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Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS

oid caused (usually) by a high concentration of salt eluting from the LC column.

Conclusion

This application note demonstrates the picogram detection, without derivatization, of morphine and its metabolite morphine-glucuronide in saliva or urine. The limit of quantitation for these analytes is well within the limits set by NIDA and SAMHSA for the detection of opiates in saliva and/or urine. More importantly, this method does not require the lengthy and often tedious derivatization as required in a GC/MS method. All compounds were found to be linear in the calibration range tested and all had very good correlation coefficients (average r2= 0.989).

Finally, this application demonstrates the use of two useful features of the 500-MS, the use of the built-in 6-port injection valve and the syringe pump. Together they can be used as a diverter valve to avoid potential damage to the electrospray interface by diverting salts away from the source and applying a make-up volume of solvent for maintaining optimum spray conditions.

References

1. NIDA InfoFacts Sept. 2004 (www.drugabuse.gov)

2. US Dept. Of Justice Bureau of Justice Statistics (www.ojp.usdoj.gov/bjs/dcf/du.html)

3. DEA Briefs and Background, Drugs and Drug Abuse www.usdoj.gov/dea/concern/morphine.html


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