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Detection of nitrofurane metabolites in food with HPLC/MS/MS

Flow: 200 μl/min, W.E.J.: during elution isocratic 50/50, AB: gradient from 95/5 to 5/95

Results

Despite their small molecular weight the derivatised metabolites can be quantitated over several orders of magnitude with high accuracy. On the API 4000 system we created for all metabolites a calibration curve from 0.5 pg to 750 pg. We determined linear regression coefficients (weighed by 1/x) of 0.999 and better. Similar coefficient were found on the API 2000 system in a concentration range of 50 pg to 5 ng.

Summary

The observed recovery was found to be 70% for SEM and AHD, 40% for AOZ and 80% for AMOZ. On the API 2000 system the quantification limit was found to be 0.5 μg/kg for AOZ and AMOZ and 1 μg/kg for SEM and AHD. The detection limits were a factor of three lower. At the API 4000 system following quantification limit were found AOZ: 0.02 μg/kg AMOZ: 0.01 μg/kg, SEM: 0.05 μg/kg and AHD: 0.1 μg/kg.

Literature


1 BgVV, Stellungnahmen vom
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