( eafter no apparent skin disruption he...)Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

eafter, no apparent skin disruption, hematoma, or bleeding were observed. One week later, mice were scarified and abdominal skin was analyzed for the presence of -galactosidase. Several blue stained epidermal cells were detectable in murine skin bombarded with pCMV--gal-coated microcarriers as it is demonstrated in Figure 4A. No blueness occurred in murine epidermal cells bombarded with pCMV-coated microcarriers (Figure 4B) demonstrating the specificity of the expression induced by the Helios gene gun-mediated DNA transfer. Using this method we found that microcarrier-mediated gene transfer under in vivo conditions a fast, convenient, and efficient procedure to express foreign DNA using only a minimal amount of plasmids.

References
1. Klein, T. M., Arentzen, R., Lewis, P. and Fitzpatrick-McElligot, S., Transformation of microbes, plants, and animals by particle bombardment, Bio/Technol., 10, 286-291 (1992).

2. Sanford, J. C., Klein, T. M., Wolf, E. D. and Allen, N., Delivery of substances into cells and tissue using a particle bombardment process, Part. Sci. Technol., 5, 27-37 (1987).

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