Navigation Links
Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS

Purpose

The European Union prohibits the use of chloramphenicol (CAP) as a veterinary drug for food producing animals. For this reason a rapid and sensitive confirmatory method for the identification and quantification of CAP in milk and milk products has been developed using the API 3000 LC/MS/MS System. Although many analytical procedures are described in the literature, the presented method has the advantages of high recovery and short analysis time. The method was validated according to the EU Commission Decision 2002/657/EC.

Overview

Chloramphenicol (Figure 1) is a broad-spectrum antibiotic previously used in veterinary medicine because of its broad range of activity and its low cost. Chloramphenicol is primarily bacteriostatic. CAP binds to the 50S subunit of the ribosome and inhibits bacterial protein synthesis. It has a wide spectrum of activity against gram-positive and gram-negative cocci and bacilli, including anaerobes.

Because of the side effects in humans, particularly dose independent fatal aplastic anemia, the use of CAP for the treatment of food-producing animals is prohibited in several countries (e.g. European Union, Canada, United States, and most Asian counties). The Commission Decision 2002/657/EC necessitates control of CAP residues in edible tissues, milk, and milk products.

Methods to detect CAP residues in biological matrices, especially in milk, include immunoassays1 and mass spectrometry in combination with GC24 and HPLC5,6. Immunological methods are suitable for screening purposes, whereas mass spectrometric methods are utilized for confirmation. Methods for the determination of CAP residues using GC/MS require additional chemistry steps, such as silylation of CAP, before the samples can be analyzed. LC/MS, on the other hand, requires no derivatization and can be direc
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Structural Determination of Flavonoids Using MSn
2. Total Cellular Protein Determination Using the DC Protein Assay
3. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
4. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
5. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
6. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
7. Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
8. Analysis Of Chloramphenicol by Negative Ion Electrospray LC/MS/MS
9. Advantages of Roche Applied Science amplification products
10. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
11. Using THERMAL DESORPTION GAS CHROMATOGRAPHY for the identification of fragrance and other components in cosmetic products
Post Your Comments:
(Date:7/3/2015)... 3, 2015 ... osoitteessa  http://www.openinnovationinscience.at Wienissä pidettävään ... Science" -jatkokoulutusohjelmaan 22.7.2015 asti.  ... tekemän, kansainvälisiä tutkijoita ja tiedemiehiä ... suurinta haastetta ovat kannustimien puute ...
(Date:7/3/2015)... 2015 Forskere kan ... in Science", som finner sted i ... nett, på  http://www.openinnovationinscience.at , og søknadsperioden varer ... De to største utfordringene innen helsevitenskapen er ... forskningsspørsmål og kompleksiteten av nåværende forskningsresultater, i ...
(Date:7/2/2015)... College of DuPage students Sam Liesman ... real-world experiences while serving biophysics internships at the prestigious ... students will be working as a part of IIT ... IIT. The internship came about from a discussion between ... Kathy Finan , Biology; and Dr. Wereszczynski regarding ...
(Date:7/2/2015)... ... July 02, 2015 , ... In’Tech Medical ... instruments in Orthopedics, announced today the acquisition of Turner Medical, Inc ., ... of surgical instruments to the Spine Industry. The acquisition of Turner Medical ideally ...
Breaking Biology Technology:Maailman ensimmäinen tiedemiesten koulutukseen suunniteltu avoimen innovaation ohjelma käynnistyy 2Maailman ensimmäinen tiedemiesten koulutukseen suunniteltu avoimen innovaation ohjelma käynnistyy 3Lansering av verdens første Open Innovation-program for utdanning av forskere 2Lansering av verdens første Open Innovation-program for utdanning av forskere 3COD Students Participate in Prestigious Summer Internships 2In'Tech Medical Acquires Turner Medical to Become a World Leader in Orthopedic Contract-Manufacturing 2In'Tech Medical Acquires Turner Medical to Become a World Leader in Orthopedic Contract-Manufacturing 3
... Nfocus device development team.PALO ALTO, Calif., April 30 ... today the appointment of accomplished regulatory executive Robert O,Holla ... President of Regulatory Affairs. "Bob O,Holla brings an unmatched ... , having served 18 of his last 30 years ...
... April 30 Idenix Pharmaceuticals, Inc. (Nasdaq: IDIX ... of drugs for the treatment of human viral diseases, today ... 31, 2009. At March 31, 2009, Idenix,s cash, cash equivalents ... , , ...
... Resonant Medical, an innovator in 3D ultrasound image-guided ... Stephen Whisenhunt to the position of Vice President ... the Company,s growing global sales team and manage ... years of radiation oncology industry experience to the ...
Cached Biology Technology:Nfocus Neuromedical Appoints Robert O'Holla to Head Regulatory 2Idenix Pharmaceuticals Reports First Quarter Financial Results 2Idenix Pharmaceuticals Reports First Quarter Financial Results 3Idenix Pharmaceuticals Reports First Quarter Financial Results 4Idenix Pharmaceuticals Reports First Quarter Financial Results 5Idenix Pharmaceuticals Reports First Quarter Financial Results 6Resonant Medical Expands Sales Organization with Appointment of Vice President of Global Sales and Marketing 2
(Date:6/17/2015)... JOSE, Calif. , June 17, 2015 /PRNewswire/ ... in human interface solutions, today announced that Xiaomi, ... adopted the Synaptics ® ClearPad ® ... of display driver integrated circuits (DDICs) for its ... Pro. By leveraging ClearPad for full in-cell display ...
(Date:6/16/2015)... June 16, 2015 Fingerprint Cards has ... sensors in the company,s portfolio from one of its module ... to mainly take place during the third quarter 2015. The ... Asia . Jörgen Lantto, CEO of FPC, ... the   growing interest from smartphone OEMs in integrating touch ...
(Date:6/15/2015)... Inc. (NASDAQ: NXTD ) ("NXT-ID" or the "Company") a ... announces that its leading payment security technology, the Wocket® smart ... NYC June 18th. Digital Experience! @ NYC is ... to over 300 of the top media covering the sector. ... New York City from 6:00 to ...
Breaking Biology News(10 mins):Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 2Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 3FPC Receives Order for Touch Fingerprint Sensors of 375 MSEK 2Wocket Smart Wallet Exhibiting at Pepcom Digital Experience! @ NYC June 18th 2Wocket Smart Wallet Exhibiting at Pepcom Digital Experience! @ NYC June 18th 3
... University of Minnesota Medical School and Masonic Cancer ... to discover how some proteins may cause the development ... part of R-spondin family normally help activate cell ... have discovered that when two types of R-spondins ...
... This press release is available in French , ... . STOCKHOLM (24 AUGUST 2012) As food ... and the "super drought" in the US, a new study finds ... insecurity and climate risks in sub-Saharan Africa and South Asia. The ...
... might have unraveled some lingering evolutionary questions about group ... that took a unique approach to observing interactions between ... in the journal Science some of the ... such as schools of fish can evolve as a ...
Cached Biology News:U of M researchers: Newly discovered genetic markers could signal colon cancer development 2Farmer-led irrigation schemes could alter food security in sub-Saharan Africa, South Asia 2Farmer-led irrigation schemes could alter food security in sub-Saharan Africa, South Asia 3Farmer-led irrigation schemes could alter food security in sub-Saharan Africa, South Asia 4Video-gaming fish play out the advantages of groups 2Video-gaming fish play out the advantages of groups 3Video-gaming fish play out the advantages of groups 4
Human peripheral blood CD14+ monocytes, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Monocytes, B Cells, NK Cells and Stem Cells...
Yeast tRNA 50 l...
...
...
Biology Products: