Navigation Links
Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

on of glyphosate to fields of the growing GMO crop, e.g., Roundup Ready soybeans.

Because GMOs contain specific and well-defined nucleic acid sequences, they are well suited for detection by the polymerase chain reaction (PCR). Indeed, PCR has been shown to effectively detect GMO material, using an agarose gel analysis of the reaction products.4, 5

In this Application Note, we extend the PCR detection of GMOs by presenting a real-time qPCR protocol using SYBR Green I (SGI) performed on the DNA Engine Opticon 2 continuous fluorescence detection system. DNA was isolated from reference materials containing known proportions of GMO soy, as well as from soy products obtained from a local supermarket. Control primers that amplified a portion of the soy lectin gene were used to normalize the amount of soy DNA in each sample. A second set of primers that amplify a portion of the glyphosate-tolerant EPSPS gene were used for GMO detection.

The threshold cycle [C(t)] of a reaction is defined as the point when a reaction signal just rises over the background signal level. Comparing the relative C(t)s of lectin and EPSPS reactions from the GMO concentration reference series allowed the construction of a standard curve, from which the amount of GMO materials in unknown foodstuffs could be determined down to approximately 0.5% (w/w) concentration.


Materials and Methods
Isolation of DNA from Reference Standards and Food Samples
Dried soybean powder containing mass fractions of 0% to 5% Roundup Ready soybean (RTC Corporation, IRMM-41050-56) were used as reference standards. The reference standards were prepared by the manufacturer by mixing dried non-genetically modified and
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:8/4/2015)...  Blueprint Medicines (NASDAQ: BPMC ), a leader ... genomically defined diseases, today announced that Jeffrey Albers ... at the 2015 Wedbush PacGrow Healthcare Conference on Tuesday, ... A live webcast of the event will be available ... website at www.blueprintmedicines.com . An archived replay of ...
(Date:8/4/2015)... Aug. 4, 2015 /PRNewswire/ -- Sinovac Biotech Ltd. (Nasdaq: ... China , today announced that it ... quarter, ended June 30, 2015, after market close on ... call on Thursday, August 13, 2015, at 8:00 a.m. ... Standard Time) to review the Company,s financial results and ...
(Date:8/3/2015)... Aug. 3, 2015  MiMedx Group, Inc. (NASDAQ: ... amniotic tissue and patent-protected processes to develop and market ... Orthopedic, Spine, Sports Medicine, Ophthalmic and Dental sectors of ... 35 th Annual Canaccord|Genuity Growth Conference in ... Chairman and CEO, William C. Taylor , President ...
(Date:8/3/2015)... 2015  Aethlon Medical, Inc. (Nasdaq: AEMD ), ... life-threatening diseases, today announced that it will host its ... on Thursday, August 13, 2015 at 4:30 p.m. Eastern ... the release of fiscal 2016 first quarter results.  A ... two hours after the call at the Investor Relations ...
Breaking Biology Technology:Sinovac to Host Conference Call to Report Second Quarter 2015 Unaudited Financial Results 2MiMedx To Present At 35th Annual Canaccord|Genuity Growth Conference 2Aethlon Medical To Host First Quarter Fiscal 2016 Investor Call on August 13, 2015 2
... Ind., June 30 Zimmer Holdings, Inc. (NYSE: ZMH ... earnings conference call will be broadcast live over the Internet on ... news release detailing the quarterly results will be made available at ... The live audio webcast can be accessed via Zimmer,s Investor Relations ...
... 30 In connection with its proposed acquisition of London ... June 5 2009, Inverness Medical Innovations, Inc. (NYSE: ... to remind Inverness shareholders through this announcement of their dealing ... , , Dealing disclosure requirements of the UK ...
... Quarter 2009 Earnings Conference Call Tuesday, August 4, 2009, 5:00 p.m. U.S. ... (Nasdaq: CEPH ) has scheduled its regular quarterly earnings conference call covering ... , , August 4, 2009 Schedule , , ... PRNewswire, 4:50 p.m. EDT To participate in the conference ...
Cached Biology Technology:Inverness Medical Innovations - Concateno plc, Further Announcement Regarding UK Takeover Code Share Dealing Disclosure Requirements. 2
(Date:7/21/2015)... , July 21, 2015  NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile ... wallet, announces that it has filed provisional patent ... Method. This invention highlights next ... authorizes an account, but also the user using ...
(Date:7/13/2015)... 2015 Synaptics Inc. (NASDAQ: SYNA ... today announced sampling of ClearPad ® 4300, ... (TDDI) product targeting smartphones and tablets. This new ... , best-in-class touch controller IP and systems ... in the company,s Japan Design Center. TDDI enables ...
(Date:7/9/2015)... 2015 Research and ... the "Biometrics for Banking; Market & Technology ... to their offering. The adoption for ... growth and the forecast is that by 2020 ... companies involved in delivering biometric systems to the ...
Breaking Biology News(10 mins):NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 2NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 4Synaptics Announces Sampling of Second Generation TDDI Solutions 2Synaptics Announces Sampling of Second Generation TDDI Solutions 3Global Banking Biometrics Market 2015-2020 - Market & Technology Analysis, Adoption Strategies and Forecasts 2Global Banking Biometrics Market 2015-2020 - Market & Technology Analysis, Adoption Strategies and Forecasts 3
... Oct. 18 Reportlinker.com announces that a new ... Global Biometrics Industry ... report analyzes the worldwide markets for Biometrics in ... (Automated Fingerprint Identification System), Non-AFIS/Finger Scan, Hand Geometry, ...
... the MPI of Biochemistry and GSK pursues the objective ... development work is based on the research findings of ... and fellow scientist Mathias Bcker successfully described the role ... in connection with the development of type II diabetes, ...
... change on the microbial communities of two important ecosystemsthe ... a University of Oklahoma research group has been awarded ... to Jizhong Zhou, OU professor of botany and microbiology ... results of these studies could potentially contribute to the ...
Cached Biology News:Reportlinker Adds Global Biometrics Industry 2Reportlinker Adds Global Biometrics Industry 3Reportlinker Adds Global Biometrics Industry 4Reportlinker Adds Global Biometrics Industry 5Reportlinker Adds Global Biometrics Industry 6Reportlinker Adds Global Biometrics Industry 7Reportlinker Adds Global Biometrics Industry 8Reportlinker Adds Global Biometrics Industry 9Reportlinker Adds Global Biometrics Industry 10Reportlinker Adds Global Biometrics Industry 11Reportlinker Adds Global Biometrics Industry 12Reportlinker Adds Global Biometrics Industry 13Reportlinker Adds Global Biometrics Industry 14Reportlinker Adds Global Biometrics Industry 15Reportlinker Adds Global Biometrics Industry 16Reportlinker Adds Global Biometrics Industry 17Reportlinker Adds Global Biometrics Industry 18Reportlinker Adds Global Biometrics Industry 19Reportlinker Adds Global Biometrics Industry 20Reportlinker Adds Global Biometrics Industry 21Reportlinker Adds Global Biometrics Industry 22Reportlinker Adds Global Biometrics Industry 23Cooperation in diabetes research 2
... The European Collection of Cell Cultures (ECACC) ... from industry, academia and government institutes for ... I course designed to introduce candidates to ... which focuses on more specialist areas such ...
...
These 22x40mm rectangular No.1 Corning cover glasses are made from No. 0211 zinc titania glass and are 0.16 to 0.19mm thick. • Approx. Pcs Oz : 73...
...
Biology Products: