Navigation Links
Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.

p>Competition Binding Assays
The affinity of unlabeled motilin for the motilin receptor was then determined in competition assays. A competition assay of [125I]-motilin binding by motilin on motilin ScreenReady Targets showed that a monophasic competition curve was obtained with a Ki value of 5.4 nM (Figure 3). This value was in good agreement with reported values (Thielemans et al ., 2001). Taken together, results from saturation and competition experiments clearly show that genuine pharmacological properties (Kd, Ki) can be obtained for motilin ScreenReady Targets in microformatted FlashPlate assays.

Intra-plate Variability
Intra-plate variability of [125I]-motilin binding using motilin ScreenReady Targets was assessed. Membranes from CHO cells expressing the human motilin MTL-R1A receptor were immobilized into 96-well FlashPlate microplates and binding assays were performed using 48 wells for total binding determination and 48 wells for non-specific binding determination (Figure 4). The CV and S/B values led to a calculated Z factor (Zhang et al ., 1999) of 0.75, showing the robustness of the assay.

Conclusion

Using PerkinElmers proprietary coating procedure, we have developed ScreenReady Targets for homogeneous radioligand binding assays. The results obtained using the human motilin MTL-R1A receptor show that genuine pharmacological properties (Kd, Ki) can be determined and correspond to reported values (Feighner et al ., 1999; Thielemans et al ., 2001). Pharmacological analysis of the coated receptors showed that they maintain their binding properties, even after prolonged storage (3 months) at 4C. Low intra-plate variability (3.5%) on [125I]-motilin binding and a robust Z factor (0.75) demonstrate the usefulness of
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis
2. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
3. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
6. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
7. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
8. Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System
9. Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
10. Purification of the Components of Human Macular Carotenoid-Binding Complex, Rev A
11. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
Post Your Comments:
(Date:3/3/2015)...  Johnson & Johnson Innovation LLC today announced the ... (JLABS @SSF), a 30,000-square foot incubator located at ... Calif. The Johnson & Johnson Innovation, JLABS ... across the healthcare spectrum including biotech, pharmaceutical, medical device, ... resident startups have been selected, including the winners of ...
(Date:3/3/2015)... , March 3, 2015 DNA Diagnostics Center ... in the world, celebrates its 20 th anniversary ... , DDC is a consumer services company that focuses ... employs over 200 associates and generates a significant portion ... over 120 countries. Peter Vitulli ...
(Date:3/3/2015)... MINNEAPOLIS, MN , March 3, 2015 /PRNewswire/ - ... biotechnology company producing sustainable chemicals, today announced it has ... acid plant located in Sarnia, Ontario ... expected to take approximately five months, with the facility ... expects construction to be completed in two months and ...
(Date:3/3/2015)... GlobalStemCellsGroup.com has recruited Mario Camacho Alcocer ... researcher and founding partner of Criogenix SA de CV ... professors who will teach the “Diplomat in Cell Therapy ... is a private company registered in the National Record ... (RENIECYT). , Camacho Alcocer will teach two-day, interactive training ...
Breaking Biology Technology:Johnson & Johnson Innovation Opens JLABS @South San Francisco and Announces First Five Resident Startups 2Johnson & Johnson Innovation Opens JLABS @South San Francisco and Announces First Five Resident Startups 3Johnson & Johnson Innovation Opens JLABS @South San Francisco and Announces First Five Resident Startups 4Johnson & Johnson Innovation Opens JLABS @South San Francisco and Announces First Five Resident Startups 5DNA Diagnostics Center, One of The World's Largest DNA Testing Labs, Celebrates Their 20th Anniversary 2DNA Diagnostics Center, One of The World's Largest DNA Testing Labs, Celebrates Their 20th Anniversary 3BioAmber Begins Commissioning of Sarnia Commercial Plant 2BioAmber Begins Commissioning of Sarnia Commercial Plant 3Mario Camacho Alcocer Joins Global Stem Cells Group Faculty 2Mario Camacho Alcocer Joins Global Stem Cells Group Faculty 3
... 3-V Biosciences, Inc. announced today the appointment of ... (Logo:   http://photos.prnewswire.com/prnh/20100513/LA04336LOGO ) "We are ... of the 3-V senior management team," said Merdad V. ... experience in drug discovery and development, as well as ...
... fifteen minutes to analyze a sample suspected of contamination ... technology amplifies any anthrax DNA present in the sample ... cells of the deadly bacteria Bacillus anthracis. B. ... microbe that might be used intentionally to infect victims ...
... long associated with diarrhea and intestinal function may provide ... Hyperactivity Disorder) other neuropsychiatric disorders, according to a team ... Aug. 11 in Science . ... intestinal membrane receptor protein, guanylyl cyclase-C (GC-C) for its ...
Cached Biology Technology:3-V Biosciences Appoints George W. Kemble, PhD, as Chief Scientific Officer 2Nano detector for deadly anthrax 2Intestinal protein may have role in ADHD, other neurological disorders 2
(Date:2/5/2015)... , Jan. 30, 2015  It is gratifying ... power and potential for genomic science as a means ... disease prevention and treatment.  I was honored to participate ... government-funded precision medicine program. Since the ... science of genomics—from the first sequenced genome of a ...
(Date:1/22/2015)... Jan. 13, 2015 /PRNewswire/ - Today, FindBiometrics, the leading source of ... of its new website design. "When we launched ... says Peter O,Neill , founder and CEO of FindBiometrics. ... needs involvement from the key players on a very broad ...
(Date:1/22/2015)... man-made form of insulin delivered by nasal spray may ... with mild cognitive impairment and Alzheimer,s disease dementia, according ... Forest Baptist Medical Center. The study,s subjects ... (MCI) or mild to moderate Alzheimer,s dementia (AD). Those ...
Breaking Biology News(10 mins):J. Craig Venter, Ph.D., Co-Founder and CEO, Human Longevity, Inc. (HLI) Participates in White House Precision Medicine Event 2Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3
... an electromagnetic marker that predicts a patient,s response ... "Such biomarkers that identify who will benefit from ... trial-and-error prescribing and speed delivery of care for ... Zarate, M.D., of the National Institute of Mental ...
... cells facing potentially lethal antiestrogen therapy recycle to survive, ... cells have receptors for the hormone estrogen, which acts ... typically get an antiestrogen such as tamoxifen for five ... Dr. Patricia V. Schoenlein, cancer researcher in the Medical ...
... 1 OCTOBER 2008 Not only are more than ... Brown Convention Center, beginning this weekend, to participate in ... but they are bringing focus to issues of local ... theme of "Celebrating the International Year of Planet Earth," ...
Cached Biology News:Millisecond brain signals predict response to fast-acting antidepressant 2Breast cancer cells recycle to escape death by hormonal therapy 2Breast cancer cells recycle to escape death by hormonal therapy 3Earth scientists keep an eye on Texas 2Earth scientists keep an eye on Texas 3Earth scientists keep an eye on Texas 4Earth scientists keep an eye on Texas 5
... (ARCA) is a modified cap analog in ... m7G) is replaced with OCH3. Because of ... initiate transcription with the remaining hydroxyl group ... orientation. As a result, unlike transcripts synthesized ...
... structure called a ?cap? is present ... eukaryotic mRNAs and many viral RNAs. ... portion of the GTP present in ... in the synthesis of transcripts with ...
... A modified nucleotide structure called a ... of most naturally-occurring eukaryotic mRNAs and ... Analog for a portion of the ... transcription reaction results in the synthesis ...
... modified nucleotide structure called a ?cap? ... most naturally-occurring eukaryotic mRNAs and many ... for a portion of the GTP ... reaction results in the synthesis of ...
Biology Products: