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Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS

tly analyzed, minimizing possible derivatization problems, saving time, and preventing compound losses.

This paper describes a confirmatory research method based on HPLC and triple quadrupole mass spectrometry that is suitable for quantitative determination of CAP residues in milk and milk products in a low concentration range.

Key Features

Sensitive and selective Multiple Reaction Monitoring (MRM) scan function for linear quantitative analysis with wide dynamic range

Patented LINAC collision cell allows multi-component analysis at reduced MRM dwell times maintaining sensitivity and preventing crosstalk

Rugged and reliable triple quadrupole MS system for maximum uptime

Compatible with the broadest range of ionization sources for the analysis of a wide range of compounds

Experimental Conditions

Blank milk samples were fortified with CAP in the concentration range of 0.05 1.5 μg/L. The cleanup consisted of an acetonitrile extraction, followed by defatting with chloroform and then solid phase extraction on a styrene-divinyl benzene polymer cartridge (Table 1). The final extract was analyzed on an API 3000 LC/MS/MS system using a TurboIonSpray source in negative ion mode. Evaluation was performed by selecting the characteristic product ions for chloramphenicol and using Multiple Reaction Monitoring of four transitions m/z 321-152, 321-176, 321-194 and 321-257. For quantification, the transition m/z 321-152 was chosen. The method was validated according to the EU Commission Decision 2002/657/EC.

All solvents are HPLC-grade unless otherwise noted. Demineralized water was used in all experiments. All standard solutions of chloramphenicol (Sigma, Deisenhofen, Germany) were prepared with methanol and kept at +4C. Bond Elute LMS SPE columns of crossed linked styre
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