Navigation Links
Detection of nitrofurane metabolites in food with HPLC/MS/MS

Furazolidone, Furaltadone, Nitrofurazone and Nitrofurantoine belong to the group of nitrofuranes. As antibiotics they were used for treatment of gastrointestinal infections in cattle and poultry. Due to its proven mutagenic and carcinigenic effect, nitrofuranes (except for furazolidone) was prohibited in 1993 in the European Union (VO (EWG) No. 2901/93). Furazolidone was prohibited in 1995 in the European animal breeding (VO (EWG) No. 1441/95). They are mentioned in appendix IV of VO (EWG) No. 2377/90 as compunds for which no upper limits can be lay down.

Introduction

In 2002 nitrofuranes and their metabolites were frequently detected in imported poultry from Brazilia and shrimp from Asia. In this application note we present a LC/MS/MS method for detecting the nitrofurane metabolites. Since nitrofuranes have in vivo halflife times of a few hours, only their metabolites are discussed here. The metabolites are known to be very stable2: 3-Amino-2-oxazolidinone (AOZ), 3-Amino-5-morpholinomethyl- 2-oxazolidinone (AMOZ), Semicarbazide (SEM) und 1-Amino-hydantoin (AHD). The measurements shown in the following were done both on an API 2000 LC/MS/MS System and an API 4000 LC/MS/MS System. The presented method was developed on an API 2000 system in Laboratory W.E.J. The measurements on the API 4000 system were done in the Application Laboratory of Applied Biosystems (Darmstadt).

Sample Preparation

Due to their small moleculare weight it is neccessary to derivatize the metabolites with 2-nitrobenzaldehyde to achieve selective fragments. The sample treatment is shown here schematically3. AMOZ-D5 was used as an Internal Standard.

HPLC Conditions

Agilent 1100 System Column: Luna C18 (2), 150x3 mm, 3 μm, Phenomenex

Column oven: 25C

Eluent A: 0.5 mMol NH4ac B: MeOH


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Detection nitrofurane metabolites food with HPLC

(Date:11/20/2009)..., , SEATTLE, Nov. 20 /PRNewswire-FirstCall...nnounced that the U.S. Food and Drug Administratio...Company,s amended Biologics License Application (B...e response. The FDA has assigned a Prescription D...hich time it will respond to Dendreon,s amended BL...
(Date:11/20/2009)..., , STOCKHOLM, November 20 /PRNewsw...that the European Investment Fund (EIF) has entere... The co-investment fund will be,managed by Karolin...s,portfolio of early-stage pharmaceutical and medi... 26.7 M Euros in the co-investment fund. The agree...
(Date:11/20/2009)... METTLER TOLEDO recently ...ctions Process Optimization Using At-Line Particle...ember 2, 2009. In this webinar, the application o...d to map the design space and optimize a series of...s, flow rates, and roller compaction forces. , ...
(Date:11/19/2009)... , Most people would like to be able to charge th...ckly and not too often. A recent discovery made by...ube-based supercapacitors that could do just this....ysics Letters , Prabhakar Bandaru, a professor in ...gineering, along with graduate student Mark Hoefer...
Breaking Biology Technology:Dendreon Receives FDA Acknowledgement of Complete Response 2Dendreon Receives FDA Acknowledgement of Complete Response 3Karolinska Development and EIF Start Landmark Co-Investment Fund for Life Science Innovation 2Karolinska Development and EIF Start Landmark Co-Investment Fund for Life Science Innovation 3Roller Compaction Process Optimization Using At-Line Particle Characterization 2Nanotube defects equal better energy and storage systems 2
...research may have come up with an explanation as t...d rocks. This explanation could turn out to be the...production techniques with the intent of increasin...arch group at the Nano-Science Center, part of the...n has investigated drill cores collected from Nort...
..., May 11 Sanofi-aventis U.S. announced today that...ma plc the U.S. commercial rights to Oforta(TM) (f... B-cell chronic lymphocytic leukemia (CLL) drug. ... for use as a second-line therapy to treat adults ...to add Oforta to our existing oncology portfolio, ...
...May 11 Pharmasset, Inc. (Nasdaq: VRUS ), a clin...covering, developing, and commercializing novel dr...results and operational highlights for the fiscal ...ults , , Revenues were $1.9 million during the q...on for the quarter ended March 31, 2008 and reflec...
Other Biology Technology:New Danish research shows how oil gets stuck underground 2Sanofi-aventis U.S. to Acquire Oral Chronic Lymphocytic Leukemia Treatment 2Sanofi-aventis U.S. to Acquire Oral Chronic Lymphocytic Leukemia Treatment 3Pharmasset Reports Fiscal Second Quarter 2009 Financial Results 2Pharmasset Reports Fiscal Second Quarter 2009 Financial Results 3Pharmasset Reports Fiscal Second Quarter 2009 Financial Results 4Pharmasset Reports Fiscal Second Quarter 2009 Financial Results 5Pharmasset Reports Fiscal Second Quarter 2009 Financial Results 6Pharmasset Reports Fiscal Second Quarter 2009 Financial Results 7Pharmasset Reports Fiscal Second Quarter 2009 Financial Results 8
(Date:11/23/2009)...l Medical Sciences (NIGMS), part of the National I...ion for grants in scientific areas it identified a... grant program to stimulate biomedical research an... Recovery and Reinvestment Act (Recovery Act). , ... a significant impact on a field of biomedical sci...
(Date:11/23/2009)...rector of the Interuniversity Professorship in Law...e University of the Basque Country. The Professors...er experts such as researchers in the fields of mo...ethics. , The Interuniversity Professorship in L...usto and the Basque Country was created in 1993. T...
(Date:11/23/2009)...erman . , Of all global carbon dioxide emiss...where it contributes to global warming. The remain...stems such as forests, grasslands and peat-lands. ...restrial ecosystems is considered one of the main,...ange. However, new greenhouse gas bookkeeping has ...
Breaking Biology News(10 mins):NIGMS invests in scientific Grand Opportunities with Recovery Act funds 2NIGMS invests in scientific Grand Opportunities with Recovery Act funds 3Research and legislation should go hand in hand, as much as possible 2Intensive land management leaves Europe without carbon sinks 2Globus Medical Announces Launch of COALITION 28TM 29 ACDF Spacer System 47604 1Globus Medical Announces Launch of COALITION 28TM 29 ACDF Spacer System 47604 2EXTRA TV Features Tosca Renos Eat Clean Diets Libido Boosting Foods 47600 1EXTRA TV Features Tosca Renos Eat Clean Diets Libido Boosting Foods 47600 2Genomatica Produces Pure BDO From Natural Sugars 12413 1Genomatica Produces Pure BDO From Natural Sugars 12413 2Genomatica Produces Pure BDO From Natural Sugars 12413 3Genomatica Produces Pure BDO From Natural Sugars 12413 4
...ll needs both to build new and to degrade old or d...ntracellular systems work in concert to keep the c...eins. When proteins or peptides mutate, they can p...tracellular environment. In Huntington,s disease (...in gene eventually causes the cell to build up int...
...cientists has announced a major expansion of a lin...s in living organisms. The expansion provides ter... events that occur when a pathogenic or beneficial...events is crucial for developing new interventions...obes while preserving or encouraging the presence ...
...they won,t be able to understand the brain until t...how billions of neurons are interconnected. Now, r...udies have jumped what many believe to be a major ... the connections to a single neuron. , In the Marc...escribe how they modified the deadly rabies virus,...
Other Biology News:Proteasome activator enhances survival of Huntington's disease neuronal model cells 2Uniform language for describing genes of pathogenic and beneficial microbes 2Uniform language for describing genes of pathogenic and beneficial microbes 3Deconstructing brain wiring, one neuron at a time 2Deconstructing brain wiring, one neuron at a time 3
5X StabilCoat Immunoassay Stabilizer from SurModics, Inc.
...llow crystalline solid. PROTECT FROM LIGHT. Useful...response data are not required. Purity: ≥98% b...us systems, preparation of a 10 mM stock in DMSO i... can be added to H 2 O with rapid mixing (e.g. 10 ...
Mouse Anti-Fatty Acid Binding Protein (FABP) Antibody, TRITC (Rhodamine) Conjugated from HyTest Ltd.
Mouse Anti-Rhodopsin Monoclonal Antibody, Unconjugated, Clone RET-P1 from GeneTex
Biology Products: