Navigation Links
Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting

O. Rönn1, M. Landström2, A. Edman-Örlefors1, and S. Edlund1
1 GE Healthcare, Uppsala, Sweden
2 Ludwig Institute for Cancer Research, Uppsala, Sweden


ECL Plex™ Western Blotting Detection System can detect proteins at picogram levels, with linearity over 3.6 orders of magnitude. Two antigens can be detected simultaneously, and differential quantitation of low-abundant proteins, such as phosphoproteins in cell lysates, can be performed.


Introduction
ECL Plex Western blotting detection system is based on direct fluorescent light detection from CyDye™ labeled antibody conjugates. The use of different fluorophores, Cy3 and Cy5, enables simultaneous detection of multiple antigens on the same blotted membrane (Fig 1). This eliminates possible loss of material from a stripping procedure, providing more accurate data. Together with a high-end imaging system (e.g. Typhoon™ scanner or Ettan™ DIGE Imager), this results in high linearity and broad dynamic range for superior detection and quantitation of low-abundant proteins.


Methods
Pure proteins or cell extracts were run on 12% Trisglycine gels. After electrophoresis, gels were blotted onto a Hybond™ ECL or Hybond-LFP membrane, and then probed with primary antibodies and secondary CyDyeconjugated antibodies according to the manufacturer’s instructions. Please see the application note Multiplex protein detection using the ECL Plex fluorescent Western blotting system (28-4015-40) (1), available at www.amershambiosciences.com, for detailed methods.


Results and discussion
Sensitivity and dynamic r
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A New PCR-based Mycoplasma Detection Method
3. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
6. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
7. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. In Situ Cell Death Detection Kit
Post Your Comments:
(Date:7/31/2014)... July 31, 2014 Senomyx , ... company using proprietary taste science technologies to discover, ... food, beverage, and ingredient supply industries, today reported ... Company ended the second quarter with $33.6 million ... "During the past quarter Senomyx achieved another ...
(Date:7/31/2014)... global market for molecular diagnostics is expected to reach ... study by Grand View Research, Inc. Growing demand for ... advanced cancer diagnostic technologies are expected to be key ... Moreover, the growing global base of geriatric population and ... lifestyle habits such as smoking and excessive alcohol consumption ...
(Date:7/31/2014)... Miami, Fla. (PRWEB) July 31, 2014 The ... for distribution of its monthly news magazine, Kidney Cancer News. ... have a presence on a website maintained by an international ... noted an immediate increase in viewers.” , This month’s program ... of kidney tumors analyzed in a recent study. Kidney ...
(Date:7/30/2014)... 2014 W. R. Grace & ... the REVELERIS® Prep purification system , a ... and other researchers to perform both flash and ... , With the REVELERIS® Prep purification system, researchers ... modes with a simple touch of the screen, ...
Breaking Biology Technology:SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 2SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 3SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 4SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 5SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 6SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 7SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 8SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 9SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 10SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 11SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 12SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 13SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 14SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 15SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 16Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 2Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 3Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 4Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 5Grace Launches New REVELERIS® Prep Purification System 2
... to launch product immediately, NATICK, Mass., April ... today announced that it has received approval for,the ... in Canada. The Company reported that Health Canada,s ... and medical devices, has approved its use. The ...
... Product Pipeline with Increased Investment in ... Research & Development, DEERFIELD, Ill., ... financial results for,the first quarter of 2008, and raised its financial outlook ... of $429 million, a 6,percent increase compared to $403 million in the ...
... April 17 Syngenta today,announced that it will build ... Its focus will be on early-stage evaluation of GM ... soy, in the areas of yield,improvement, drought resistance, disease ... have a global scope and will complement,Syngenta,s biotech research ...
Cached Biology Technology:Boston Scientific Receives Canadian Approval for TAXUS(R) Liberte(R) Stent System 2Boston Scientific Receives Canadian Approval for TAXUS(R) Liberte(R) Stent System 3Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 2Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 3Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 4Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 5Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 6Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 7Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 8Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 9Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 10Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 11Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 12Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 13Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 14Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 15Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 16Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 17Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 18Baxter Reports Solid First Quarter Financial Results and Raises Full-Year Outlook 19Syngenta To Build Major Global Biotech Research Center in Beijing, China 2Syngenta To Build Major Global Biotech Research Center in Beijing, China 3
(Date:7/30/2014)... DURHAM, N.C. -- A study of dominance in female baboons ... maintain close ties with mom, and to have lots of ... not by size or strength, but by the rank of ... higher-ranked her daughter will be. For this reason, dominance rank ... Females born to high-ranking mothers are guaranteed a good spot ...
(Date:7/30/2014)... University associate professor Andrew S. Mount, performed cutting-edge research ... for novel anti-fouling paint for ships and boats and ... The team,s findings, published in Nature Communications ... that attaches to a wide variety of surfaces using ... an underwater heavy-duty adhesive. , "In previous research, we ...
(Date:7/30/2014)... July 30, 2014 ResearchMoz ... Government Biometric Systems Market 2014-2024   Business Strategies, Industry ... Biometric Systems Market 2014-2024" offers the reader detailed ... the next ten years, alongside potential market opportunities ... forecasts. To view the table of ...
Breaking Biology News(10 mins):Supportive moms and sisters boost female baboon's rank 2Supportive moms and sisters boost female baboon's rank 3Study: Marine pest provides advances in maritime anti-fouling and biomedicine 2Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 2Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 3Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 4Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 5Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 6Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 7Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 8Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 9Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 10
... in the Asian tsunami tragedy should be allowed to ... according to some of the world's leading scientists. , ... of Newcastle upon Tyne, and who set out their ... point to historical records of major coral reef devastation ...
... U.S. ports, a public enemy has infiltrated the nation's borders. ... Cornell University expert, the adult female alien is the only ... States. , The discovery of a single specimen of Sirex ... the nation because the invasive insect species has devastated up ...
... strains of leprosy-causing bacteria has revealed some surprises about ... across the continents by human migrations. The research, led ... in the 13 May issue of the journal Science, ... findings indicate that the world's existing leprosy infections are ...
Cached Biology News:Tsunami-damaged coral reefs should be left to recover naturally, say scientists 2Alien woodwasp, threat to US pine trees, found in N.Y. 2Leprosy genome tells story of human migrations, French researchers report in Science 2Leprosy genome tells story of human migrations, French researchers report in Science 3
Rat Antibody to Kinase Suppressor Of Ras Recognises the kinase suppressor of Ras (KSR). Immunogen: Recombinant human KSR protein Specificity: human kinase supressor of Ras...
... clone E978 GenBank ... Immunogen : NY-ESO-1 ... mouse ascites with 0.05% ... routinely evaluated by immunoblot ...
Form: Ready to use Applications: Immunohistology-frozen, Immunohistology-paraffin, Western Blot, ELISA...
EnzChek Ultra Xylanase Assay Kit *500 assays*...
Biology Products: