Navigation Links
Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

different denaturant concentrations. Virtually all of the alleles were differentiated by DGGE, with the exception of two sets of alleles with a single C-G transversion within 10 bp of the clamped primer and one set of alleles with a single A-T transversion at nt 237. The remaining 5 sets of alleles with single base differences at positions ranging from nt 85 to 282 (24 to 243 not including 5'-clamped primer) were differentiated, as well as all alleles with a greater number of differences. Thus, the range of denaturant gradient used and the addition of the GC-clamp facilitated sampling of sequence variation over virtually the entire A1 fragment.

As expected from the presence of two melting domains within the A1 sequence, time series analysis revealed a number of alleles which shifted relative positions on the gel when electrophoresed into different ranges of denaturant concentration. Moreover, some alleles that migrated differentially through the gel at low concentrations of denaturant became indistinguishable when electrophoresed in high denaturant concentrations (Figure 3). As a result, there was no single run-time that consistently separated all of the alleles. In the time series shown in Figure 3, the best separation of alleles was obtained at a run-time of 5.5 hr, which placed the DNA fragments between 48% and 53% denaturant, a range in which dissociation of the A1 low melting domain was completed and the high melting domain was initiated. At this run-time, 18 alleles were distinctive, and 5 sets of alleles migrated to similar positions and were consequently binned into 5 allelic groups.

For the homoduplex A1 allele fragments, increasing mig
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:5/6/2015)... 2015 Dr Jaspreet Singh , Senior ... deliver a speech at the 2015 Asian Starch Conference, to ... Expo Center.  , ... the topic: ,Potato Starch: A Potential Source for the ... Co-editor of a 528 page text book entitled ...
(Date:5/5/2015)... , May 5, 2015 Roka Bioscience, Inc. (NASDAQ: ... advanced testing solutions for the detection of foodborne pathogens,  today ... the Bank of America Merrill Lynch 2015 Health Care Conference ... held at Encore at the Wynn, Las Vegas ... the presentation will be available through the investor relations section ...
(Date:5/5/2015)... May 5, 2015 Psyche Systems Corporation and ... offers a first-of-its-kind platform that helps labs comply with ... results, transforms the way patients engage with these results, ... Health provides a HIPAA-compliant digital platform that gives ... and understand their lab results, and uniquely connects labs, ...
(Date:5/5/2015)... ImmunoCellular Therapeutics, Ltd. ("ImmunoCellular") (NYSE MKT: ... and Chief Executive Officer, will present live at ... May 7, 2015     TIME:  11:30 am ET/8:30 am ... the red "register/ watch presentations" button ... where investors are invited to ask the company ...
Breaking Biology Technology:Potato Starch: A Potential Source for the Creation of Next Generation Bulk-ingredients and Smart Foods 2Psyche Systems Corporation Forms Strategic Alliance with Luminate Health to Provide First-of-its-kind Patient Access Solution 2Psyche Systems Corporation Forms Strategic Alliance with Luminate Health to Provide First-of-its-kind Patient Access Solution 3ImmunoCellular Therapeutics to Webcast Live at VirtualInvestorConferences.com on May 7 2
... 15 BlueFire Ethanol Fuels, Inc. (OTC Bulletin Board: ... fuel paradigm through the production of ethanol from non-food ... second planned biorefinery to Fulton, Mississippi. , (Logo: ... having been awarded a $40 million dollar grant from ...
... ... Stiris Research has selected the Clinical Force CTMS to manage clinical trials. Stiris ... Clinical Force, Stiris Research anticipates significant study cost efficiencies that it can pass on ... ...
... SACRAMENTO, Calif., Oct. 14 The inaugural Advanced Bioenergy ... the Sacramento Convention Center in Sacramento, Calif. will feature ... of the bioenergy value chain. , During the opening ... advisor at Piper Jaffray & Co., will present ...
Cached Biology Technology:Department of Energy Grants BlueFire Approval for Site Change 2Department of Energy Grants BlueFire Approval for Site Change 3Department of Energy Grants BlueFire Approval for Site Change 4Clinical Force Announces that Stiris Research has Selected the Clinical Force CTMS to Manage Clinical Trials 2Clinical Force Announces that Stiris Research has Selected the Clinical Force CTMS to Manage Clinical Trials 3Internationally Recognized Bioenergy Experts Convene in Sacramento 2
(Date:4/27/2015)... , Apr. 27, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market, ... to pre-order customers the first week of May, 2015 ... month of May. Gino Pereira ... milestone for the company as Wocket® enters the consumer ...
(Date:4/21/2015)... , April 21, 2015 ... evolving government policies are boosting access control systems market ... According to a recently published report by TechSci ... 2020 ", the access control systems market in ... USD1.2 billion by 2020.The access control systems market in ...
(Date:4/20/2015)... , April 20, 2015 The ... Glenbeigh Records Management (GRM), Ireland,s ... Dubai Islamic Bank. Having built up an impressive track record ... a leading player within the records management sector in ... to double is GCC staffbase and employ a further eight ...
Breaking Biology News(10 mins):Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 3Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3
... established in 1990 to emphasize to the general public ... play in our lives. Ullyot Lectures are held annually ... distinguished in their fields, nationally recognized, and able to ... will be given by Bernard Bigot, high commissioner of ...
... International (CI) today announced that it has received a ... support of its conservation efforts to protect the endangered ... event launch for the DVD and Blu-ray debut of ... and its Chief Executive Officer Jeffrey Katzenberg pledged $1 ...
... Tufts University have gained further understanding of the ... content of uracil in blood DNA. In a ... Clinical Nutrition , senior author Jimmy Crott, PhD, ... in five genes involved in folate uptake and ...
Cached Biology News:Conservation International gets support from Dreamworks Animation to protect giant pandas 2SNPs affect folate metabolism in study of Puerto-Rican adults 2SNPs affect folate metabolism in study of Puerto-Rican adults 3
... , Ultima II Series ... Ultima II represents the finest, most enhanced ... and blood bank configurations, each Ultima II ... system with built-in voltage boost and surge ...
... ultra low temperature freezers offer a ... storage of biological samples with high ... separate inner doors ensure excellent warming ... ,Application oriented accessories simplify tracking ...
... the ultimate tool for both microplate ... are two editions of SkanIt Software ... working in life science research, and ... needed for compliance with FDA's 21 ...
...
Biology Products: