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Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

on of restriction digest and DGGE analysis, we have developed a technique that can be used to distinguish between previously characterized non-coding changes in the Fas antigen, and unknown mutations. Identification of the known polymorphism reduces the number of bands which must be fully sequenced, and contributes to the overall efficiency of the genetic screening.


References

1. Eng, C. and Vijg, J. Nature Biotech., 15, 422426 (1997).

2. Landowski, T. H., Qu, N., Buyuksal, I., Painter, J. S. and Dalton, W. S., Blood (1997).

3. Sheffield, V. C., Cox, D. R., Lerman, L. S. and Myers, R. M. Proc. Natl. Acad. Sci. USA, 86, 232236 (1989).

4. Fiucci, G. and Ruberti, G. Immunogen., 39, 437439 (1995).

5. Myers, R. M., Maniatis, T. and Lerman, L. S., Meth. Enz., 155, 501527 (1987).


The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffmann-LaRoche. Use of the PCR process requires a license.


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