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Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

e Figures 2 and 3 show calibration curves for ACE and HEPS in horse serum with linearity over four orders of magnitude, i.e., 0.2 200 ng/mL. The R2 value is 0.9952 for ACE and 0.9922 for HEPS. These calibration curves were generated with chlorpromazine (120 ng/mL) as the internal standard and illustrate that the wide linear dynamic range of the Finnigan LCQ Deca XP Plus ion trap mass spectrometer enables quantitation of analytes in biological matrices.

Limit of Detection and Quantitation Figure 4 shows the extracted ion chromatograms for ACE and HEPS at a concentration of 0.5 ng/mL of serum. The signal to noise for ACE at 0.5 ng/mL of serum is 3:1 and hence represents the lower limit of detection (LOD) for this compound. The signal to noise for HEPS at concentration of 0.5 ng/mL of horse serum is 7:1 and represents the lower limit of quantitation (LOQ) for HEPS. Table 2 shows the lower limits of detection and quantitation for ACE and HEPS indicating that these analytes can be detected at low levels in horse serum with minimal sample preparation.

Determination of ACE and HEPS in Samples from the Horse
Figure 5 shows extracted ion chromatograms for ACE and HEPS obtained by full-scan MS/MS analysis of serum sample obtained 4-hr post administration of drug. No significant level of ACE could be detected in the horse at this time whereas its metabolite HEPS could be quantitated and its concentration determined as 1.3 ng/mL of serum. Determination of concentrations of ACE and HEPS in ng/mL of horse serum drawn at different time points post administration is tabulated in Table 3. It is seen that ACE could be detected in horse serum for up to 2 hr after dosing whereas its metabolite was detected for up to 6 hr.

Conclusions
Full-scan MS/MS analysis with a Finnigan LCQ Deca XP Plus ion trap mass spectrometer provides the selectivity and
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