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Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived,,,Tissue

counter staining of the tissue is necessary, it is important to use a stain that will not be solubilized by the Situ/Mount product, such as BC50 Red stain.

Conclusions

RTISPCR is a recently developed approach for detecting rare s that often cannot be detected by traditional approaches, including in situ hybridization. RTISPCR can be used on archival tissues of almost any age, thereby giving new life to the samples maintained by pathology departments. In this study, we used RTISPCR to identify the expression of GRP receptors by mucosal epithelial cells in a human colonic polyp (figure 2). Our results suggest that expression of the GRP receptor may occur early in the development of colon cancer.

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