Navigation Links
Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

on of glyphosate to fields of the growing GMO crop, e.g., Roundup Ready soybeans.

Because GMOs contain specific and well-defined nucleic acid sequences, they are well suited for detection by the polymerase chain reaction (PCR). Indeed, PCR has been shown to effectively detect GMO material, using an agarose gel analysis of the reaction products.4, 5

In this Application Note, we extend the PCR detection of GMOs by presenting a real-time qPCR protocol using SYBR Green I (SGI) performed on the DNA Engine Opticon 2 continuous fluorescence detection system. DNA was isolated from reference materials containing known proportions of GMO soy, as well as from soy products obtained from a local supermarket. Control primers that amplified a portion of the soy lectin gene were used to normalize the amount of soy DNA in each sample. A second set of primers that amplify a portion of the glyphosate-tolerant EPSPS gene were used for GMO detection.

The threshold cycle [C(t)] of a reaction is defined as the point when a reaction signal just rises over the background signal level. Comparing the relative C(t)s of lectin and EPSPS reactions from the GMO concentration reference series allowed the construction of a standard curve, from which the amount of GMO materials in unknown foodstuffs could be determined down to approximately 0.5% (w/w) concentration.


Materials and Methods
Isolation of DNA from Reference Standards and Food Samples
Dried soybean powder containing mass fractions of 0% to 5% Roundup Ready soybean (RTC Corporation, IRMM-41050-56) were used as reference standards. The reference standards were prepared by the manufacturer by mixing dried non-genetically modified and
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:9/20/2014)... HealthTronics, Inc. , a leading ... and services, has signed a distribution agreement through ... for its high precision laser technology. ... mobile, surgical technology—offering more choices for healthcare providers ... will make available Clinicon’s FDA-cleared SureLase™ CO2 Laser ...
(Date:9/19/2014)... 19, 2014  InterMune, Inc. (Nasdaq: ITMN ... of Understanding to settle the litigation in the Delaware ... of Merger dated as of August 22, 2014, among ... As one term of the Memorandum of Understanding, the ... in the attached exhibit reflecting the amendment to the ...
(Date:9/19/2014)... at NYU Langone Medical Center have found a way ... for turning adult cells into so-called pluripotent stem cells ... the new technique in mice, the researchers increased the ... by more than 20-fold compared with the standard method. ... thus should generally accelerate research aimed at using stem ...
(Date:9/19/2014)... 19, 2014 Follow us ... to revolutionize the world has attracted diverse players ... small start-ups. The early years of nanotechnology development ... due to the high risks involved, which deterred ... for this evolving technology have been growing over ...
Breaking Biology Technology:HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 2HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 3InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 2InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 3InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 4InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 5InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 6InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 7InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 8InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 9InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 10InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 11InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 12InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 13InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 14InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 15InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 16InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 17InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 18InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 19NYU Langone scientists report reliable and highly efficient method for making stem cells 2NYU Langone scientists report reliable and highly efficient method for making stem cells 3Growing R&D Investments & Developments in Nanomaterials to Drive the Global Nanotechnology Market, According to New Report by Global Industry Analysts, Inc. 2Growing R&D Investments & Developments in Nanomaterials to Drive the Global Nanotechnology Market, According to New Report by Global Industry Analysts, Inc. 3Growing R&D Investments & Developments in Nanomaterials to Drive the Global Nanotechnology Market, According to New Report by Global Industry Analysts, Inc. 4
... an advanced,biomaterials company, announced today that it has ... Under the agreement, LifeNet Health will provide ... mixed with ETEX,s proprietary,calcium phosphate to form a ... EquivaBone(TM) in its Cambridge,facilities and market the product ...
... Corporation,(Nasdaq: IOMI ) today announced that it will ... markets open and hold a,conference call and live webcast ... 2008 at 1:30 p.m. Eastern Time., Conference Call ... Thursday, March 6 at 1:30 p.m.,Eastern Time via phone, ...
... /PRNewswire/ - BIKAM,pioneers in the discovery and ... of degenerative eye diseases and ocular disorders ... exclusive worldwide,collaboration and agreement to discover and ... Dalton Medicinal Chemistry will utilize their ...
Cached Biology Technology:ETEX and LifeNet Health, Inc. Enter Agreement 2Iomai to Announce Fourth Quarter and Year-End 2007 Financial Results and Host Webcast on Thursday, March 6 2BIKAM and Dalton Medicinal Chemistry Sign Collaborative Research Agreement 2
(Date:9/19/2014)... it the Jimmy Durante of dinosaurs a ... profile. The new dinosaur, named Rhinorex condrupus ... and Brigham Young University, lived in what is ... the Late Cretaceous period. , Rhinorex, which translates ... a close relative of other Cretaceous hadrosaurs like ...
(Date:9/19/2014)... A new resource unveiled today by researchers from ... College Dublin provides both experienced and aspiring researchers ... build, and operate robots made from soft, flexible ... printing, laser cutters, and other advances in manufacturing ... important field. Using principles drawn from conventional rigid ...
(Date:9/19/2014)... will study the unique bioelectric signaling system of the ... health. The OU researcher is working to understand ... the signals used to map the world around them. ... of 500-600 discharges a second throughout their lives. ... extreme, but necessary for survival. , "There is evidence ...
Breaking Biology News(10 mins):New hadrosaur noses into spotlight 2Soft robotics 'toolkit' features everything a robot-maker needs 2
... a pretzel factory production line, with conveyer belts carrying the dough, ... Now imagine what would happen if pretzel dough started ... conveyor, misshapen, and sticking fast to the dough of the other ... mess could no longer be conveyed into the oven, the backup ...
... CRUZ, CA--An intricate three-way mating struggle first observed in ... in a distant relative, the European common lizard. The ... million years of evolution, yet they share behavioral and ... the males, according to new research published in the ...
... the University of Warwick have devised a means of ... in just a few seconds. Previous techniques have ... finger print and laboriously match them against a database ... entire detailed pattern of each print and transform the ...
Cached Biology News:Botched production of insulin molecule may lead to diabetes 2Botched production of insulin molecule may lead to diabetes 3Botched production of insulin molecule may lead to diabetes 4Three-way mating game of North American lizard found in distant European relative 2Three-way mating game of North American lizard found in distant European relative 3Three-way mating game of North American lizard found in distant European relative 4New technology identifies warped fingerprints at warp speed 2
... For use in Refrigerated Vapor Trap ... Vacuum Source (UVS) Models UVS400, UVS400A and ... polymer suitable for use for temperatures ranging ... has excellent thermal stability and does not ...
Acetate Plate Sealers 100/Box...
... demands the detection of very small amounts ... AXIMA-CFRplus with improved resolution (20,000) and mass ... confidence in protein identification. The AXIMA-CFRplus also ... sample target handling and the ability to ...
QCPN...
Biology Products: