both in ...
... August 28 Strong organic,growth continued in Q2 2008 supported ... diagnostic product based on,miRNA profiling remains on track for year ... by 139% on the year-earlier period to DKK 28 ... of 2008., - Product sales in Q2 2008 increased ...
... Volcano Corporation,(Nasdaq: VOLC ), a leading ... measurement (FM) and Optical Coherence,Tomography (OCT) products ... coronary and peripheral vascular disease, said today ... Weisel Partners 2008 Healthcare Conference on,Thursday, September ...
Cached Biology Technology:SulphCo Adds New Board Member 2Exiqon A/S: Interim Report for the Period 1 January - 30 June 2008 (Unaudited) 2Volcano Presentation at Thomas Weisel Conference to Be Webcast 2
(Date:7/22/2014)... Rochelle, NY -- Stem cells offer much promise for ... approaches stem cell survival is poor, limiting their effectiveness. ... the survival and optimize their therapeutic function after transplantation, ... Open Access , a peer-reviewed journal from Mary Ann ... the BioResearch Open Access website . ...
(Date:7/22/2014)... point, most kids will hear that drinking milk helps make their ... do these messages foster the idea that if something is good ... study in the Journal of Consumer Research , when children ... eat it. , "We predicted that when food is presented to ... a goal such as learning how to read or count, they ...
(Date:7/22/2014)... July 22, 2014 BGI Tech Solutions Co., ... world,s largest genomics organization, announced today the global ... service based on Complete Genomics, industry-leading platform. This ... SNP validation, and is available at reduced prices ... highly regarded in the industry for providing proprietary ...
Breaking Biology News(10 mins):BGI Tech launches human whole exome sequencing service on complete genomics' advanced platform 2
... chemical used to make food-storage containers, car bumpers and ... is the first definitive detection of the plastic ingredient ... A small amount of propylene was identified in Titan,s ... instrument measures the infrared light, or heat radiation, emitted ...
... Sept. 30, 2013 UC Irvine scientists Aaron Esser-Kahn, ... of the prestigious 2013 National Institutes of Health Director,s ... supports projects by early-career researchers that show potential to ... into new ways to improve human health. Esser-Kahn, ...
... ovarian cancer that has recurred after chemotherapy have survived for ... to new results to be presented today (Monday) at the ... is taken in pill form, is an inhibitor of a ... essential for tumour growth, and it is the first oral ...
Cached Biology News:NASA's Cassini spacecraft finds ingredient of household plastic in space 2UCI achieves rare trifecta: 3 scientists receive New Innovator Awards 2Biological therapy with cediranib improves survival in women with recurrent ovarian cancer 2Biological therapy with cediranib improves survival in women with recurrent ovarian cancer 3
...
...
MarrowMAX Bone Marrow Medium...
Sprague-Dawley rats, 7-8 weeks old, mixed gender; tissue is collected fresh, frozen on dry ice within minutes of sacrifice and stored at -20C; individual tissue weight approx. 0.05 gram....
Biology Products: