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Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

hikimate 3-phosphate synthase in atomic details. Proc Natl Acad Sci 98(4):1376-1380 (2001).

2. Padgette, S.R., Biest Re, D., Gasser, C.S., Eichholtz, D.A., Frazier, R.B., Hironaka, C.M., Levine, E.B., Shah, D.M., Fraley, R.T. and Kishore, G.M. Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site. Journal of Biological Chemistry 266(33):22364-22369 (1991).

3. Barry, G., Kishore, G., Padgette, S. et al. Inhibitors of amino acid biosynthesis: strategies for imparting glyphosate tolerance to crop plants. Curr Topics Plant Physiol 7:139-145 (1992).

4. Ahmed, F.E. Detection of genetically modified organisms in foods. Trends Biotechnol 20(5):215-223 (2002).

5. Tengel. C., Schler, P., Setzke, E., Balles, J. and Sprenger-Hauels, M. PCR-based detection of genetically modified soybean and maize in raw and highly processed foodstuffs. BioTechniques 31(2):426-429 (2001).

6. Amutan, M. and Batey, D. Real-time quantification of genomic DNA using DyNAzyme II DNA polymerase and SYBR Green I dye. Application Note Vol. 1, No. 1. MJ Research, Inc.


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