Navigation Links
Detection of Platinum Species in Plant Material by Preparative Isotachophoresis Using the Model 491 Prep Cell, Rev A

l pollution.1 The emission of platinum as small particles (sub- m range) increases the bio-availability of this element2,3 and has created interest in biological studies of platinum. For the separation and characterization of platinum species in grass cultivations, preparative isotachophoresis with the Model 491 Prep Cell leads to excellent separations. Sharply isolated platinum- containing fractions were obtained, which were further characterized biochemically.


Methods

The combined procedure for the separation, detection, and characterization of platinum-containing species in plant materials is described in references 4 and 5.

Briefly, extracts of untreated and platinum-treated grass cultivations were separated by combined use of two different preparative methods with different separation principles: gel permeation chromatography followed by isotachophoresis in the Model 491 Prep Cell. This combined procedure leads to an excellent recovery of purified platinum binding proteins. The detection of the platinum species was carried out sequentially by absorptive voltammetric platinum determination in individual fractions. Native electrophoresis (PAGE) was used for further characterization of selected species.

Isotachophoresis
Following chromatography4,5, a second preparative separation step of isotachophoresis gave best results. In isotachophoresis, the upper buffer is called the terminating electrolyte and the lower buffer is the leading electrolyte. The non-restrictive polyacrylamide gel is polymerized containing the leading electrolyte. During electrophoresis, charged molecules electrophorese through
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:7/10/2014)... July 10, 2014 EvoDerma ’s NOOME ... This rejuvenating device now comes with a second treatment cup ... time. , The new cup is thinner on the edges ... to target rough, thin and uneven surfaces on the face, ... for a stimulating treatment on areas such as the cheeks ...
(Date:7/10/2014)... , July 10, 2014  Franciscan St. ... of capnography for respiratory monitoring outside the ... healthcare leaders in embracing state-of-the-art patient safety ... patients are breathing and can alert medical ... measuring the amount of carbon dioxide the ...
(Date:7/10/2014)... , July 10, 2014  Kainos Capital, a ... brands, today announced that it has acquired the Slim-Fast ... in the business. Terms of the transaction were not ... and meal replacement business that markets ready-to-drink shakes, powders, ... North America and in the ...
(Date:7/10/2014)... -- Research and Markets ( http://www.researchandmarkets.com/research/s3bqj2/photonic ) has ... Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... traces its roots in the latter half of ... went unexplored and unfulfilled for several decades owing ...
Breaking Biology Technology:Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2Kainos Capital Acquires Slim-Fast From Unilever 2International Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) Market - Forecasts to 2019 2
... , ... Peregrine Pharmaceuticals, Inc. (Nasdaq: PPHM ) and ... entered into a licensing agreement for antibody therapeutic rights under ... Under the terms of the agreement, Affitech will license exclusive ...
... , ROCKVILLE, Md., July 22 ... selection of a Respiratory Syncytial Virus (RSV) vaccine candidate that ... Investigational New Drug (IND) application. As previously announced, Novavax ... of which have successfully induced antibody responses in mice. ...
... , , , ... Biotech, Inc., a company personalizing spine care through the development ... that they have entered into a collaboration that allows Axial ... Population Database (UPDB). UPDB is a genealogical and family history ...
Cached Biology Technology:Peregrine Pharmaceuticals Licenses Anti-VEGF Antibodies to Affitech 2Peregrine Pharmaceuticals Licenses Anti-VEGF Antibodies to Affitech 3Peregrine Pharmaceuticals Licenses Anti-VEGF Antibodies to Affitech 4Peregrine Pharmaceuticals Licenses Anti-VEGF Antibodies to Affitech 5Novavax Announces Selection of a Respiratory Syncytial Virus Vaccine Candidate for Advanced Preclinical Studies 2Novavax Announces Selection of a Respiratory Syncytial Virus Vaccine Candidate for Advanced Preclinical Studies 3Novavax Announces Selection of a Respiratory Syncytial Virus Vaccine Candidate for Advanced Preclinical Studies 4Axial Biotech Enters Partnership with the University of Utah to Utilize the Utah Population Database to Develop Personalized Diagnostic Tests for Spine Disorders 2Axial Biotech Enters Partnership with the University of Utah to Utilize the Utah Population Database to Develop Personalized Diagnostic Tests for Spine Disorders 3
(Date:7/10/2014)... Research and Markets ( http://www.researchandmarkets.com/research/h7h8lq/gesture ) ... Gesture Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, ... report to their offering ... Touch-Less Sensing Market to Grow Exponentially. Touch-less sensing ... the companies were unable to leverage this technology ...
(Date:7/10/2014)... 2014 Fingerprint Cards, (FPC,s) ... win (DW). An Asian OEM has selected FPC1021 for a ... in August 2014.   FPC is proud to ... a planned date for start of mass production in August ... SEK 5M, for delivery in the first half of July. ...
(Date:7/10/2014)... belongs to the large, mainly tropical flowering plant family Melastomataceae ... the family rely on bees for their pollination. Only about ... vertebrates. The flowers of Axinaea appear in clusters ... of the different species are pink, yellow, orange or red. ... contrasting colours of their bulbous appendages. The pollination mechanism of ...
Breaking Biology News(10 mins):Global Gesture Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, Capacitive) Market - Forecasts to 2020 2FPC Wins DW and Initial Ramp Order for FPC1021 Touch Sensor for Flagship Smartphone from Asian OEM 2Novel type of bird pollination mechanism discovered in South America 2
... ... Presidential Early Career Award for Scientists and Engineers (PECASE). The award ... scientists and engineers in the early stages of their careers. ... significant award," said Jane Lubchenco, Ph.D., under secretary of commerce for ...
... Researchers at the University of Missouri have identified a ... may provide new insight into the debilitating and fatal ... "Critical communication occurs at the point where nerves and ... nerves and muscles is disrupted, muscles do not work ...
... -- The National Institutes of Health (NIH) has awarded a ... research into risk factors for spina bifida and related congenital ... or brain is not fully enclosed. The research will ... E. Mason at Weill Cornell Medical College. Dr. Ross is ...
Cached Biology News:President to honor high achieving, early career NOAA scientists 2MU researchers find new insight into fatal spinal disease 2NIH awards $5.5 million grant to Weill Cornell for research into preventing spina bifida 2NIH awards $5.5 million grant to Weill Cornell for research into preventing spina bifida 3