Navigation Links
Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

Surekha Karudapuram, Ph.D. and David Batey, Ph.D.
MJ Research, Inc., South San Francisco, CA


Abstract
We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to be resistant to the herbicide glyphosate. The assay utilizes SYBR Green I (SGI) detection of amplicons of the genetically modified organism (GMO) specific sequences as well as a common soy gene as a reference. The assay can detect GMO materials down to 0.5% (w/w) concentration in foods. The use of SGI for detection makes this protocol readily adaptable for the analysis of other food crops for the presence of GMO material.


Introduction
A wide variety of food crops have been genetically engineered to contain beneficial traits such as herbicide resistance, disease resistance and insect resistance. Accurate and reliable detection of genetically modified organisms (GMOs) in foods is becoming increasingly important as the demand for labeling of GMO-containing foods increases.

Perhaps the most widely utilized modification that confers herbicide resistance is that which confers resistance to glyphosate [N- (phosphonomethyl)glycine]. Glyphosate is a competititive inhibitor of enolpyruval-shikimate-3-phosphate synthase (EPSPS), which is required for the production of aromatic amino acids by plants.1

To create glyphosate resistance, plants are transformed with a glyphosate tolerant version of the EPSPS gene from strain CP4 of the soil bacterium Agrobacterium sp.2, 3 Expression of the CP4 EPSPS in such modified plants confers resistance to glyphosate, and thus allows the applicati
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:10/31/2014)... Brady (NYSE:BRC), a global ... a new video testimonial on the effectiveness ... a research technician at the BloodCenter of Wisconsin discusses ... and storing hundreds of thousands of samples, and how ... , “At Brady, we design our label materials with ...
(Date:10/31/2014)... 2014  Tavistock Development Co. (TDC), a diversified ... groundbreaking of the GuideWell Innovation Center ... The 92,000-square-foot, three-story facility is the first multi-tenant ... designed specifically for life sciences, health innovation, and ... 650-acre Lake Nona Medical City, near the Sanford-Burnham ...
(Date:10/30/2014)... Investor-Edge has initiated coverage on the following ... (NASDAQ: MDVN ), Pharmacyclics Inc. (NASDAQ: ... and Ironwood Pharmaceuticals Inc. (NASDAQ: IRWD). Free research on ... . On Wednesday, October 29, 2014, the ... Jones Industrial Average declined 0.18%, to finish the day ...
(Date:10/30/2014)... Dallas, Texas (PRWEB) October 30, 2014 ... China Naltrexone HCL Industry” is a professional and ... The report provides basic Naltrexone HCL information, including ... as well as industry overview. This research covers ... as well as global industry analysis covering macroeconomic ...
Breaking Biology Technology:Brady Debuts New Lab Label Testimonial Video 2GuideWell Innovation Center Groundbreaking Hosted by Tavistock Development Company at Lake Nona Medical City 2GuideWell Innovation Center Groundbreaking Hosted by Tavistock Development Company at Lake Nona Medical City 3Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 2Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 3Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 4Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 5Naltrexone HCL Market & Nalbuphine HCL Industry Analysis for Global, China Regions Now at DeepResearchReports.com 2Naltrexone HCL Market & Nalbuphine HCL Industry Analysis for Global, China Regions Now at DeepResearchReports.com 3
... , , ... software for the analysis of RNA, DNA, proteins, and cells with the ... , , , , ... tool for RNA,DNA proteins and cells., , , ...
... , , ... , , , This Application ... apoptotic cell samples. Performance of two assays,for apoptosis detection is demonstrated ... cytometer. Histogram quality,of the low number of fluorescent cells counted with ...
... , , ... , , , ... become an essential tool in any,laboratory working on cell-based studies. ... also the determination of growth factor,and cytokine activity. Even more ...
Cached Biology Technology:2100 expert software , Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer 2Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 2Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 3Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 4Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 5Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 6Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 7Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 8Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 9Cell Proliferation and Viability Measurement 2Cell Proliferation and Viability Measurement 3Cell Proliferation and Viability Measurement 4
(Date:10/30/2014)... , Oct. 29, 2014  Securus ... criminal justice technology solutions for public safety, ... it has deployed exciting enhancements to its ... analytics to provide actionable intelligence and focused ... continues to drive technological innovation through identifying ...
(Date:10/29/2014)... is open for the Society of ... SIR 2015 is the one place where ... related health care professionals can come together ... and technologies being presented, discussed and tested. ... minimally invasive, image-guided medicine, SIR 2015 will ...
(Date:10/29/2014)... ceremony held in New York City on September 19, ... American Cancer Society Professor of Genome Sciences and Medicine ... Honorary Doctor of Philosophy of Tel Aviv University, was ... Science. , In addition to her honorary degree from ... the TAU School of Medicine, and for the past ...
Breaking Biology News(10 mins):Securus Technologies Adds Advanced Features to Its Industry-Leading Investigative Analytics Product 2SIR 2015: Interventional radiology shapes tomorrow's medicine 2Geneticist and humanitarian: Prof. Mary-Claire King receives Lasker Award 2
... and Technology (NIST) spent 75 days on the job ... dwelling" of the release, distribution and fate of particles ... DNA molecule. Particles ranging in size from 100 nanometers ... and electric stoves, hair dryers, power tools and candles ...
... Philadelphia, PA, -- MicroRNAs (miRNAs) that regulate processes including ... disease. They can be collected from routinely collected ... a number of factors can interfere with the accuracy ... today in the Journal of Molecular Diagnostics , ...
... for females to avoid unwanted male attention: choosing more attractive ... of the Royal Society B, the study is the ... attractive than themselves to reduce harassment from males. Carried ... focuses on the Trinidadian guppy, a species of small freshwater ...
Cached Biology News:Elusive ultrafine indoor air contaminants yield to NIST analysis 2New protocols improve detection of microRNAs for diagnosis 2New protocols improve detection of microRNAs for diagnosis 3Females choose sexier friends to avoid harassment 2