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Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System

There was an excellent correlation between the multiplex suspension assay and the ELISA (r2 = 0.960.99)
The CV was lower in the multiplex assay than in the ELISA
There was no loss of sensitivity when multiplexing
Fewer false positives were detected with the multiplex assay than with the ELISA
The Bio-Plex assay had equivalent specificity to that of the ELISA, and slightly higher sensitivity

Triton is a trademark of Union Carbide. xMAP is a trademark of Luminex Corporation. The Bio-Plex suspension array system includes fluorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.

Information in this tech note was current as of the date of writing (2004) and not necessarily the date this version (Rev B, 2005) was published.


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