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Detection of Platinum Species in Plant Material by Preparative Isotachophoresis Using the Model 491 Prep Cell, Rev A

the gel with the same velocity and in the order of their net mobilities. The net mobilities of the sample ions must be between the mobilities of the leading electrolyte and the terminating electrolyte. When the system has reached equilibrum, each zone travels in immediate contact with its neighbors and the velocity of these zones is the same as that of the leading electrolyte. The electrochemical process is similar to the more familiar stacking process, but more extended geometrically. Each zone contains only the sample component and the counter ion. In isotachophoresis, there is an efficient counteraction of diffusion as a consequence of a zone sharpening phenomenon. Sample zones maintain sharp boundaries resulting in high resolution and highly concentrated zones.

In order to resolve the isotachophoretically-separated zones it is necessary to add spacer ions, consisting of carrier ampholytes, to spatially separate sample zones.

Running Conditions
The conditions of the isotachophoretic separation technique with the Model 491 Prep Cell were as follows.

The 28 mm ID gel tube was used.
1. Separating gel (non-restrictive gel): A monomer concentration of 5% T with a crosslinker concentration of 5% C was used. A 100 ml amount of the leading electrolyte was mixed with 4.75 g of acrylamide and 0.25 g of N,N- methylenebisacrylamide (Bis). After degassing this solution, 20 l of TEMED and 200 l of 10% (w/v) ammonium persulfate solution (100 mg ammonium persulfate + 1.00 ml water) were added to initiate chemical polymerization. The separating gel was cast in the tube assembly to a height of about 7 cm following the procedures detailed in the instruction manual. The monomer solutio
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