Navigation Links
Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived,,,Tissue

counter staining of the tissue is necessary, it is important to use a stain that will not be solubilized by the Situ/Mount product, such as BC50 Red stain.

Conclusions

RTISPCR is a recently developed approach for detecting rare s that often cannot be detected by traditional approaches, including in situ hybridization. RTISPCR can be used on archival tissues of almost any age, thereby giving new life to the samples maintained by pathology departments. In this study, we used RTISPCR to identify the expression of GRP receptors by mucosal epithelial cells in a human colonic polyp (figure 2). Our results suggest that expression of the GRP receptor may occur early in the development of colon cancer.

REFERENCES

  1. Ghatei, M.A., et al. (1982) J. Clin. Endocrinol. Metab. 54: 980985.

  2. Kaneto, A., Kaneto, T., Nakaya, S., et al.(1978) Metabolism 27: 549553.

  3. Falconieri Esrpamer, G., et al.(1988) Regul. Pept. 21: 111.

  4. Vigna, S.R., et al. (1987) Gastroenterology 93: 12871295.

  5. Ferris, H.A., Carroll, R.E., Lorimer, D.D., and Benya, R.V. (1997) Peptides, in press.

  6. Radulovic, S.S., Milovanovic, S.R., Cai, S.Z., et al. (1992) Proc. Soc. Exp. Biol. Med. 200: 394401.

  7. Radulovic, S., Miller, G., and Schally, A.V. (1991) Cancer Res. 51: 60066009.

  8. Frucht, H., Gazdar, A., and Jensen, R.T. (1991) Proc. Am. Assoc. Cancer Res. 32: 4752.

  9. Nuovo, G. (1994) Biochemica 11: 46.

  10. Heniford, B.W., ShumSiu, A., Leonberger, M., et al.(1993) Nucleic Acids Res. 21: 156166.


    11. '"/>


Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
2. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
3. Detecting miRNA & siRNA
4. Detecting Attomole Amounts of Small RNA
5. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
6. Efficient Primary Structure Elucidation of Disulfide-bridged Peptides
7. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
8. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
9. Complex Peptide Mixture Analysis on the Finnigan LTQ Linear Ion Trap Mass Spectrometer
10. Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80
11. LC-MALDI and MALDI-MS/MS for the Analysis of Complex Peptide Mixtures
Post Your Comments:
(Date:12/3/2009)NEW DELHI, India, Dec. 3 /PRNewswire-Asia/ -- Dr. Susan Jiang, M.D., Ph.D.,Chief Science Officer at Shenzhen Beike Biotechnology Co. Ltd.,( http://www.beikebiotech.com ), spoke at a conference on Hig
(Date:12/2/2009)NEW YORK, Dec. 2 Reportlinker.com announces that a new market research report is available in its catalogue: ,, Drug Discovery Technologies - A Global Update of Market Trends & Opportunities ,,
(Date:12/2/2009)LIBREVILLE, Gabon, December 2 , - "Bolstering Biodiversity in Africa: Everyone,s Problem and Heritage" , Coinciding with the International Year of Biodiversity, 2-3 June 2010 the,Gabonese Rep
(Date:12/2/2009)RESTON, Va., Dec. 2 /PRNewswire-Asia-FirstCall/ -- Emerald Dairy, Inc.,("Emerald Dairy" or the "Company") (OTC Bulletin Board: EMDY), a leading,producer and distributor of infant and children,s formul
Breaking Biology Technology:Beike Biotechnology CSO Speaks on Role of Stem Cells in Treating Diabetic Foot Disease at IPA Diabetic Foot Care Conference, India 2Beike Biotechnology CSO Speaks on Role of Stem Cells in Treating Diabetic Foot Disease at IPA Diabetic Foot Care Conference, India 3Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 2Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 3Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 4Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 5Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 6Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 7Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 8Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 9Libreville International Conference 2-3 June 2010 2Emerald Dairy To Be Featured in Spring Issue of US Food and Drink Executive 2
(Date:12/4/2009)UVALDE Dr. Daniel Leskovar, a Texas AgriLife Research plant physiologist at the Texas AgriLife Research and Extension Center in Uvalde, has been investigating ways to help vegetable plants make a les
(Date:12/4/2009)By measuring the total gene activity in organs relevant for coronary artery disease (CAD), scientists at the Swedish medical university Karolinska Institutet have identified a module of genes that is
(Date:12/4/2009)The electroencephalogram (EEG) is widely used by physicians and scientists to study brain function and to diagnose neurological disorders. However, it has remained largely unknown whether the electrod
Breaking Biology News(10 mins):Texas AgriLife researchers helping 2Texas AgriLife researchers helping 3Texas AgriLife researchers helping 4Scientists discover gene module underlying atherosclerosis development 2How to read brain activity? 2New species of ghostshark from California and Baja California 10028 1New species of ghostshark from California and Baja California 10028 2Explaining why pruning encourages plants to thrive 10026 1Explaining why pruning encourages plants to thrive 10026 2Selexis and Neogenix Oncology Announce Agreement on Antibody Project 57950 1Selexis and Neogenix Oncology Announce Agreement on Antibody Project 57950 2
...oduced by a patented microwave process which makes..., even cell attachment, increased cell growth and ...th uunder difficult conditions, including reduced ...ovide a more ecominic alternative to expensive and...
...es are designed for quick and easy protein purific...re two 0.25mm holes laser cut at the conical ends ... incubation of the gel matrix with buffer, during ...the buffer. Following a centrifugation step, the p...
...-art in permanent mounting media. It is designed f...al organic insoluble substrates such as diaminoben... organic solvent compatible chromogen substrates o...y and cytology applications using manual and autom...
...ndustry's first siRNA sequence design software in ...ls consistent with new experimental strategies and... With the introduction of our new, enhanced, and e...to offer the most significant advancement in RNAi ...
Biology Products: