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Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

sensitivity necessary to support ADME studies of acepromazine in horse serum. Acepromazine was detected in horse serum for up to 2 hr after dosing whereas its metabolite was detected for up to 6 hr. The LOD achieved for acepromazine in horse serum was determined to be 0.5 ng/mL, while the LOQ for its metabolite was 0.5 ng/mL.

Acknowledgments
The authors would like to thank Lester Taylor and Diane Cho for their comments.


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