Navigation Links
Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

Surekha Karudapuram, Ph.D. and David Batey, Ph.D.
MJ Research, Inc., South San Francisco, CA


Abstract
We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to be resistant to the herbicide glyphosate. The assay utilizes SYBR Green I (SGI) detection of amplicons of the genetically modified organism (GMO) specific sequences as well as a common soy gene as a reference. The assay can detect GMO materials down to 0.5% (w/w) concentration in foods. The use of SGI for detection makes this protocol readily adaptable for the analysis of other food crops for the presence of GMO material.


Introduction
A wide variety of food crops have been genetically engineered to contain beneficial traits such as herbicide resistance, disease resistance and insect resistance. Accurate and reliable detection of genetically modified organisms (GMOs) in foods is becoming increasingly important as the demand for labeling of GMO-containing foods increases.

Perhaps the most widely utilized modification that confers herbicide resistance is that which confers resistance to glyphosate [N- (phosphonomethyl)glycine]. Glyphosate is a competititive inhibitor of enolpyruval-shikimate-3-phosphate synthase (EPSPS), which is required for the production of aromatic amino acids by plants.1

To create glyphosate resistance, plants are transformed with a glyphosate tolerant version of the EPSPS gene from strain CP4 of the soil bacterium Agrobacterium sp.2, 3 Expression of the CP4 EPSPS in such modified plants confers resistance to glyphosate, and thus allows the applicati
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:7/30/2015)... ... July 31, 2015 , ... Ralco is honored to announce ... Lyon County Fair to be held August 5-9 in Marshall. The Ralco Enrichment Center ... come from and how agriculture impacts their daily lives. This unique exhibit also features ...
(Date:7/30/2015)... Washington , July 30, 2015 ... Market, by Product (Software, Molecular Modeling, PB/PK, PKPD, Trial ... Discovery & Development, Preclinical, Clinical Trial), by End Users ... Biosimulation Market is expected to reach USD 2,107.99 million ... a CAGR of 15.29%. Browse more ...
(Date:7/30/2015)... 2015 Ascendis Pharma A/S (Nasdaq: ... innovative TransCon technology to address significant unmet medical ... six-month Phase 2 study to evaluate the safety ... 53 treatment-naïve, pre-pubertal children with growth hormone deficiency, ... with the top-line results from our Phase 2 ...
(Date:7/29/2015)... 2015 Sanofi, a global ... second quarter of 2015. CEO Olivier Brandicourt ... performance in different businesses. Watch video ... Topics covered in the interview ... - Performance drivers - Diabetes ...
Breaking Biology Technology:Ralco Announces Primary Sponsorship of Ralco Enrichment Center at Lyon County Fair 2Biosimulation Market Worth 2,107.99 Million USD by 2020 2Biosimulation Market Worth 2,107.99 Million USD by 2020 3Biosimulation Market Worth 2,107.99 Million USD by 2020 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 5Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 6Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 7
... of the equation , ,Identity theft has seen the ... three years. The United States is faced with an ever-changing ... both sides of the equation, organizational and consumer. , ... to a new report from the Federal Trade Commission (FTC). ...
... pioneer company looks to reclaim lost ground , ,CHIPPEWA FALLS, ... operating out of offices on Lowater Road here in its hometown, ... wonderful heights. , ,With its next-generation X1, on the market since ... toward the future with a twinkle in its corporate eye that, ...
... often been regarded as the economic motor of the European ... of Scandinavia, to the east of the U.K. and France, ... the east European countries of Poland, the Czech Republic and ... not only one of Europes largest populations but considering ...
Cached Biology Technology:Are You Ready for Identity Theft? 2Are You Ready for Identity Theft? 3Are You Ready for Identity Theft? 4Are You Ready for Identity Theft? 5Are You Ready for Identity Theft? 6Are You Ready for Identity Theft? 7Cray Rides Again 2Cray Rides Again 3German Life Science Oktoberfest Comes to the Midwest 2German Life Science Oktoberfest Comes to the Midwest 3German Life Science Oktoberfest Comes to the Midwest 4German Life Science Oktoberfest Comes to the Midwest 5German Life Science Oktoberfest Comes to the Midwest 6
(Date:7/23/2015)... (NASDAQ: AWRE ), a leading supplier of biometrics software ... ended June 30, 2015.  Revenue for the ... 33% compared to $6.8 million in the same quarter last ... $0.3 million, or $0.01 per diluted share, which compared to ... period a year ago.  Lower revenue and ...
(Date:7/21/2015)... Passwords have proven futile for securing ... U.S. Office of Personnel Management breach. Biometric authentication ... but developers and end-users are concerned about how ... Biometrics-as-a-Service provider HYPR Corp. announced today the general ... party integration. A video overview ...
(Date:7/9/2015)... 9, 2015  Synaptics Inc. (NASDAQ: SYNA ), a ... it will report financial results for the fourth quarter ... after the close of market. The company will host ... 2:00 p.m. PT (5:00 p.m. ET), during which management ... on the live call, analysts and investors should dial ...
Breaking Biology News(10 mins):Aware, Inc. Reports Second Quarter 2015 Financial Results 2Aware, Inc. Reports Second Quarter 2015 Financial Results 3Aware, Inc. Reports Second Quarter 2015 Financial Results 4Aware, Inc. Reports Second Quarter 2015 Financial Results 5Aware, Inc. Reports Second Quarter 2015 Financial Results 6HYPR Corp. Launches Industry First Biometric Tokenization Platform 2HYPR Corp. Launches Industry First Biometric Tokenization Platform 3HYPR Corp. Launches Industry First Biometric Tokenization Platform 4Synaptics to Report Fourth Quarter, Fiscal Year 2015 Results on July 30 2
... hundreds, if not thousands, of suspected toxic substances every ... the brain, immune system, reproductive organs or hormones. Children ... requires product testing of most chemical compounds before they ... Riverside professor Carl Cranor argues in a new book, ...
... research effort designed to prevent the introduction of viruses to ... Chesapeake Bay watermen improve their bottom line by reducing the ... The findings, published in the journal Diseases of Aquatic ... in diseased and dying crabs likely occurs after the pre-molt ...
... scientists of the Technische Universitaet Muenchen (TUM) have been ... Prof. Stephan A. Sieber for the development of drugs ... million to study interactions between cellular components; 1.6 million ... a basic theory on elementary particles; Prof. Daniel Cremers ...
Cached Biology News:Legally poisoned 2Blue crab research may help Chesapeake Bay watermen improve soft shell harvest 2Nearly 10 million euros ($13.6 million) in ERC grants for 6 Technische Universitaet Muenchen researchers 2Nearly 10 million euros ($13.6 million) in ERC grants for 6 Technische Universitaet Muenchen researchers 3Nearly 10 million euros ($13.6 million) in ERC grants for 6 Technische Universitaet Muenchen researchers 4Nearly 10 million euros ($13.6 million) in ERC grants for 6 Technische Universitaet Muenchen researchers 5Nearly 10 million euros ($13.6 million) in ERC grants for 6 Technische Universitaet Muenchen researchers 6
... to ARG. Vasopressin The antibody ... Cells are found in the paraventricular ... found throughout the hypothalmus in rat ... Immunogen: Arginine ...
Rabbit polyclonal to LCAT ( Abpromise for all tested applications). entrezGeneID: 3931 SwissProtID: P04180...
Mouse monoclonal [7E10] to C Peptide ( Abpromise for all tested applications). entrezGeneID: 3630 SwissProtID: P01308...
... peptide transporter 2 (APT2) ... transporter PSF2) (Peptide supply ... transporter involved in antigen ... Antigen: ...
Biology Products: