Navigation Links
Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS

Jason S. Wood, Ph.D. Varian, Inc.

Introduction

Testing for drugs of abuse (DoA) is becoming more prevalent in various environments, including forensics and social justice environments as well as in sporting events. Non-heroin opiates such as morphine, oxycodone, and hydrocodone are appearing increasingly in drug indicator data. For example, in San Francisco, the number of emergency department mentions of oxycodone overdose increased 110 percent in a one year peroid between 2001 and 20021. A US Department of Justice report from the Bureau of Justice Statistics in 2004 showed that the top four drugs utilized by high school seniors (and the percentage of those reporting their use in the last 12 months) were: 1) Alcohol (70.6%), 2) Marijuana (34.3%), 3) Stimulants (10.0%) and 4) Non-heroin Opiates (9.5%)2. Moreover, the US Drug Enforcement Administration (USDEA) reports that since 1990, there has been about a 3-fold increase in morphine products in the United States3.

This application note describes a rapid (5 min), dilute-and-shoot method for the determination of morphine and one of its major metabolites (morphine-glucuronide) in biological fluids (saliva and urine). This method has the advantage that it does not require lengthy derivatization processes (as in GC/MS) and, therefore, can be used in a high-throughput screen environment. It utilizes the advanced features of the Varian 500-MS to divert salts and contaminating proteins away from the API ion source and includes Varians patented OnTrak OraTube as a sampling mechanism for the saliva.

Instrumentation

Varian 500-MS LC Ion Trap Mass Spectrometer equipped with an ESI source

Two Varian ProStar 210 Solvent Delivery Modules

CTC Analytics HTS PAL AutoSampler

Materials and Reagents

All chemica
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:1/22/2015)... 22, 2015 Selexis SA, a serial ... Cell Banks (RCBs) used for drug discovery to commercial ... will include Next-Generation Sequencing (NGS) data packages. ... biologic manufacturing by ensuring the integrity of the gene, ...
(Date:1/22/2015)... Medical Systems (NYSE: VAR ), world leader in ... to sustainability with inclusion on a prestigious list of the ... equipment company among the Corporate Knights Global 100 Most Sustainable ... Davos, Switzerland . "We ...
(Date:12/24/2014)...  United Therapeutics Corporation (NASDAQ: UTHR ) announced ... has submitted a pre-market approval application to the U.S. ... Medtronic,s SynchroMed ® II implantable drug infusion system ... Therapeutics, Remodulin ® (treprostinil) Injection delivered intravenously to ...
(Date:12/24/2014)... 2014 “Preparative & Process Chromatography ... Consumables (Columns, Solvents, Buffers, Valves, Guages, Seals), Accessories, ... - Forecasts to 2019” provides a detailed overview ... market trends, and strategies impacting the preparative and ...
Breaking Biology Technology:Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2Varian Honored Among World's 100 Most Sustainable Corporations 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 3United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 4Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4
... announced that it has entered into agreement with ... generated by 100 percent,renewable energy resources, through IPL,s ... of fossil-fueled power generation, this,program will better enable ... BST becomes the third-largest customer to participate in ...
... objective of the,Alliance is to improve patient care ... marketplace. To celebrate the Alliance, a symposium ... with noted speakers from industry, government,entrepreneurs, venture capitalists ... Gesellschaft. The alliance will take advantage of ...
... in Pioneering Field, BETHESDA, Md., Sept. 10 ... of an ownership interest in,ClinResearch GmbH, a global ... adaptive, clinical trials. Headquartered in Cologne, Germany,with more ... 80,adaptive programs over the past seven years. ClinResearch,s ...
Cached Biology Technology:BioStorage Technologies Becomes Green Powered through the Use of 100% Certified Renewable Energy 2BioStorage Technologies Becomes Green Powered through the Use of 100% Certified Renewable Energy 3BioStorage Technologies Becomes Green Powered through the Use of 100% Certified Renewable Energy 4'Alliance for Medical Devices, Instrumentation and Diagnostics' formed between Fraunhofer Center for Manufacturing Innovation and Boston University 2United BioSource Acquires Interest in Leading Adaptive Design Technology Service Provider 2
(Date:12/17/2014)... Valencell, a leader in performance biometric data sensor technology, ... such as Intel, Jabra, Atlas, and Scosche to develop ... products will be showcased at CES 2015, taking place ... "Our partners choose PerformTek biometrics, because ...
(Date:12/17/2014)... DENVER , Dec. 16, 2014  Automation is fundamentally ... evident than at international borders. Over the past decade, ... veteran travelers to self process through border control via ... increasing number of airports, seaports, and land borders across ...
(Date:12/11/2014)... Research and Markets , ... announced the addition of the "Biometrics Market in ... One major trend emerging in this market ... it is important to upgrade biometric solutions to the ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... outbreaks, wildfire, and forest dieback, may adversely affect people ... The U.S. Geological Survey led a new ... "ecological thresholds" in North America. The report, which was ... authored by a team of federal and academic climate ...
... expert David S. Rose will present a lecture at ... You Think You Know...Is Wrong: How New Technologies and ... As part of the Heath Lecture Series at ... will discuss the latest technologies and concepts that are ...
... YORK, January 16, 2009 - Researchers at the University of ... adult beta cells have the ability to replicate with the ... was led by Andrew F. Stewart, M.D., a Professor of ... at the University of Pittsburgh School of Medicine. The ...
Cached Biology News:David Rose to present Howe School lecture on new technologies and business models, Jan. 29 2JDRF-funded researchers discover proteins regulating human beta cell replication 2
No description available in Ensembl Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
No description available in Ensembl. Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
12(S)-HETE-d8 is used as an internal standard for the quantification of 12(S)-HETE by stable isotope dilution mass spectrometry....
...
Biology Products: