Navigation Links
Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A

om 12 g/well to 3 g/well (Figure 2A), which suggests that further dilution is necessary to detect a dose response. The Bio-Plex assay showed a distinct dose response (Figure 2B) that correlated well with the western blot (Figure 2C).

Total ERK
Total ERK1/2 was detected by ELISA in HEK 293 lysates with protein concentration ranging from 10 g/well to 0.625 g/well and absorbance ranging from 0.331 to 0.160 (Figure 3A). To explore the Bio-Plex total ERK2 assay sensitivity further, lysates were further diluted to a protein concentration range of 1.250.078 g/well (Figure 3B). In comparison, a faint band was visible on the western blot at a total protein concentration of 0.625 g/lane (Figure 3C).

Both sandwich antibody methods detected total ERK in HeLa lysates with protein concentration ranging from 13 g/well to 0.41 g/well. A flattened lower-end dose response was observed with the ELISA, and an absorbance range of 0.5000.158 showed a limited ability to distinguish between different samples (Figure 3D). The Bio-Plex total ERK2 assay better distinguished signal intensities from the high end to the low end, with MFI from 11,713 to 603 (Figure 3E). The western blot band became faint at 3.25 g/well, indicating the level of sensitivity compared to the Bio-Plex assay (Figure 3F).

Phospho-ERK
Epidermal growth factor (EGF)-treated HEK 293 cell lysates were used for phospho-ERK (Thr202/Tyr204) assay comparison. Lysates were prepared using the Bio-Plex cell lysis kit or by following a protocol provided with the ELISA kit. ELISA showed a limited ability to distinguish the first two samples, with total protein concentrations of 2 g/
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:8/22/2014)... published today (August 22nd), in the scientific journal ... Neuroscience Programme establish the effect of serotonin on ... genetic and optical techniques., "Serotonin is a small ... range of brain functions, from the control of ... emotional behaviours, This neurotransmitter is also popularly thought ...
(Date:8/22/2014)... 22, 2014 Research and Markets ... China Gel Permeation Chromatography (GPC) Industry Report 2014" ... and China Gel Permeation Chromatography (GPC) Industry Report is ... of the global gel permeation chromatography industry with a ... a basic overview of the industry including definitions, classifications, ...
(Date:8/22/2014)... 22, 2014 “Our nation's healthcare ... a national disgrace. Furthermore, every governmental and professional ... current, deplorable state of unorganized dysfunction shares in ... to merely serve as a device to use ... colossal failure(s) that have accumulated through the years ...
(Date:8/21/2014)... Dallas, Texas (PRWEB) August 21, 2014 ... Global Seaweed Fertilizer Industry” is a professional ... Fertilizer market. The report provides basic Seaweed ... application, and industry chain structure as well ... international market analysis, including domestic market as ...
Breaking Biology Technology:From happiness to pain: Understanding serotonin's function 2Global and China Gel Permeation Chromatography (GPC) Industry Report 2014 25 Great Mistakes in Healthcare and How to Fix Them by Dr. Ira Williams 2Internationals Seaweed Fertilizer Industry 2014 Development Trend Analysis in New Research Study at DeepResearchReports.com 2Internationals Seaweed Fertilizer Industry 2014 Development Trend Analysis in New Research Study at DeepResearchReports.com 3
... Prep for qRT-PCR , ... (qRT-PCR) is the most sensitive way to detect and quantitate , ... validate techniques that monitor , ... interference experiments. The Cells-to-Signal Kit (patent , ...
... , ... cells grown in suspension, and other difficult-to-transfect , ... range of conditions , ... cells quickly with included positive and negative controls , ...
... The p Silencer ... enables researchers to study the long-term effects of reducing , ... retroviral vectors make use of H1 or U6 ... to stably express siRNAs, even in , ...
Cached Biology Technology:Fast, Accurate Assessment of siRNA-induced Gene Silencing 2Fast, Accurate Assessment of siRNA-induced Gene Silencing 3Fast, Accurate Assessment of siRNA-induced Gene Silencing 4Fast, Accurate Assessment of siRNA-induced Gene Silencing 5Deliver siRNAs Into Primary Cells 2Deliver siRNAs Into Primary Cells 3Deliver siRNAs Into Primary Cells 4Deliver siRNAs Into Primary Cells 5Deliver siRNAs Into Primary Cells 6Enhanced siRNA Delivery and Long-term Gene Silencing 2Enhanced siRNA Delivery and Long-term Gene Silencing 3Enhanced siRNA Delivery and Long-term Gene Silencing 4Enhanced siRNA Delivery and Long-term Gene Silencing 5Enhanced siRNA Delivery and Long-term Gene Silencing 6Enhanced siRNA Delivery and Long-term Gene Silencing 7Enhanced siRNA Delivery and Long-term Gene Silencing 8Enhanced siRNA Delivery and Long-term Gene Silencing 9Enhanced siRNA Delivery and Long-term Gene Silencing 10
(Date:8/21/2014)... geologist Juliane Mller the Fram Strait is a key region ... this passage between Greenland and Svalbard warm Atlantic water flows ... west side cold Arctic water masses and sea ice push ... A considerable portion of the Atlantic water cools here on ... The circulation of the water caused in this manner drives ...
(Date:8/21/2014)... A study recently published in the journal Carcinogenesis ... a new role for the protein adenomatous polyposis coli ... of cancer-related deaths in the U.S. , Lead author ... Biosciences and co-leader of the Cancer Biology program at ... of her career trying to understand the various activities ...
(Date:8/21/2014)... Australian National University (ANU) team has successfully replicated one ... for biological systems powered by sunlight which could manufacture ... so is sunlight. It is an exciting prospect to ... and safely," said Dr Kastoori Hingorani, from the ARC ... Research School of Biology. , Hydrogen offers potential as ...
Breaking Biology News(10 mins):Arctic sea ice influenced force of the Gulf Stream 2Arctic sea ice influenced force of the Gulf Stream 3Research offers insight into cellular biology of colorectal cancer 2Research offers insight into cellular biology of colorectal cancer 3Water and sunlight the formula for sustainable fuel 2
... Society,s (ACS,) award-winning Global Challenges/Chemistry Solutions podcast ... without access to flush toilets or other adequate sanitation ... a report by Jamie Bartram, Ph.D., and colleagues in ... new podcast is available without charge at iTunes and ...
... cold water on the idea that planting trees can ... Brendan Mackey of Griffith University Climate Change Response Program ... researchers from Australia and the U.K. Their findings are ... and climate change mitigation policy", published in the scientific ...
... Forty-two may or may not be the answer to everything, ... thrives, according to the first laboratory study of heat-loving Pompeii ... access journal PLOS ONE by Bruce Shillito and ... The worms, named Alvinella pompejana , colonize black smoker ...
Cached Biology News:Forest and soil carbon is important but does not offset fossil fuel emissions 2
Rabbit polyclonal to NMDAR1 e/f/g/h ( Abpromise for all tested applications)....
Purified anti-PI3K p110 delta...
Peptide-affinity Purified Polyclonal Antibody to Histamine H2 Receptor...
Mouse Wnt-9b Biotinylated Affinity Purified PAb ENTREZ GeneID: 22412...
Biology Products: