Navigation Links
Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

Chromatography and Mass Spectrometry Application Report

Key Words Sensitivity Quantitation Finnigan LCQ Deca XP Plus Equine serum Acepromazine

Gargi Choudhary, Thermo Electron Corporation, San Jose, CA USA
Wayne Skinner and Scott Stanley, University of California, Davis

Introduction

Acepromazine is widely used as a sedative in horses. It serves as a tranquilizer to better facilitate handling of these large mammals before transportation or surgical procedures. Acepromazine has been classified by the Association of Racing Commissioners International, Inc. as a Class 3 drug in horses. Abuse of the drugs in the Class 3 category can result in 2-6 months of suspension, up to $1500 fine, and loss of purse. Acepromazine has a slow elimination rate and stays in the body for a long time. This can be problematic if the drug is administered before a race event since its calming effect can lead to a better performance by the horse.

Goal

Develop a method for identifying and quantitating acepromazine (ACE) and its major metabolite 2-(1-hydroxyethyl) promazine sulfoxide (HEPS) in horse serum.
Determine limits of detection (LOD) and quantitation (LOQ) of these two compounds in serum.
Demonstrate low level detection of acepromazine in a complex biological matrix.

Experimental

HPLC
HPLC system: Surveyor MS pump with Surveyor autosampler
Column: 50 2.1 mm Hypersil BDS C18 (Thermo Electron)
Injection volume: 20 μL
Flow rate: 300 μL/min
Mobile phase A: Water containing 10 mM ammonium acetate and 0.1% formic acid
B: Acetonitrile containing 0.1% formic acid
Gradient: 5-40% B in 4 min, 40-65% B in 1
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Structural Determination of Flavonoids Using MSn
2. Total Cellular Protein Determination Using the DC Protein Assay
3. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
4. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
5. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
6. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
7. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
8. Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS
9. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
10. Identification of Phase I and Phase II Metabolites of Buspirone on the Q TRAP LC/MS/MS System
11. Strategies to Identify and Confirm Phase I Metabolites of Glyburide Using the 4000 Q TRAP System
Post Your Comments:
(Date:12/24/2014)... 23, 2014 “Preparative & Process ... Flash), Consumables (Columns, Solvents, Buffers, Valves, Guages, Seals), ... Agriculture) - Forecasts to 2019” provides a detailed ... current market trends, and strategies impacting the preparative ... and forecasts of the revenue and share analysis. ...
(Date:12/24/2014)... According to Ross Selinger, President of Selinger ... the national office market continues to be strong, driven by ... by the third quarter’s surprising 3.9% GDP, unemployment decreasing to ... have held inflation down and the Fed is keeping interest ... remaining soft. , This solid economic news is having a ...
(Date:12/24/2014)... China Biologic Products, Inc. (NASDAQ: CBPO, ... integrated plasma-based biopharmaceutical company in China ... Shandong Taibang Biological Products Co. Ltd., has received ... Food and Drug Administration (the "CFDA") for its ... in the Company,s public filings, the CFDA inspected ...
(Date:12/24/2014)... YORK , Dec. 23, 2014  NeuroLifeSciences announced ... mazindol in children with attention deficit/hyperactivity disorder published in ... . The paper, titled "Pilot Phase ... disorder" ( Konofal et al, Drug Des ... ) shows that mazindol might be an effective, ...
Breaking Biology Technology:Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4ITRA Global Reports Long Island Is Out of Sync With The National Office Market 2ITRA Global Reports Long Island Is Out of Sync With The National Office Market 3China Biologic Receives GMP Certification for New Coagulation Factor Facility 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 3Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 2Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 3
... 18 HealthCore, Inc. is developing the Integrated ... practicing physicians who participate in real-world prospective effectiveness ... industry and government to understand more about how ... in real-life settings.To advance this understanding, HealthCore, the ...
... Development of Statistical Methods and SoftwareSAN DIEGO, May 18 ... Health Research & Policy at Stanford University ... Sciences. Election to the National Academy of Sciences ... accorded to a scientist in the US and this ...
... In a paper published,today in the online edition ... ) and academic colleagues from Iceland, Denmark and the,Netherlands ... sequence,of the human genome (SNPs) that influence the age ... menarche (AAM) is influenced by both genetic,and environmental factors, ...
Cached Biology Technology:HealthCore Responds to Need for Real-world Health Outcomes Information on Drugs and Devices with Physician Network and Prospective Effectiveness Research 2HealthCore Responds to Need for Real-world Health Outcomes Information on Drugs and Devices with Physician Network and Prospective Effectiveness Research 3Professor Wing Wong Elected to the National Academy of Sciences 2deCODE Study Shows Complex Interplay of the Human Biological Clock With Height, Weight and Disease 2deCODE Study Shows Complex Interplay of the Human Biological Clock With Height, Weight and Disease 3deCODE Study Shows Complex Interplay of the Human Biological Clock With Height, Weight and Disease 4
(Date:12/11/2014)... , Dec. 10, 2014  That blood pressure plays a ... while. Hypertension – the medical term for high blood pressure ... 1800s, and the inflatable cuff that,s used in measuring blood ... mean there,s nothing new about hypertension, its triggers and its ... about the condition and the best ways to treat it. ...
(Date:12/10/2014)... , Dec. 9, 2014  Valencell, a leader in ... seeing a staggering demand from its licensees for highly ... revealed, is not solely coming from fitness and health ... "A wearable is only as useful as ... is the ultimate driver in long-term mass consumer adoption ...
(Date:12/10/2014)... DUBLIN , Dec. 08, 2014 Research ... announced the addition of the "Biometrics Market ... http://photos.prnewswire.com/prnh/20130307/600769 ... for projects such as rural banking and upgradation ... major trends witnessed in the market. Besides the ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4Wearable Technology Products Demand Highly Accurate Biometric Technology 2Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2
... 2010 -- To keep soldiers in the battlefield healthy, the ... in water. Current techniques for analyzing water in ... complete, according to Bart Lipkens of Western New England College ... Andover, Ma. They are working on an alternative ...
... an artificial stomach suggest that a popular lemon-lime soft ... effectiveness of an oral anticancer drug. The experiments produced ... drug, tested in Phase I in clinical trials, when ... in ACS, Molecular Pharmaceutics , a bi-monthly journal. ...
... 10 2010) Supported by thousands of years of use, ... strong biomedical research and clinical trials, are growing in ... and prevention of a wide spectrum of diseases from cancer ... the National Cancer Institute, Wake Forest University, the University of ...
Cached Biology News:Drugstore of the future -- new drugs from an old source 2
... a convenient method to probe conformational changes in ... Thus, the kit may be used to determine ... transport substrates for MDR1. Because UIC2 binding is ... the MDR1 Shift Assay can be utilized for ...
... Zyagen tissue microarrays are derived from ... to rigorous quality control procedures to ensure ... are manually assembled in one block, sectioned ... mounted on coated slides. Sections are large ...
... derived from frozen embedded tissue blocks, which ... to ensure the highest quality. Tissues of ... block, sectioned at a thickness of 7microns ... large (3-5mm in diameter) in comparison to ...
... from Paraffin embedded tissue blocks, which are ... ensure the highest quality. Tissues of each ... sectioned at a thickness of 5 microns ... large (3-5mm in diameter) in comparison to ...
Biology Products: