Navigation Links
Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System

screen animals for multiple viruses simultaneously, greatly reducing the time and labor required to perform our assays while also conserving limited samples.


Methods
Viral Disruption for Conjugation
To develop a method for viral conjugation, we began with our whole-virus ELISA protocol, in which the virus is disrupted using SDS. For the first bead conjugation, SRV was disrupted with SDS. We also tried undisrupted whole virus and whole virus disrupted with Triton X-100 detergent. To compare the results obtained with the various disruption methods, the beads were run on the Bio-Plex system.

Optimizing Conjugation by Virus/Plasma Titration
After determining that we could successfully conjugate whole disrupted virus to the beads, the next step was to optimize the amount of virus for bead conjugation, as well as to determine the optimum plasma concentration. The protocol provided with the Bio-Plex amine coupling kit suggested using 512 g of protein for conjugation. We conjugated beads with 2, 5, 8, 10, and 12 g of virus (disrupted with Triton X-100), and then reacted the conjugated beads with both positive and negative plasma at dilutions of 1:50, 1:100, and 1:200.

ELISA Assay
ELISA assays followed conventional ELISA protocols.

Bio-Plex Assay
Multiplexing was performed according to the following protocol:
1. Prepare plasma samples.
2. Load beads into wells.
3. Wash beads.
4. Incubate plasma with antigen-conjugated beads.
5. Wash beads.
6. Incubate beads with phycoerythrin detection antibody (Figure 1).
7. Wash beads.
8. Read on Bio-P
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
5. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
6. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
7. Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System
8. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
9. Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
10. Mx4000 Multiplex Quantitative PCR System*,PPP
11. QIAGEN Multiplex PCR Handbook
Post Your Comments:
(Date:7/24/2014)... Three companies from the angelMD network were ... Competition. Out of 700 competition entrants, Chiron Health, Litesprite, ... round. In this round twenty teams are invited to ... mentoring from thought-leaders and medical entrepreneurs from all over ... commitment to the Livestrong vision around the Big C ...
(Date:7/24/2014)... Mich. , July 24, 2014  Neogen Corporation ... that future forecasts of its potential revenue from new ... call to investors and analysts on July 22, 2014, ... by analysts regarding this research. "It was ... questions about the potential of a new type of ...
(Date:7/24/2014)... 1000 balances – 4 steps – One Recommendation: ... and expertly narrows down the mass of product choices ... terminals. , First, select the working environment, then ... instead) and define the accuracy – it’s really that ... the list to models that fit best with the ...
(Date:7/24/2014)... 24, 2014 Draper University, a ... proud to announce that for 6 weeks this ... will converge on the Draper University campus in ... experiential program focused on entrepreneurship, building real-world skills, ... currently running its sixth cohort, continues to refresh ...
Breaking Biology Technology:3 angelMD Startups Make Livestrong Big C Semi-finals 2Neogen comments on SenesTech 2Summer Entrepreneurship Program Kicked Off by Draper University in Silicon Valley 2Summer Entrepreneurship Program Kicked Off by Draper University in Silicon Valley 3
... BRUNSWICK, N.J., Feb. 18 Senesco Technologies, Inc. ... financial results for the three months ended December 31, 2008. ... 2008 was $1,624,341, or $0.09 per share, compared with a ... three month period ended December 31, 2007. This increase ...
... a,biotechnology company focused on novel therapeutic and preventative,approaches ... of an EUR18M ($23M) Series B financing round ... operational and clinical level. Novartis Venture Fund,Fountain Healthcare ... all participated in the funding. , ...
... more light than heat. Engineers working in optical technologies ... with much greater speed than do electrical signals, says ... Bethlehem, Pa. If they are guided with sufficient precision ... can bring about applications in spectroscopy, sensing and medical ...
Cached Biology Technology:Senesco Technologies Reports Second Quarter Fiscal 2009 Financial Results 2Senesco Technologies Reports Second Quarter Fiscal 2009 Financial Results 3Senesco Technologies Reports Second Quarter Fiscal 2009 Financial Results 4Senesco Technologies Reports Second Quarter Fiscal 2009 Financial Results 5Senesco Technologies Reports Second Quarter Fiscal 2009 Financial Results 6Senesco Technologies Reports Second Quarter Fiscal 2009 Financial Results 7Opsona Therapeutics Closes EUR18M Funding 2Opsona Therapeutics Closes EUR18M Funding 3Opsona Therapeutics Closes EUR18M Funding 4Engineers tune a nanoscale grating structure to trap and release a variety of light waves 2Engineers tune a nanoscale grating structure to trap and release a variety of light waves 3
(Date:7/24/2014)... in German . ... they are a common sight on the roads of the ... the list of new vehicle registrations for the second time. ... where electric vehicles claim only a small portion of the ... Germany, only a mere 8000 are electric powered. The main ...
(Date:7/24/2014)... inside your gut that has gone undetected by scientists ... San Diego State University has found that more than ... described virus, named crAssphage, which infects one of the ... This phylum of bacteria is thought to be connected ... appears today in Nature Communications ., Robert A. ...
(Date:7/23/2014)... Protection Agency (EPA) today announced that environmental health ... Amherst School of Public Health and Health Sciences ... Achieve Results (STAR) grant to measure indoor air ... in subarctic North America to determine possible health ... culturally-relevant recommendations for mitigation. , Peltier, who will ...
Breaking Biology News(10 mins):Nano-supercapacitors for electric cars 2Nano-supercapacitors for electric cars 3Newly discovered gut virus lives in half the world's population 2Newly discovered gut virus lives in half the world's population 3Studying impacts of indoor air pollution on tribal communities 2Studying impacts of indoor air pollution on tribal communities 3
... the logic of inheritance in his monastery garden with no ... House. So why couldn,t Darwin have done it too? A ... influences and research focus gave him a viewpoint that prevented ... even in his own work. Darwin,s commitment to ...
... just off the east coast of Australia, picturesque North ... some of the inhabitants of the island,s creeks and ... territorial creatures, explains ecologist Robbie Wilson of the University ... one another, they size each other up in a ...
... HAVEN, FLFresh strawberries. Just the mention of this iconic ... of shortcake, fruity drinks and sweet desserts. Researchers interested ... that "sensory quality" of strawberries, a strong influence on ... of sweetness, aroma, texture, and appearance. The goals ...
Cached Biology News:Why didn't Darwin discover Mendel's laws? 2Crafty Australian crayfish cheat 2Analysis of fresh strawberries reveals consumer preferences 2
Glutamate Receptor 5/1a...
... [EP282Y] to Asparagine synthetase ( ... applications). Antigen: ... residues in the C-terminus of ... Entrez GeneID: 440 ...
Shh Antibody Shipping Temperature: HOT Storage Temperature: -20C...
Anti-human C5/C5b, Clone 568, Monoclonal Antibody...
Biology Products: