Navigation Links
Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

Surekha Karudapuram, Ph.D. and David Batey, Ph.D.
MJ Research, Inc., South San Francisco, CA


Abstract
We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to be resistant to the herbicide glyphosate. The assay utilizes SYBR Green I (SGI) detection of amplicons of the genetically modified organism (GMO) specific sequences as well as a common soy gene as a reference. The assay can detect GMO materials down to 0.5% (w/w) concentration in foods. The use of SGI for detection makes this protocol readily adaptable for the analysis of other food crops for the presence of GMO material.


Introduction
A wide variety of food crops have been genetically engineered to contain beneficial traits such as herbicide resistance, disease resistance and insect resistance. Accurate and reliable detection of genetically modified organisms (GMOs) in foods is becoming increasingly important as the demand for labeling of GMO-containing foods increases.

Perhaps the most widely utilized modification that confers herbicide resistance is that which confers resistance to glyphosate [N- (phosphonomethyl)glycine]. Glyphosate is a competititive inhibitor of enolpyruval-shikimate-3-phosphate synthase (EPSPS), which is required for the production of aromatic amino acids by plants.1

To create glyphosate resistance, plants are transformed with a glyphosate tolerant version of the EPSPS gene from strain CP4 of the soil bacterium Agrobacterium sp.2, 3 Expression of the CP4 EPSPS in such modified plants confers resistance to glyphosate, and thus allows the applicati
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:1/22/2015)... , Jan. 22, 2015  Transwestern | RBJ today announces ... of office space for Shire a leading biopharmaceutical company, at ... Transwestern | RBJ,s Robert Richards , president, and ... lease for the entire five-floor building at 95 Hayden Ave. ...
(Date:1/22/2015)... 22, 2015 Pipette.com has added the ... their comprehensive portfolio of Eppendorf products. , The Eppendorf ... a Centrifuge 5424/5424 R and receive the following:, ... Research plus or Eppendorf Reference 2 , ...
(Date:1/22/2015)... 2015 Diagenode, Inc., a leading ... Bioruptor® and complete solutions for epigenetics research, recently ... alleviating the need for manual processing. The new ... ChIP of histones or transcription factors and a ...
(Date:1/22/2015)...  Derma Sciences, Inc. (Nasdaq: DSCI ), ... care, announces that AMNIOEXCEL® and AMNIOMATRIX®, amniotic allografts ... the Premier, Inc. Regenerative Skin Grafting contract portfolio. ... and AMNIOMATRIX® product lines, which continue to be ...
Breaking Biology Technology:Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5
... ... line of products that leverages the same improvements as the recently released 25mm and 36mm ... engineered plastics, and larger ball bearings for greater rotor support and high axial loading capability. ... Waterbury, CT ...
... magnetic fields to drive iron-bearing nanoparticles to metal stents ... drug payload that successfully prevents blockages in those vessels. ... results at a lower dose than conventional non-magnetic stent ... research is the latest in a series of studies ...
... Vienna, Austria, Friday 16 April: Results of a German ... 2010, the Annual Meeting of the European Association for ... that overall, treatment with high dose (23-28mg/kg/d) ursodeoxycholic acid ... treatment of non-alcoholic steatohepatitis (NASH), the most advanced form ...
Cached Biology Technology:Haydon Kerk Motion Solutions, Inc. Introduces the New 20mm Stepper Motor Linear Actuator 2Haydon Kerk Motion Solutions, Inc. Introduces the New 20mm Stepper Motor Linear Actuator 3Magnetic fields drive drug-loaded nanoparticles to reduce blood vessel blockages in an animal study 2Magnetic fields drive drug-loaded nanoparticles to reduce blood vessel blockages in an animal study 3Magnetic fields drive drug-loaded nanoparticles to reduce blood vessel blockages in an animal study 4High dose UDCA therapy does not improve overall liver histology in obesity related hepatitis 2
(Date:12/19/2014)... ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has announced the addition of ... Market" report to their offering. ... market is estimated to grow at a CAGR of 3.6% ... larger share in this market, Canada ...
(Date:12/17/2014)... , Dec. 15, 2014 The Defense Logistics ... to detect and prevent counterfeit microcircuits from entering into ... performing an in-house microcircuit anti-counterfeit initiative dubbed DNA marking. ... while increasing their reliability throughout the supply chain. The ...
(Date:12/17/2014)... 16, 2014  Automation is fundamentally transforming the travel experience ... borders. Over the past decade, ePassports, biometric readers, and ... process through border control via eGates and Automated Passport ... seaports, and land borders across the globe. ...
Breaking Biology News(10 mins):Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2
... from their voices is second nature to humans. Certain ... live in social groups are also able to do ... is vital for meerkats. They coordinate their activities with ... approaching predators, for instance, and thus stick together as ...
... researchers has shown that female kittens consume more food and ... entire littermates. This research was conducted at the WALTHAM Centre ... brands such as WHISKAS and ROYAL CANIN. In a ... more food than their entire littermates for an 18 week ...
... German scientists discovered that some strains of algae, when starved ... war ended, and the discovery lay dormant until people began ... In the early 2000s, algal fuel production enjoyed a ... the notion of converting "pond scum" to oil. But a ...
Cached Biology News:Meerkats recognize each other from their calls 2New research from WALTHAM demonstrates the importance of portion control for maintaining healthy body weight in neutered female kittens 2WUSTL wins $ 2.2 million US Department of Energy grant to build a fuel producing bacterium 2WUSTL wins $ 2.2 million US Department of Energy grant to build a fuel producing bacterium 3WUSTL wins $ 2.2 million US Department of Energy grant to build a fuel producing bacterium 4
... anti-phospho-FAK (Ser843) ... acid region encompassing the human, mouse, ... Accession Number: NM_153831 ... Routinely evaluated by immunoblot. ...
Molecular Biology grade. Ideally suited for DNA and RNA separation and recovery. High gel strength. Gelling temp. 36C. Melting temp. 88C. EEO: 0.05-0.13 Resolution 0.2-40 kb; Rnase/Dnase: none detec...
Rabbit polyclonal to Quinolinic acid ( Abpromise for all tested applications). Antigen: Synthetic quinolinic acid conjugated to bovine serum albumin....
Phospho-YAP (Ser127) Antibody Ship: Hot Store: -20 C...
Biology Products: