Navigation Links
Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B

ted from the excited fluorophore onto the photon detector.

Noise
In all systems there are two noise components to consider, dark current noise and Shot noise. Additionally, sub-optimally designed systems may introduce noise in many different areas including the analog-to-digital conversion process or the amplification of the signal.

Dark Current noise: Even in the absence of light input, the thermal emissions from any photon-detecting device can produce a low amount of noise (measured in electrons per second) that has been termed Dark Current noise. In the case of PMTs, Dark Current noise can originate from the photocathode and/or from current leaking through the dynodes of the PMT. The GenePix 4000B has been designed to reduce dark current noise to negligible levels through two approaches: 1) the PMTs chosen for use in the GenePix 4000B show very low dark current levels, and 2) the dwell time of the lasers on each pixel is very short resulting in a negligible number of dark current electrons generated for each pixel.

Shot noise: Shot or statistical noise is the only significant type of noise that needs to be considered in the GenePix 4000B. It is generated by the input of light and results from the variable nature of photons. With shot noise, it is important to realize that as the signal intensity increases the shot noise increases as the square root of the signal collected, so the SNR actually decreases as signal intensity increases.

Signal-to-Noise ratio
Signal-to-noise ratio is used in many signal-detection disciplines (radio, electronics, imaging, etc.) as a quantitative measure of
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
2. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
3. Select the Optimal Enzyme for RT-PCR
4. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
Post Your Comments:
(Date:5/21/2015)... Patent Offering, The patent’s technology pertains to an ... within a body lumen (open space). Known as ... cost, single-use disposable illumination and camera module (housing). ... and the patent approval was received on January 6, ... the lighting and magnification of the endoscope for different ...
(Date:5/21/2015)... 21, 2015 Veolia’s environmental monitoring ... North American distribution agreement with VWR to distribute ... With more than 160 years of experience, VWR, ... for laboratory and production facilities, has cultivated a ... differentiated services to enable science. Endetec’s TECTA™ ...
(Date:5/20/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/qfxkcq/global_cell ... Cell Therapy Market Outlook 2020 " report to ... tissue and regenerative medicine is expected to have huge ... could help in the growth of deregulated organs. Several ... are unable to form new tissue or sometimes organ ...
(Date:5/20/2015)...  Marc Tessier-Lavigne, president of The Rockefeller University, ... from The Marie-Josée and Henry R. Kravis Foundation ... will be the centerpiece of the University,s major ... Kravis Research Building, two stories high, will be ... city blocks following the shoreline of the East ...
Breaking Biology Technology:IpAuctions™ Presents The Worlds Smallest Disposable Illuminated Endoscope For Auction 2IpAuctions™ Presents The Worlds Smallest Disposable Illuminated Endoscope For Auction 3Veolia’s TECTA™ B16 Instrument for E. Coli Detection is Offered Exclusively Through VWR 2Global Cell Therapy Market Outlook 2015-2020 - 262 Cell Therapies & 13 Marketed Cell Therapies 2Global Cell Therapy Market Outlook 2015-2020 - 262 Cell Therapies & 13 Marketed Cell Therapies 3The Marie-Josee and Henry R. Kravis Foundation Provides Landmark Gift of $100 Million to The Rockefeller University 2The Marie-Josee and Henry R. Kravis Foundation Provides Landmark Gift of $100 Million to The Rockefeller University 3The Marie-Josee and Henry R. Kravis Foundation Provides Landmark Gift of $100 Million to The Rockefeller University 4
... Inc., a privately held molecular diagnostics company developing innovative ... announced today the appointment of Peter Blume-Jensen, M.D., Ph.D. ... Metamark with over 20 years of experience in cancer ... Dr. Blume-Jensen was department head at EMD Serono, Inc. ...
... 16 Cord Blood America, Inc . ( ... the umbilical cord blood stem cell preservation company ... stem cells, a biological insurance policy, to families nationwide ... and CEO, was interviewed by analyst Francis Gaskins on ...
... LOUIS, Sept. 15 Sigma-Aldrich Corporation (Nasdaq: SIAL ... Healthcare Conference on Tuesday, September 21st at 12:30 PM ET ... audio broadcast over the Internet available at http://investor.sigmaaldrich.com/ . ... About Sigma-Aldrich: Sigma-Aldrich is ...
Cached Biology Technology:Metamark Genetics, Inc. Announces New Chief Scientific Officer 2Cord Blood America CEO Matthew Schissler Discusses Projects in China, South America, 'Afford-A-Cord,' and Profitability 2
(Date:5/21/2015)... 2015 According to a ... (Hardware, Software, Services), by Applications (Surveillance (Airborne, Maritime, ... End-Users (Military & Defense & Commercial) - Global ... Market is expected to grow from $7252.0 Million ... a Compound Annual Growth Rate (CAGR) of 6.6%. ...
(Date:5/19/2015)... Sweden , May 18, 2015 ... fingerprint sensors FPC1020, FPC1025, FPC1145, FPC1155 and FPC1035 from one ... from June until and including Q3 2015 and the sensors ... . Communicated order values for 2015 hereby amount to ... for Q1 2015 of 140 MSEK and a number of ...
(Date:5/14/2015)... Research and Markets ( http://www.researchandmarkets.com/research/5whpxg/fingerprint ) ... Market in Smart Mobile Devices " report to ... was a watershed year for fingerprint sensors in smartphones ... Apple gave fingerprint sensors a raison d,etre in the ... are a must-have feature in flagship smartphones. ...
Breaking Biology News(10 mins):Thermal Imaging Market Worth $9,998.9 Million by 2020 2Thermal Imaging Market Worth $9,998.9 Million by 2020 3Thermal Imaging Market Worth $9,998.9 Million by 2020 4Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Global Smart Mobile Devices Fingerprint Sensors Market 2015-2019: Key Vendors are Authentec, Validity Sensors, Fingerprint Cards, Next Biometrics Group. IDEX, and Qualcomm 2
... study by researchers at the Virginia Institute of Marine Science ... bathers and boaters, drastically altering marine food webs by shunting ... in the size and frequency of jellyfish blooms in coastal ... decades means that jellies, impact on marine food webs is ...
... part of a human cell,s control system that is able to ... as the "gating ring" can open a channel that allows a ... similar to the way a subway turnstile allows people into a ... regulation of crucial bodily activities like blood pressure, insulin secretion and ...
... Rensselaer Polytechnic Institute are combining automation techniques from oil ... closed-loop artificial pancreas. The device will automatically monitor blood ... 1 diabetes, and aims to remove much of the ... For six years, Professor B. Wayne Bequette, a member ...
Cached Biology News:Jellyfish blooms shunt food energy from fish to bacteria 2Jellyfish blooms shunt food energy from fish to bacteria 3UCLA scientists identify how major biological sensor in the body works 2UCLA scientists identify how major biological sensor in the body works 3Engineering new weapons in the fight against juvenile diabetes 2Engineering new weapons in the fight against juvenile diabetes 3
...
... fully automated solution for cell culture ... for cell-line maintenance, expansion, harvesting and ... built upon standard, industry proven components: ... configuration capable of both rapid bulk ...
... Affinity Life Sciences can provide custom microplate coating using ... washes a variety of plate formats. We can also accommodate ... volume range of 0.5-320 L):, , ... 96 well microplate, ...
... The Bio-Plex maintenance, calibration, and ... custom 96-well plate used for ... calibration procedures using the Bio-Plex ... plate is for use with ...
Biology Products: