Navigation Links
Detection of Platinum Species in Plant Material by Preparative Isotachophoresis Using the Model 491 Prep Cell, Rev A

l pollution.1 The emission of platinum as small particles (sub- m range) increases the bio-availability of this element2,3 and has created interest in biological studies of platinum. For the separation and characterization of platinum species in grass cultivations, preparative isotachophoresis with the Model 491 Prep Cell leads to excellent separations. Sharply isolated platinum- containing fractions were obtained, which were further characterized biochemically.


Methods

The combined procedure for the separation, detection, and characterization of platinum-containing species in plant materials is described in references 4 and 5.

Briefly, extracts of untreated and platinum-treated grass cultivations were separated by combined use of two different preparative methods with different separation principles: gel permeation chromatography followed by isotachophoresis in the Model 491 Prep Cell. This combined procedure leads to an excellent recovery of purified platinum binding proteins. The detection of the platinum species was carried out sequentially by absorptive voltammetric platinum determination in individual fractions. Native electrophoresis (PAGE) was used for further characterization of selected species.

Isotachophoresis
Following chromatography4,5, a second preparative separation step of isotachophoresis gave best results. In isotachophoresis, the upper buffer is called the terminating electrolyte and the lower buffer is the leading electrolyte. The non-restrictive polyacrylamide gel is polymerized containing the leading electrolyte. During electrophoresis, charged molecules electrophorese through
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:10/30/2014)... CARLSBAD, Calif. , Oct. 30, 2014 Isis ... antisense therapeutics, today announced that management will present a company ... 2014 at 8:30 a.m. ET in Boston, MA. ... will be available on the "Investors & Media" section of ... available on the Isis website within 48 hours and will ...
(Date:10/30/2014)... N.J. , Oct. 30, 2014  Regado Biosciences, ... it will hold a conference call and live audio ... EST to discuss its third quarter 2014 financial results. ... conference call by dialing (888) 347-1165 for domestic callers ... will be webcast live under the investor relations section ...
(Date:10/30/2014)... The severe flooding that devastated a wide swath of ... the bridges, roads and other infrastructure had been upgraded ... University of Colorado Denver. , "People need to understand ... PhD, associate professor of structural engineering at the CU ... author the study. "There is an assumption that a ...
(Date:10/27/2014)... October 27, 2014 The new ... Type (High & Medium), Sub-type (Primary & Secondary), ... Infrastructure & Transportation & Power Generation) - Trends ... GIS market with analysis and forecasting of the ... Data Tables with 52 Figures spread through 146 ...
Breaking Biology Technology:Isis Pharmaceuticals to Present at the Nomura Biotechnology Conference 2Regado Biosciences to Provide Third Quarter 2014 Financial Results 2CU Denver study says upgrading infrastructure could reduce flood damage 2Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 2Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 3Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 4
... BioTechnologies, Inc. (TriLink) and Lucigen Corporation (Lucigen) announced today ... for the use of TriLink,s CleanAmp™ dNTPs in Lucigen,s ... CleanAmp™ dNTPs are an innovative approach to Hot Start ... PCR assay with CleanAmp™ dNTPs, a Hot Start reaction ...
... DIEGO, July 28, 2011 Verenium Corporation (NASDAQ: ... commercialization of high-performance industrial enzyme solutions, today announced that ... its 5.5% Notes and approximately $3 million in principal ... very pleased to have taken another important step toward ...
... July 28, 2011 United Therapeutics Corporation (NASDAQ: ... ended June 30, 2011. "I,m pleased with the ... as the very good operating results we achieved," remarked Martine ... remain on track to reach our 2011 forecast for revenues ...
Cached Biology Technology:TriLink BioTechnologies, Inc. Announces License Agreement with Lucigen Corporation 2Verenium Announces Debt Repurchase 2Verenium Announces Debt Repurchase 3United Therapeutics Corporation Reports Second Quarter 2011 Financial Results 2United Therapeutics Corporation Reports Second Quarter 2011 Financial Results 3United Therapeutics Corporation Reports Second Quarter 2011 Financial Results 4United Therapeutics Corporation Reports Second Quarter 2011 Financial Results 5United Therapeutics Corporation Reports Second Quarter 2011 Financial Results 6United Therapeutics Corporation Reports Second Quarter 2011 Financial Results 7
(Date:10/28/2014)... National University of Singapore (NUS) have discovered a new ... laying direct developing eggs in live bamboo with narrow ... bush frog ( Raorchestes chalazodes ). This critically ... known to adopt this novel reproductive strategy. The findings ... Biological Journal of the Linnean Society , in October ...
(Date:10/28/2014)... juices are associated with a lower risk of developing ... of East Anglia (UEA). , Research published today ... flavanones (both subclasses of dietary flavonoids) significantly decrease their ... of cancer death among women. , The research ... between 25 and 55 for more than three decades. ...
(Date:10/27/2014)... German . ... via electrical signals, they communicate with each other at ... substances, the neurotransmitters, are stored in vesicles at the ... vesicles fuse with the cell membrane and release their ... synapses always have some readily releasable vesicles on standby. ...
Breaking Biology News(10 mins):NUS researchers discover for the first time that a rare bush frog breeds in bamboo 2NUS researchers discover for the first time that a rare bush frog breeds in bamboo 3Tea and citrus products could lower ovarian cancer risk, new UEA research finds 2Synapses always on the starting blocks 2Synapses always on the starting blocks 3
... they know to fear the scent of a predator. But how ... cat is nearby? It,s a complex process that starts ... noses. But until now it wasn,t clear exactly how these scent ... a study to be published in Nature (available online ...
... is relatively common to come across petrol stations surrounded by ... Murcia (UM) have studied the effects of contamination at petrol ... noted in buildings less than 100 metres from the service ... which increases the risk of cancer have been recorded ...
... It,s the champagne of the cheese world and the ... like Stilton are literally under the microscope in a quest ... of Nottingham and The University of Northampton are working with ... distinctive taste, texture and smell. The scientists hope to ...
Cached Biology News:The brain knows what the nose smells, but how? Stanford researchers trace the answer 2The brain knows what the nose smells, but how? Stanford researchers trace the answer 3Petrol stations pollute their immediate surroundings 2The quest for the ultimate blue cheese 2
... Power Supply, 1. 300 V, 400 ... constant current modes.Single-unit increments in settings ... for submarine, mini vertical, and standard ... semidry and mini tank blotting applications. ...
B3-1...
Human CCL26/Eotaxin-3 MAb (Clone 115002)...
Normal sheep serum collected from healthy normal sheep...
Biology Products: